The variation on nitrogen removal mechanisms and the succession of ammonia oxidizing archaea and ammonia oxidizing bacteria with temperature in biofilm reactors treating saline wastewater

2020 ◽  
Vol 314 ◽  
pp. 123760 ◽  
Author(s):  
Ziyuan Lin ◽  
Wei Huang ◽  
Jiong Zhou ◽  
Xuejie He ◽  
Jiale Wang ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Ammonia Oxidizing Bacteria ◽  
Saline Wastewater ◽  
Biofilm Reactors ◽  
Ammonia Oxidizing Archaea ◽  
Removal Mechanisms

Autotrophic nitrogen removal in sequencing batch biofilm reactors at different oxygen supply modes

2008 ◽  
Vol 58 (10) ◽  
pp. 1889-1894 ◽  
Author(s):  
C. Wantawin ◽  
J. Juateea ◽  
P. L. Noophan ◽  
J. Munakata-Marr
Keyword(s):  
Nitrogen Removal ◽  
Nitrogen Loss ◽  
Ammonia Concentration ◽  
Anammox Bacteria ◽  
Ammonia Oxidizing Bacteria ◽  
Intermittent Aeration ◽  
Biofilm Reactors ◽  
Air Supply ◽  
Nitrite Oxidizing Bacteria ◽  
Sequencing Batch Biofilm Reactors

Conventional nitrification-denitrification treatment is a common way to treat nitrogen in wastewater, but this process is costly for low COD/N wastewaters due to the addition of air and external carbon-source. However, ammonia may alternatively be converted to dinitrogen gas by autotrophic bacteria utilizing aerobically autotrophically produced nitrite as an electron acceptor under anoxic conditions. Lab-scale sequencing batch biofilm reactors (SBBRs) inoculated with normal nitrifying sludge were employed to study the potential of an oxygen-limited autotrophic nitrification-denitrification process initiated with typical nitrifying sludge for treating a synthetic ammonia wastewater devoid of organic carbon in one step. The ring-laced fibrous carrier (length 0.32 m, surface area 3.4 m2/m) was fixed vertically in a 3 L reactor. Two different air supply modes were applied:continuous aeration to control dissolved oxygen at 1.5 mg/L and intermittent aeration. High nitrogen removals of more than 50% were obtained in both SBBRs. At an ammonia loading of 0.882 gm N/m2-day [hydraulic retention time (HRT) of 24 hr], the SBBR continuously aerated to 1.5 mg DO/L had slightly higher nitrogen removal (64%) than the intermittently alternated SBBR (55%). The main form of residual nitrogen in the effluent was ammonia, at concentrations of 25 mg/L and 37 mg N/L in continuous and intermittent aeration SBBRs, respectively. Ammonia was completely consumed when ammonia loading was reduced to 0.441 gm N/m2-day [HRT extended to 48 hr]. The competitive use of nitrite by aerobic nitrite oxidizing bacteria (ANOB) with anaerobic ammonia-oxidizing bacteria (anammox bacteria) during the expanded aeration period under low remaining ammonia concentration resulted in higher nitrate production and lower nitrogen loss in the continuous aeration SBBR than in the intermittent aeration SBBR. The nitrogen removal efficiencies in SBBRs with continuous and alternating aerated were 80% and 86% respectively. Specific microorganisms in the biofilm were characterized using fluorescence in situ hybridization. Aerobic ammonia-oxidizing bacteria (AAOB) occurred side by side with putative anammox bacteria (cells hybridizing with probe AMX820) throughout the biofilm, though ANOB were rarely detected.

scholarly journals Ammonia-Oxidizing Archaea (AOA) Play with Ammonia-Oxidizing Bacteria (AOB) in Nitrogen Removal from Wastewater

Archaea ◽  
2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Zhixuan Yin ◽  
Xuejun Bi ◽  
Chenlu Xu
Keyword(s):  
Nitrogen Removal ◽  
Current Knowledge ◽  
Ammonia Oxidizing Bacteria ◽  
Low Oxygen ◽  
Factors Affecting ◽  
Future Studies ◽  
Ammonia Oxidizing Archaea ◽  
Removal Processes ◽  
Number Of Publications ◽  
Wastewater Treatment Systems

An increase in the number of publications in recent years indicates that besides ammonia-oxidizing bacteria (AOB), ammonia-oxidizing archaea (AOA) may play an important role in nitrogen removal from wastewater, gaining wide attention in the wastewater engineering field. This paper reviews the current knowledge on AOA and AOB involved in wastewater treatment systems and summarises the environmental factors affecting AOA and AOB. Current findings reveal that AOA have stronger environmental adaptability compared with AOB under extreme environmental conditions (such as low temperature and low oxygen level). However, there is still little information on the cooperation and competition relationship between AOA and AOB, and other microbes related to nitrogen removal, which needs further exploration. Furthermore, future studies are proposed to develop novel nitrogen removal processes dominated by AOA by parameter optimization.

scholarly journals A Review of Ammonia-Oxidizing Archaea and Anaerobic Ammonia-Oxidizing Bacteria in the Aquaculture Pond Environment in China

2021 ◽  
Vol 12 ◽  
Author(s):  
Shimin Lu ◽  
Xingguo Liu ◽  
Chong Liu ◽  
Guofeng Cheng ◽  
Runfeng Zhou ◽  
...  
Keyword(s):  
Water Treatment ◽  
Nitrogen Removal ◽  
Pond Water ◽  
Ammonia Oxidizing Bacteria ◽  
Pond Aquaculture ◽  
Environmentally Sustainable ◽  
Aquaculture Pond ◽  
Ammonia Oxidizing Archaea ◽  
Aquaculture Water ◽  
Sediment Layers

The excessive ammonia produced in pond aquaculture processes cannot be ignored. In this review, we present the distribution and diversity of ammonia-oxidizing archaea (AOA) and anaerobic ammonia-oxidizing bacteria (AnAOB) in the pond environment. Combined with environmental conditions, we analyze the advantages of AOA and AnAOB in aquaculture water treatment and discuss the current situation of pond water treatment engineering involving these microbes. AOA and AnAOB play an important role in the nitrogen removal process of aquaculture pond water, especially in seasonal low temperatures and anoxic sediment layers. Finally, we prospect the application of bioreactors to purify pond aquaculture water using AOA and AnAOB, in autotrophic nitrogen removal, which can reduce the production of greenhouse gases (such as nitrous oxide) and is conducive to the development of environmentally sustainable pond aquaculture.

scholarly journals Ammonia-oxidizing archaea are integral to nitrogen cycling in a highly fertile agricultural soil

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Laibin Huang ◽  
Seemanti Chakrabarti ◽  
Jennifer Cooper ◽  
Ana Perez ◽  
Sophia M. John ◽  
...  
Keyword(s):  
Nitrogen Cycle ◽  
Agricultural Soil ◽  
Agricultural Soils ◽  
Ammonia Oxidizing Bacteria ◽  
Central Process ◽  
Soil Nitrification ◽  
Plant Nitrogen ◽  
Ammonia Oxidizing Archaea ◽  
Activity Measurements ◽  
Nitrite Oxidizing Bacteria

AbstractNitrification is a central process in the global nitrogen cycle, carried out by a complex network of ammonia-oxidizing archaea (AOA), ammonia-oxidizing bacteria (AOB), complete ammonia-oxidizing (comammox) bacteria, and nitrite-oxidizing bacteria (NOB). Nitrification is responsible for significant nitrogen leaching and N2O emissions and thought to impede plant nitrogen use efficiency in agricultural systems. However, the actual contribution of each nitrifier group to net rates and N2O emissions remain poorly understood. We hypothesized that highly fertile agricultural soils with high organic matter mineralization rates could allow a detailed characterization of N cycling in these soils. Using a combination of molecular and activity measurements, we show that in a mixed AOA, AOB, and comammox community, AOA outnumbered low diversity assemblages of AOB and comammox 50- to 430-fold, and strongly dominated net nitrification activities with low N2O yields between 0.18 and 0.41 ng N2O–N per µg NOx–N in cropped, fallow, as well as native soil. Nitrification rates were not significantly different in plant-covered and fallow plots. Mass balance calculations indicated that plants relied heavily on nitrate, and not ammonium as primary nitrogen source in these soils. Together, these results imply AOA as integral part of the nitrogen cycle in a highly fertile agricultural soil.

scholarly journals Light as a Novel Inhibitor of Nitrite-Oxidizing Bacteria (NOB) for the Mainstream Partial Nitrification of Wastewater Treatment

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 346
Author(s):  
Keugtae Kim ◽  
Yong-Gyun Park
Keyword(s):  
Wastewater Treatment ◽  
Blue Light ◽  
Nitrogen Removal ◽  
Municipal Wastewater ◽  
Partial Nitrification ◽  
Biological Nutrient Removal ◽  
Nitrite Accumulation ◽  
Light Illumination ◽  
Ammonia Oxidizing Bacteria ◽  
Nitrite Oxidizing Bacteria

Conventional biological nutrient removal processes in municipal wastewater treatment plants are energy-consuming, with oxygen supply accounting for 45–75% of the energy expenditure. Many recent studies examined the implications of the anammox process in sidestream wastewater treatment to reduce energy consumption, however, the process did not successfully remove nitrogen in mainstream wastewater treatment with relatively low ammonia concentrations. In this study, blue light was applied as an inhibitor of nitrite-oxidizing bacteria (NOB) in a photo sequencing batch reactor (PSBR) containing raw wastewater. This simulated a biological nitrogen removal system for the investigation of its application potential in nitrite accumulation and nitrogen removal. It was found that blue light illumination effectively inhibited NOB rather than ammonia-oxidizing bacteria due to their different sensitivity to light, resulting in partial nitrification. It was also observed that the NOB inhibition rates were affected by other operational parameters like mixed liquor suspended solids (MLSS) concentration and sludge retention time (SRT). According to the obtained results, it was concluded that the process efficiency of partial nitrification and anammox (PN/A) could be significantly enhanced by blue light illumination with appropriate MLSS concentration and SRT conditions.

Screening and Influencing Factors of Halophilic Denitrifying Bacteria

2012 ◽  
Vol 550-553 ◽  
pp. 1455-1459
Author(s):  
Jing Tang ◽  
Hong Ming E ◽  
Jin Xiang Fu ◽  
Jin Nan Chen ◽  
Ming Fan
Keyword(s):  
Nitrogen Removal ◽  
Morphological Characteristics ◽  
Development Trend ◽  
Denitrifying Bacteria ◽  
High Salt ◽  
Research Progress ◽  
Saline Wastewater ◽  
Carbon And Nitrogen ◽  
Petroleum Chemical ◽  
Seafood Processing

With nitrite or nitrate nitrogen as electron acceptor in the high salt conditons, halophilic denitrifying bacteria can transfer nitrite or nitrate to nitrogen, thereby purifying the high-salt wastewater. Halophilic denitrifying bacteria play an important role in the carbon and nitrogen removal of saline wastewater, such as petroleum, chemical industry, seafood processing and seafood farming. This article dissussed halophilic denitrifying bacteria screening, the main types and the corresponding morphological characteristics, then we focused on the research progress of main factors of halophilic denitrifying bacteria’s growth and nitrogen removal. Finally put forward the current problems of the research and development trend of halophilic denitrifying bacteria.

scholarly journals Functional Characterization and Diversity of Ammonia-Oxidizing Microorganisms in Streams South of the Dabie Mountains, China

2018 ◽  
Author(s):  
Amjed Ginawi ◽  
Wang Lixiao ◽  
Huading Wang ◽  
Bingbing Yu ◽  
Yan Yunjun
Keyword(s):  
Quantitative Polymerase Chain Reaction ◽  
Functional Characterization ◽  
Physiological Adaptation ◽  
Ammonia Oxidizing Bacteria ◽  
Dabie Mountains ◽  
Natural Habitats ◽  
Environmental Pressures ◽  
Microbial Structures ◽  
Ammonia Oxidizing Archaea ◽  
Positive Correlations

Ammonia-oxidizing microorganism communities are abundant and functionally efficacious in nitrification. However, ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) groups complicate this process in subtropical streams. This study investigates the abundance of ammonia-oxidizing communities south of the Dabie Mountains, China, using quantitative polymerase chain reaction (qPCR). Clone libraries were utilized to analyze the abundance and microbial structures of AOA and AOB in sediments. Such analysis may provide strong evidence reflecting the links within the environment. The results show that AOB had a lower abundance of copies of the ammonia-oxidizing gene (amoA) than AOA. Interestingly, the AOA and AOB community compositions were correlated with ecological characteristics. The dissolved oxygen (DO) and oxidation-reduction potential (ORP) had significant positive correlations, whereas the phosphorus within the structure had a negative correlation with the abundance of both groups. Our study shows that it might adopt some species related to Nitrosotalea clusters that can resist comparably higher pH (toward pH 6.5). Together, these results imply that the physiological adaptation of microbial guilds to environmental pressures in ammonia-oxidizing archaea might allow them to have a more substantial function of ammonia-oxidizing communities in natural habitats.

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