Overexpression of SFA1 in engineered Saccharomyces cerevisiae to increase xylose utilization and ethanol production from different lignocellulose hydrolysates

2020 ◽  
Vol 313 ◽  
pp. 123724 ◽  
Author(s):  
Lang Zhu ◽  
Pengsong Li ◽  
Tongming Sun ◽  
Meilin Kong ◽  
Xiaowei Li ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Xylose Utilization ◽  
Engineered Saccharomyces Cerevisiae ◽  
Lignocellulose Hydrolysates

scholarly journals Enhanced xylose fermentation and ethanol production by engineered Saccharomyces cerevisiae strain

AMB Express ◽  
2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Leonardo de Figueiredo Vilela ◽  
Verônica Parente Gomes de Araujo ◽  
Raquel de Sousa Paredes ◽  
Elba Pinto da Silva Bon ◽  
Fernando Araripe Gonçalves Torres ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Xylose Fermentation ◽  
Engineered Saccharomyces Cerevisiae

scholarly journals Directed Evolution of Xylose Isomerase for Improved Xylose Catabolism and Fermentation in the Yeast Saccharomyces cerevisiae

2012 ◽  
Vol 78 (16) ◽  
pp. 5708-5716 ◽  
Author(s):  
Sun-Mi Lee ◽  
Taylor Jellison ◽  
Hal S. Alper
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Directed Evolution ◽  
Ethanol Yield ◽  
Xylose Isomerase ◽  
Mutant Enzyme ◽  
Xylose Utilization ◽  
Xylose Consumption ◽  
Content Type ◽  
Consumption Rates

ABSTRACTThe heterologous expression of a highly functional xylose isomerase pathway inSaccharomyces cerevisiaewould have significant advantages for ethanol yield, since the pathway bypasses cofactor requirements found in the traditionally used oxidoreductase pathways. However, nearly all reported xylose isomerase-based pathways inS. cerevisiaesuffer from poor ethanol productivity, low xylose consumption rates, and poor cell growth compared with an oxidoreductase pathway and, additionally, often require adaptive strain evolution. Here, we report on the directed evolution of thePiromycessp. xylose isomerase (encoded byxylA) for use in yeast. After three rounds of mutagenesis and growth-based screening, we isolated a variant containing six mutations (E15D, E114G, E129D, T142S, A177T, and V433I) that exhibited a 77% increase in enzymatic activity. When expressed in a minimally engineered yeast host containing agre3knockout andtal1andXKS1overexpression, the strain expressing this mutant enzyme improved its aerobic growth rate by 61-fold and both ethanol production and xylose consumption rates by nearly 8-fold. Moreover, the mutant enzyme enabled ethanol production by these yeasts under oxygen-limited fermentation conditions, unlike the wild-type enzyme. Under microaerobic conditions, the ethanol production rates of the strain expressing the mutant xylose isomerase were considerably higher than previously reported values for yeast harboring a xylose isomerase pathway and were also comparable to those of the strains harboring an oxidoreductase pathway. Consequently, this study shows the potential to evolve a xylose isomerase pathway for more efficient xylose utilization.

Regulation of Xylose Utilization in Recombinant Saccharomyces cerevisiae and Improvement of its Ethanol Production

2010 ◽  
Vol 150 ◽  
pp. 554-555
Author(s):  
Ji-Hye Han ◽  
Ju-Yong Park ◽  
Hyun Woo Kang ◽  
Gi-Wook Choi ◽  
Bong-Woo Chung ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Xylose Utilization ◽  
Recombinant Saccharomyces Cerevisiae

Platform of direct ethanol production from macroalgae by engineered Saccharomyces cerevisiae

2016 ◽  
Vol 33 ◽  
pp. S51 ◽  
Author(s):  
Keisuke Motone ◽  
Toshiyuki Takagi ◽  
Yusuke Sasaki ◽  
Kouichi Kuroda ◽  
Mitsuyoshi Ueda
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Engineered Saccharomyces Cerevisiae
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