Evaluation of the matrix effect of thermophilic anaerobic digestion on inactivation of infectious laryngotracheitis virus using real-time PCR and viral cell culture

2012 ◽  
Vol 110 ◽  
pp. 692-696 ◽  
Author(s):  
Tiejun Gao ◽  
Evelyn Bowlby ◽  
Yupin Tong ◽  
John T.Y. Wu ◽  
Lester Wong ◽  
...  
Keyword(s):  
Cell Culture ◽  
Anaerobic Digestion ◽  
Real Time ◽  
Matrix Effect ◽  
Real Time Pcr ◽  
Infectious Laryngotracheitis Virus ◽  
Thermophilic Anaerobic Digestion ◽  
The Matrix ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus

scholarly journals Detection of Infectious Laryngotracheitis Virus by Real-Time PCR in Naturally and Experimentally Infected Chickens

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e67598 ◽  
Author(s):  
Yan Zhao ◽  
Congcong Kong ◽  
Xianlan Cui ◽  
Hongyu Cui ◽  
Xingming Shi ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Infectious Laryngotracheitis Virus ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus

scholarly journals Glycoprotein J of infectious laryngotracheitis virus is required for efficient egress of infectious virions from cells

2011 ◽  
Vol 92 (11) ◽  
pp. 2586-2589 ◽  
Author(s):  
Alice Mundt ◽  
Egbert Mundt ◽  
Robert J. Hogan ◽  
Maricarmen García
Keyword(s):  
Cell Culture ◽  
Allantoic Fluid ◽  
The United States ◽  
Deletion Mutants ◽  
United States Department ◽  
Department Of Agriculture ◽  
Infectious Laryngotracheitis Virus ◽  
Infectious Laryngotracheitis ◽  
Dna Insertion ◽  
Laryngotracheitis Virus

Glycoprotein J (gJ) of infectious laryngotracheitis virus (ILTV) represents a major viral antigen and is dispensable for replication in cell culture and chickens. We generated gJ deletion mutants derived from the United States Department of Agriculture standard challenge strain (USDA-ch), a GFP-expressing mutant GΔgJ, a gJ deletion mutant void of any foreign DNA insertion (BΔgJ) and a gJ rescue mutant gJR with US5 restored. GΔgJ, BΔgJ and gJR were characterized in cell culture and embryonated eggs. Entry kinetic assays showed that the gJ deletion mutants did not differ in their entry kinetics from gJR. Replication kinetics strongly indicated that gJ plays an important role during egress of the virus. Differences in the abilities of the mutants to replicate in chorioallantoic membranes of chicken embryos and to release infectious virus into the allantoic fluid supported a function of gJ during the egress of ILTV from infected cells.

Sign in / Sign up

Export Citation Format

Share Document