Production of multiple xylanolytic and cellulolytic enzymes by thermophilic fungus Myceliophthora sp. IMI 387099

2007 ◽  
Vol 98 (3) ◽  
pp. 504-510 ◽  
Author(s):  
A.K. Badhan ◽  
B.S. Chadha ◽  
Jatinder Kaur ◽  
H.S. Saini ◽  
M.K. Bhat
Keyword(s):  
Thermophilic Fungus ◽  
Cellulolytic Enzymes

scholarly journals A Novel CreA-Mediated Regulation Mechanism of Cellulase Expression in the Thermophilic Fungus Humicola insolens

2019 ◽  
Vol 20 (15) ◽  
pp. 3693 ◽  
Author(s):  
Xinxin Xu ◽  
Chao Fan ◽  
Liya Song ◽  
Jinyang Li ◽  
Yuan Chen ◽  
...  
Keyword(s):  
Xylanase Activity ◽  
Hyphal Growth ◽  
Expression Regulation ◽  
Thermophilic Fungus ◽  
Cellulolytic Enzymes ◽  
Regulation Mechanism ◽  
Thermophilic Fungi ◽  
Disruption Mutant ◽  
Humicola Insolens ◽  
Cellulase Expression

The thermophilic fungus Humicola insolens produces cellulolytic enzymes that are of great scientific and commercial interest; however, few reports have focused on its cellulase expression regulation mechanism. In this study, we constructed a creA gene (carbon catabolite repressor gene) disruption mutant strain of H. insolens that exhibited a reduced radial growth rate and stouter hyphae compared to the wild-type (WT) strain. The creA disruption mutant also expressed elevated pNPCase (cellobiohydrolase activities), pNPGase (β-glucosidase activities), and xylanase levels in non-inducing fermentation with glucose. Unlike other fungi, the H. insolens creA disruption mutant displayed lower FPase (filter paper activity), CMCase (carboxymethyl cellulose activity), pNPCase, and pNPGase activity than observed in the WT strain when fermentation was induced using Avicel, whereas its xylanase activity was higher than that of the parental strain. These results indicate that CreA acts as a crucial regulator of hyphal growth and is part of a unique cellulase expression regulation mechanism in H. insolens. These findings provide a new perspective to improve the understanding of carbon catabolite repression regulation mechanisms in cellulase expression, and enrich the knowledge of metabolism diversity and molecular regulation of carbon metabolism in thermophilic fungi.

Amylase hyperproduction by deregulated mutants of the thermophilic fungus Thermomyces lanuginosus

2002 ◽  
Vol 29 (2) ◽  
pp. 70-74
Author(s):  
K Rubinder ◽  
BS Chadha ◽  
N Singh ◽  
HS Saini ◽  
S Singh
Keyword(s):  
Thermophilic Fungus ◽  
Thermomyces Lanuginosus

scholarly journals Generation of highly amenable cellulose-Iβ via selective delignification of rice straw using a reusable cyclic ether-assisted deep eutectic solvent system

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chiranjeevi Thulluri ◽  
Ravi Balasubramaniam ◽  
Harshad Ravindra Velankar
Keyword(s):  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Deep Eutectic Solvent ◽  
Solvent System ◽  
Cyclic Ether ◽  
Cellulolytic Enzymes ◽  
Ammonium Bromide ◽  
Selective Delignification ◽  
Cellulose Iβ ◽  
Hydrolysis Of

AbstractCellulolytic enzymes can readily access the cellulosic component of lignocellulosic biomass after the removal of lignin during biomass pretreatment. The enzymatic hydrolysis of cellulose is necessary for generating monomeric sugars, which are then fermented into ethanol. In our study, a combination of a deep eutectic (DE) mixture (of 2-aminoethanol and tetra-n-butyl ammonium bromide) and a cyclic ether (tetrahydrofuran) was used for selective delignification of rice straw (RS) under mild conditions (100 °C). Pretreatment with DE-THF solvent system caused ~ 46% delignification whereas cellulose (~ 91%) and hemicellulose (~ 67%) recoveries remained higher. The new solvent system could be reused upto 10 subsequent cycles with the same effectivity. Interestingly, the DE-THF pretreated cellulose showed remarkable enzymatic hydrolysability, despite an increase in its crystallinity to 72.3%. Contrary to conventional pretreatments, we report for the first time that the enzymatic hydrolysis of pretreated cellulose is enhanced by the removal of lignin during DE-THF pretreatment, notwithstanding an increase in its crystallinity. The current study paves way for the development of newer strategies for biomass depolymerization with DES based solvents.

scholarly journals Co-culture of Vel1-overexpressed Trichoderma asperellum and Bacillus amyloliquefaciens: An eco-friendly strategy to hydrolyze the lignocellulose biomass in soil to enrich the soil fertility, plant growth and disease resistance

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Valliappan Karuppiah ◽  
Lu Zhixiang ◽  
Hongyi Liu ◽  
Murugappan Vallikkannu ◽  
Jie Chen
Keyword(s):  
Disease Resistance ◽  
Plant Growth ◽  
Corn Stover ◽  
Bacillus Amyloliquefaciens ◽  
Genetically Engineered ◽  
Cellulolytic Enzymes ◽  
Regulation Of Transcription ◽  
Trichoderma Asperellum ◽  
Biomass Hydrolysis ◽  
Lignocellulose Biodegradation

Abstract Background Retention of agricultural bio-mass residues without proper treatment could affect the subsequent plant growth. In the present investigation, the co-cultivation of genetically engineered T. asperellum and B. amyloliquefaciens has been employed for multiple benefits including the enrichment of lignocellulose biodegradation, plant growth, defense potential and disease resistance. Results The Vel1 gene predominantly regulates the secondary metabolites, sexual and asexual development as well as cellulases and polysaccharide hydrolases productions. Overexpression mutant of the Trichoderma asperellum Vel1 locus (TA OE-Vel1) enhanced the activity of FPAase, CMCase, PNPCase, PNPGase, xylanase I, and xylanase II through the regulation of transcription regulating factors and the activation of cellulase and xylanase encoding genes. Further, these genes were induced upon co-cultivation with Bacillus amyloliquefaciens (BA). The co-culture of TA OE-Vel1 + BA produced the best composition of enzymes and the highest biomass hydrolysis yield of 89.56 ± 0.61%. The co-culture of TA OE-Vel1 + BA increased the corn stover degradation by the secretion of cellulolytic enzymes and maintained the C/N ratio of the corn stover amended soil. Moreover, the TA OE-Vel1 + BA increased the maize plant growth, expression of defense gene and disease resistance against Fusarium verticillioides and Cohilohorus herostrophus. Conclusion The co-cultivation of genetically engineered T. asperellum and B. amyloliquefaciens could be utilized as a profound and meaningful technique for the retention of agro residues and subsequent plant growth.

scholarly journals A promiscuous glycosyltransferase generates poly-β-1,4-glucan derivatives that facilitate mass spectrometry-based detection of cellulolytic enzymes

2021 ◽  
Author(s):  
Gregory S Bulmer ◽  
Ashley Philip Mattey ◽  
Fabio Parmeggiani ◽  
Ryan Williams ◽  
Helene Ledru ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cellulolytic Enzymes

Promiscuous activity of a glycosyltransferase was exploited to polymerise glucose from UDP-glucose via the generation of β-1,4-glycosidic linkages. The biocatalyst was incorporated into biocatalytic cascades and chemo-enzymatic strategies to synthesise...

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