The action of the bacterial toxin, microcin B17, on DNA gyrase

Biochimie ◽  
2007 ◽  
Vol 89 (4) ◽  
pp. 500-507 ◽  
Author(s):  
William M. Parks ◽  
Andrew R. Bottrill ◽  
Olivier A. Pierrat ◽  
Marcus C. Durrant ◽  
Anthony Maxwell
Keyword(s):  
Dna Gyrase ◽  
Bacterial Toxin ◽  
Microcin B17

scholarly journals In vitro characterization of DNA gyrase inhibition by microcin B17 analogs with altered bisheterocyclic sites

2001 ◽  
Vol 98 (14) ◽  
pp. 7712-7717 ◽  
Author(s):  
D. B. Zamble ◽  
D. A. Miller ◽  
J. G. Heddle ◽  
A. Maxwell ◽  
C. T. Walsh ◽  
...  
Keyword(s):  
Dna Gyrase ◽  
In Vitro Characterization ◽  
Microcin B17

scholarly journals Architecture of Microcin B17 Synthetase: An Octameric Protein Complex Converting a Ribosomally Synthesized Peptide into a DNA Gyrase Poison

2019 ◽  
Vol 73 (4) ◽  
pp. 749-762.e5 ◽  
Author(s):  
Dmitry Ghilarov ◽  
Clare E.M. Stevenson ◽  
Dmitrii Y. Travin ◽  
Julia Piskunova ◽  
Marina Serebryakova ◽  
...  
Keyword(s):  
Protein Complex ◽  
Dna Gyrase ◽  
Microcin B17

scholarly journals Fragments of the Bacterial Toxin Microcin B17 as Gyrase Poisons

PLoS ONE ◽  
2013 ◽  
Vol 8 (4) ◽  
pp. e61459 ◽  
Author(s):  
Frédéric Collin ◽  
Robert E. Thompson ◽  
Katrina A. Jolliffe ◽  
Richard J. Payne ◽  
Anthony Maxwell
Keyword(s):  
Bacterial Toxin ◽  
Microcin B17

scholarly journals Posttranslational modifications in microcin B17 define an additional class of DNA gyrase inhibitor.

1994 ◽  
Vol 91 (10) ◽  
pp. 4519-4523 ◽  
Author(s):  
P. Yorgey ◽  
J. Lee ◽  
J. Kordel ◽  
E. Vivas ◽  
P. Warner ◽  
...  
Keyword(s):  
Posttranslational Modifications ◽  
Dna Gyrase ◽  
Microcin B17

scholarly journals Protective Effect of Qnr on Agents Other than Quinolones That Target DNA Gyrase

2015 ◽  
Vol 59 (11) ◽  
pp. 6689-6695 ◽  
Author(s):  
George A. Jacoby ◽  
Marian A. Corcoran ◽  
David C. Hooper
Keyword(s):  
Protective Effect ◽  
Dna Gyrase ◽  
Molecular Probes ◽  
Protective Activity ◽  
Topoisomerase Iv ◽  
Synthetic Compounds ◽  
Target Dna ◽  
Simocyclinone D8 ◽  
Microcin B17 ◽  
Insight Into

ABSTRACTQnr is a plasmid-encoded and chromosomally determined protein that protects DNA gyrase and topoisomerase IV from inhibition by quinolones. Despite its prevalence worldwide and existence prior to the discovery of quinolones, its native function is not known. Other synthetic compounds and natural products also target bacterial topoisomerases. A number were studied as molecular probes to gain insight into how Qnr acts. Qnr blocked inhibition by synthetic compounds with somewhat quinolone-like structure that target the GyrA subunit, such as the 2-pyridone ABT-719, the quinazoline-2,4-dione PD 0305970, and the spiropyrimidinetrione pyrazinyl-alkynyl-tetrahydroquinoline (PAT), indicating that Qnr is not strictly quinolone specific, but Qnr did not protect against GyrA-targeting simocyclinone D8 despite evidence that both simocyclinone D8 and Qnr affect DNA binding to gyrase. Qnr did not affect the activity of tricyclic pyrimidoindole or pyrazolopyridones, synthetic inhibitors of the GyrB subunit, or nonsynthetic GyrB inhibitors, such as coumermycin A1, novobiocin, gyramide A, or microcin B17.Thus, in this set of compounds the protective activity of Qnr was confined to those that, like quinolones, trap gyrase on DNA in cleaved complexes.

scholarly journals Prevalence of Plasmid-Mediated Quinolone Resistance

2003 ◽  
Vol 47 (2) ◽  
pp. 559-562 ◽  
Author(s):  
George A. Jacoby ◽  
Nancy Chow ◽  
Ken B. Waites
Keyword(s):  
Medical Center ◽  
Dna Gyrase ◽  
The United States ◽  
Quinolone Resistance ◽  
Clinical Strain ◽  
University Of Alabama ◽  
Repeat Family ◽  
Gyrase A ◽  
The University ◽  
Microcin B17

ABSTRACT Quinolone resistance encoded by the qnr gene and mediated by plasmid pMG252 was discovered in a clinical strain of Klebsiella pneumoniae that was isolated in 1994 at the University of Alabama at Birmingham Medical Center. The gene codes for a protein that protects DNA gyrase from quinolone inhibition and that belongs to the pentapeptide repeat family of proteins. The prevalence of the gene has been investigated by using PCR with qnr-specific primers with a sample of more than 350 gram-negative strains that originated in 18 countries and 24 states in the United States and that included many strains with plasmid-mediated AmpC or extended spectrum β-lactamase enzymes. qnr was found in isolates from the University of Alabama at Birmingham only during 6 months in 1994, despite the persistence of the gene for FOX-5 β-lactamase, which is linked to qnr on pMG252. Isolates from other locations were negative for qnr. The prevalence of mcbG in the same sample was also examined. mcbG encodes another member of the pentapeptide repeat family and is involved in immunity to microcin B17, which, like quinolones, targets DNA gyrase. A single clinical isolate contained mcbG on a transmissible R plasmid. This plasmid and one carrying the complete microcin B17 operon slightly decreased sparfloxacin susceptibility but had a much less protective effect than pMG252. Plasmid-mediated quinolone resistance was thus rare in the sample examined.

scholarly journals The peptide antibiotic microcin B17 induces double-strand cleavage of DNA mediated by E. coli DNA gyrase.

1991 ◽  
Vol 10 (2) ◽  
pp. 467-476 ◽  
Author(s):  
J. L. Vizán ◽  
C. Hernández-Chico ◽  
I. del Castillo ◽  
F. Moreno
Keyword(s):  
Dna Gyrase ◽  
Peptide Antibiotic ◽  
E Coli ◽  
Microcin B17

scholarly journals The Action of the Bacterial Toxin Microcin B17

2003 ◽  
Vol 278 (37) ◽  
pp. 35016-35023 ◽  
Author(s):  
Olivier A. Pierrat ◽  
Anthony Maxwell
Keyword(s):  
Bacterial Toxin ◽  
Microcin B17
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