Methylglyoxal synthase regulates cell elongation via alterations of cellular methylglyoxal and spermidine content in Bacillus subtilis

2017 ◽  
Vol 91 ◽  
pp. 14-28 ◽  
Author(s):  
Sang-Min Shin ◽  
Sung-Hyun Song ◽  
Jin-Woo Lee ◽  
Min-Kyu Kwak ◽  
Sa-Ouk Kang
Keyword(s):  
Bacillus Subtilis ◽  
Cell Elongation ◽  
Methylglyoxal Synthase

scholarly journals Analysis of Outgrowth of Bacillus subtilis Spores Lacking Penicillin-Binding Protein 2a

1998 ◽  
Vol 180 (24) ◽  
pp. 6493-6502 ◽  
Author(s):  
Thomas Murray ◽  
David L. Popham ◽  
Christine B. Pearson ◽  
Arthur R. Hand ◽  
Peter Setlow
Keyword(s):  
Bacillus Subtilis ◽  
Cell Elongation ◽  
Binding Protein ◽  
Divalent Cations ◽  
Cross Linking ◽  
Penicillin Binding Protein ◽  
Wild Type ◽  
Spore Outgrowth ◽  
Partial Redundancy

ABSTRACT The loss of Bacillus subtilis penicillin-binding protein (PBP) 2a, encoded by pbpA, was previously shown to slow spore outgrowth and result in an increased diameter of the outgrowing spore. Further analyses to define the defect inpbpA spore outgrowth have shown that (i) outgrowingpbpA spores exhibited only a slight defect in the rate of peptidoglycan (PG) synthesis compared to wild-type spores, but PG turnover was significantly slowed during outgrowth of pbpAspores; (ii) there was no difference in the location of PG synthesis in outgrowing wild-type and pbpA spores once cell elongation had been initiated; (iii) outgrowth and elongation of pbpAspores were dramatically affected by the levels of monovalent or divalent cations in the medium; (iv) there was a partial redundancy of function between PBP2a and PBP1 or -4 during spore outgrowth; and (v) there was no difference in the structure of PG from outgrowing wild-type spores or spores lacking PBP2a or PBP2a and -4; but also (vi) PG from outgrowing spores lacking PBP1 and -2a had transiently decreased cross-linking compared to PG from outgrowing wild-type spores, possibly due to the loss of transpeptidase activity.

scholarly journals MreB-Dependent Inhibition of Cell Elongation during the Escape from Competence in Bacillus subtilis

PLoS Genetics ◽  
2015 ◽  
Vol 11 (6) ◽  
pp. e1005299 ◽  
Author(s):  
Nicolas Mirouze ◽  
Cécile Ferret ◽  
Zhizhong Yao ◽  
Arnaud Chastanet ◽  
Rut Carballido-López
Keyword(s):  
Bacillus Subtilis ◽  
Cell Elongation ◽  
Dependent Inhibition

scholarly journals Control of the cell elongation–division cycle by shuttling of PBP1 protein in Bacillus subtilis

2008 ◽  
Vol 68 (4) ◽  
pp. 1029-1046 ◽  
Author(s):  
Dennis Claessen ◽  
Robyn Emmins ◽  
Leendert W. Hamoen ◽  
Richard A. Daniel ◽  
Jeff Errington ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Cell Elongation

CcpN: a moonlighting protein regulating catabolite repression of gluconeogenic genes in Bacillus subtilis also affects cell length and interacts with DivIVA

2020 ◽  
Vol 66 (12) ◽  
pp. 723-732
Author(s):  
Kusum Sharma ◽  
Taranum Sultana ◽  
Tanya E.S. Dahms ◽  
Jo-Anne R. Dillon
Keyword(s):  
Bacillus Subtilis ◽  
Cell Elongation ◽  
Regulatory Rna ◽  
Glutathione S Transferase ◽  
Biosynthesis Gene Cluster ◽  
Gram Positive Bacteria ◽  
Moonlighting Protein ◽  
Insertional Inactivation ◽  
Biosynthesis Gene ◽  
Two Hybrid

CcpN is a transcriptional repressor in Bacillus subtilis that binds to the promoter region of gapB and pckA, downregulating their expression in the presence of glucose. CcpN also represses sr1, which encodes a small noncoding regulatory RNA that suppresses the arginine biosynthesis gene cluster. CcpN has homologues in other Gram-positive bacteria, including Enterococcus faecalis. We report the interaction of CcpN with DivIVA of B. subtilis as determined using bacterial two-hybrid and glutathione S-transferase pull-down assays. Insertional inactivation of CcpN leads to cell elongation and formation of straight chains of cells. These findings suggest that CcpN is a moonlighting protein involved in both gluconeogenesis and cell elongation.

scholarly journals Metabolic Control of Cell Elongation and Cell Division in Bacillus subtilis

2021 ◽  
Vol 12 ◽  
Author(s):  
Anne Galinier ◽  
Elodie Foulquier ◽  
Frédérique Pompeo
Keyword(s):  
Cell Cycle ◽  
Bacillus Subtilis ◽  
Cell Division ◽  
Cell Elongation ◽  
Bacterial Cell ◽  
Doubling Time ◽  
Control Cell ◽  
Cellular Processes ◽  
Nutritional Conditions ◽  
Bacterial Cell Cycle

To survive and adapt to changing nutritional conditions, bacteria must rapidly modulate cell cycle processes, such as doubling time or cell size. Recent data have revealed that cellular metabolism is a central regulator of bacterial cell cycle. Indeed, proteins that can sense precursors or metabolites or enzymes, in addition to their enzymatic activities involved in metabolism, were shown to directly control cell cycle processes in response to changes in nutrient levels. Here we focus on cell elongation and cell division in the Gram-positive rod-shaped bacterium Bacillus subtilis and we report evidences linking these two cellular processes to environmental nutritional availability and thus metabolic cellular status.

Use of Antibody to aid Visualization of Protein at the Termini of Bacteriophage Ø29 DNA

1980 ◽  
Vol 38 ◽  
pp. 540-541
Author(s):  
Dwight Anderson ◽  
Charlene Peterson ◽  
Gursaran Notani ◽  
Bernard Reilly
Keyword(s):  
Electron Microscopy ◽  
Bacillus Subtilis ◽  
Dna Synthesis ◽  
Restriction Endonuclease ◽  
Protein Complex ◽  
Protein Product ◽  
Viral Dna ◽  
Small Proteins ◽  
Antibody Labeling ◽  
Subtilis Bacteriophage

The protein product of cistron 3 of Bacillus subtilis bacteriophage Ø29 is essential for viral DNA synthesis and is covalently bound to the 5’-termini of the Ø29 DNA. When the DNA-protein complex is cleaved with a restriction endonuclease, the protein is bound to the two terminal fragments. The 28,000 dalton protein can be visualized by electron microscopy as a small dot and often is seen only when two ends are in apposition as in multimers or in glutaraldehyde-fixed aggregates. We sought to improve the visibility of these small proteins by use of antibody labeling.

Antibacterial activity of Celastrol against Bacillus subtilis

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
N Padilla-Montaño ◽  
IL Bazzocchi ◽  
L Moujir
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis

Mikrobizide und viruzide Aufbereitung von Dialysegeräten

2018 ◽  
Vol 22 (02) ◽  
pp. 82-89
Author(s):  
Friedrich von Rheinbaben ◽  
Oliver Riebe ◽  
Johanna Köhnlein ◽  
Sebastian Werner
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Phase 3 ◽  
Aspergillus Brasiliensis

ZusammenfassungZentrales Bauteil des Genius® 90 Therapie Systems ist der sogenannte Genius-Tank, dem die frische Dialyseflüssigkeit entnommen und in den die verbrauchte Lösung nach der Dialyse zurückgeführt wird. Daher kommt der sicheren Aufbereitung des Systems eine besondere Bedeutung zu. Hierfür wird ein Aufbereitungsverfahren unter Verwendung von UV-Licht in Kombination mit einem chemischen Desinfektionsmittel angewendet. Ziel der hier beschriebenen Untersuchung war es, die Wirkungsbreite und Wirkungstiefe dieses Aufbereitungsverfahrens unter praxisnahen Phase-3-Bedingungen zu ermitteln. Dazu wurde das Gerät mit Mikroorganismen und Viren künstlich kontaminiert und die Wirkung der einzelnen Verfahrensschritte ermittelt. Im Gegensatz zu der üblichen Vorgehensweise praxisnaher Untersuchungen machen Aufbereitungsverfahren medizinischer Geräte unter Phase-3-Kriterien meist eine neuartige Arbeitsweise erforderlich – im Falle der hier vorgestellten Untersuchung sogar die Konstruktion eines speziellen Geräts zur Platzierung von Keimträgen im Genius-Tank. Im Ergebnis konnte gezeigt werden, dass bereits UV-Licht allein sowie in Kombination mit einem chemischen Desinfektionsmittel unter praxisnahen Bedingungen eine sichere Wirksamkeit gegen Bakterien (Pseudomonas aeruginosa) und bakterielle Sporen (Bacillus subtilis), Schimmelpilze (Aspergillus brasiliensis) und Viren (Murines Parvovirus) besitzt.

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