Functional analysis of BAS2108-2109 two component system: Evidence for protease regulation in Bacillus anthracis

2017 ◽  
Vol 89 ◽  
pp. 71-84 ◽  
Author(s):  
Vatika Gupta ◽  
Neha Chaudhary ◽  
Somya Aggarwal ◽  
Nidhi Adlakha ◽  
Pooja Gulati ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Bacillus Anthracis ◽  
Two Component System ◽  
Component System ◽  
Two Component

scholarly journals Two-Component System Cross-Regulation Integrates Bacillus anthracis Response to Heme and Cell Envelope Stress

2014 ◽  
Vol 10 (3) ◽  
pp. e1004044 ◽  
Author(s):  
Laura A. Mike ◽  
Jacob E. Choby ◽  
Paul R. Brinkman ◽  
Lorenzo Q. Olive ◽  
Brendan F. Dutter ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Cell Envelope ◽  
Two Component System ◽  
Component System ◽  
Envelope Stress ◽  
Two Component

Characterization of a two component system, Bas1213‐1214, important for oxidative stress in Bacillus anthracis

2018 ◽  
Vol 119 (7) ◽  
pp. 5761-5774 ◽  
Author(s):  
Vatika Gupta ◽  
Kanika Jain ◽  
Rajni Garg ◽  
Anshu Malik ◽  
Pooja Gulati ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bacillus Anthracis ◽  
Two Component System ◽  
Component System ◽  
Two Component

DnaJ and ClpX are required for HitRS and HssRS two-component system signaling in Bacillus anthracis

2021 ◽  
Author(s):  
Clare L. Laut ◽  
Catherine S. Leasure ◽  
Hualiang Pi ◽  
Sophia M. Carlin ◽  
Michelle L. Chu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Signal Transduction ◽  
Bacillus Anthracis ◽  
Cell Envelope ◽  
Genetic Selection ◽  
Selection Strategy ◽  
Two Component System ◽  
Component System ◽  
Two Component ◽  
Substantial Risk

Bacillus anthracis is the causative agent of anthrax. This Gram-positive bacterium poses a substantial risk to human health due to high mortality rates and the potential for malicious use as a bioterror weapon. To survive within the vertebrate host, B. anthracis relies on two-component system (TCS) signaling to sense host-induced stresses and respond to alterations in the environment through changes in target gene expression. HitRS and HssRS are cross-regulating TCSs in B. anthracis that respond to cell envelope disruptions and high heme levels, respectively. In this study, an unbiased and targeted genetic selection was designed to identify gene products that are involved in HitRS and HssRS signaling. This selection led to the identification of inactivating mutations within dnaJ and clpX that disrupt HitRS- and HssRS-dependent gene expression. DnaJ and ClpX are the substrate-binding subunits of the DnaJK protein chaperone and ClpXP protease, respectively. DnaJ regulates the levels of HitR and HitS to facilitate signal transduction, while ClpX specifically regulates HitS levels. Together these results reveal that the protein homeostasis regulators, DnaJ and ClpX, function to maintain B. anthracis signal transduction activities through TCS regulation. One sentence summary: Use of a genetic selection strategy to identify modulators of two-component system signaling in Bacillus anthracis .

scholarly journals Bacillus anthracis Responds to Targocil-Induced Envelope Damage through EdsRS Activation of Cardiolipin Synthesis

mBio ◽  
2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Clare L. Laut ◽  
William J. Perry ◽  
Alexander L. Metzger ◽  
Andy Weiss ◽  
Devin L. Stauff ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Transcriptional Activation ◽  
Structural Damage ◽  
Cell Damage ◽  
Cell Envelope ◽  
Two Component System ◽  
Component System ◽  
Content Type ◽  
Two Component ◽  
Cardiolipin Synthase

ABSTRACT Bacillus anthracis is a spore-forming bacterium that causes devastating infections and has been used as a bioterror agent. This pathogen can survive hostile environments through the signaling activity of two-component systems, which couple environmental sensing with transcriptional activation to initiate a coordinated response to stress. In this work, we describe the identification of a two-component system, EdsRS, which mediates the B. anthracis response to the antimicrobial compound targocil. Targocil is a cell envelope-targeting compound that is toxic to B. anthracis at high concentrations. Exposure to targocil causes damage to the cellular barrier and activates EdsRS to induce expression of a previously uncharacterized cardiolipin synthase, which we have named ClsT. Both EdsRS and ClsT are required for protection against targocil-dependent damage. Induction of clsT by EdsRS during targocil treatment results in an increase in cardiolipin levels, which protects B. anthracis from envelope damage. Together, these results reveal that a two-component system signaling response to an envelope-targeting antimicrobial induces production of a phospholipid associated with stabilization of the membrane. Cardiolipin is then used to repair envelope damage and promote B. anthracis viability. IMPORTANCE Compromising the integrity of the bacterial cell barrier is a common action of antimicrobials. Targocil is an antimicrobial that is active against the bacterial envelope. We hypothesized that Bacillus anthracis, a potential weapon of bioterror, senses and responds to targocil to alleviate targocil-dependent cell damage. Here, we show that targocil treatment increases the permeability of the cellular envelope and is particularly toxic to B. anthracis spores during outgrowth. In vegetative cells, two-component system signaling through EdsRS is activated by targocil. This results in an increase in the production of cardiolipin via a cardiolipin synthase, ClsT, which restores the loss of barrier function, thereby reducing the effectiveness of targocil. By elucidating the B. anthracis response to targocil, we have uncovered an intrinsic mechanism that this pathogen employs to resist toxicity and have revealed therapeutic targets that are important for bacterial defense against structural damage.

scholarly journals Identification, Functional Characterization and Regulon Prediction of a Novel Two Component System Comprising BAS0540-BAS0541 of Bacillus anthracis

PLoS ONE ◽  
2016 ◽  
Vol 11 (7) ◽  
pp. e0158895 ◽  
Author(s):  
Monisha Gopalani ◽  
Alisha Dhiman ◽  
Amit Rahi ◽  
Divya Kandari ◽  
Rakesh Bhatnagar
Keyword(s):  
Bacillus Anthracis ◽  
Functional Characterization ◽  
Two Component System ◽  
Component System ◽  
Two Component
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