Burst of succinate dehydrogenase and α-ketoglutarate dehydrogenase activity in concert with the expression of genes coding for respiratory chain proteins underlies short-term beneficial physiological stress in mitochondria

2013 ◽  
Vol 45 (1) ◽  
pp. 190-200 ◽  
Author(s):  
Marina V. Zakharchenko ◽  
A.V. Zakharchenko ◽  
N.V. Khunderyakova ◽  
M.N. Tutukina ◽  
M.A. Simonova ◽  
...  
Keyword(s):  
Succinate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Respiratory Chain ◽  
Physiological Stress ◽  
Short Term ◽  
Expression Of Genes ◽  
Ketoglutarate Dehydrogenase

Succinate Dehydrogenase Deficiency

2000 ◽  
Vol 124 (12) ◽  
pp. 1755-1758
Author(s):  
Georgirene D. Vladutiu ◽  
Reid R. Heffner
Keyword(s):  
Succinate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Respiratory Chain ◽  
Dehydrogenase Deficiency ◽  
Respiratory Chain Enzyme ◽  
Succinate Dehydrogenase Activity ◽  
Enzyme Defects ◽  
Chain Defects ◽  
Muscle Biopsies ◽  
Succinate Dehydrogenase Deficiency

Abstract Background.—Partial succinate dehydrogenase deficiency (15% to 50% of normal reference enzyme activity) in skeletal muscle causes mitochondrial myopathy with various symptoms, for example, brain involvement, cardiomyopathy, and/or exercise intolerance. The deficiency may be isolated or may coexist with other respiratory-chain enzyme defects. The histopathologic assessment of succinate dehydrogenase activity in muscle biopsies of patients with suspected mitochondrial myopathies has focused on the finding of increased staining, usually in ragged-red fibers, rather than on reduced staining. Objectives.—To determine the prevalence of muscle succinate dehydrogenase deficiency among patients with respiratory-chain defects and to determine whether the reduced activity is present histochemically and is comparable to the quantitative reduction found in muscle homogenates. Patients and Methods.—One hundred eight muscle biopsies were evaluated from patients with suspected mitochondrial myopathies by qualitative histochemical analysis and quantitative biochemical analyses of respiratory-chain enzymes using standard methodologies. Results.—Fifty-two patients had defects in respiratory-chain complexes; of these patients, 12 (23%) had partial deficiencies in succinate dehydrogenase activity either alone or together with reductions in other enzymes. The reduced activity was detectable histochemically in muscle biopsies with residual enzyme activity of up to 34% of the normal reference activity, while 2 biopsies with higher residual activity (49% and 68% of normal) could not be distinguished from normal biopsies. Conclusions—Of the patients with respiratory-chain enzyme defects, 23% had partial deficiencies of succinate dehydrogenase activity in muscle biopsies. This reduction could be detected histochemically in biopsies in most cases. The marked prevalence of succinate dehydrogenase deficiency among patients with respiratory-chain defects and its detection initially by histochemical analysis are important findings.

ACUTE ENZYME HISTOCHEMICAL CHANGES IN THE ZONA GLOMERULOSA OF THE RAT ADRENAL CORTEX

1968 ◽  
Vol 59 (3) ◽  
pp. 508-518
Author(s):  
J. D. Elema ◽  
M. J. Hardonk ◽  
Joh, Koudstaal ◽  
A. Arends
Keyword(s):  
Peritoneal Dialysis ◽  
Succinate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Adrenal Cortex ◽  
Isocitrate Dehydrogenase ◽  
Hydroxysteroid Dehydrogenase ◽  
Zona Glomerulosa ◽  
Acute Changes ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Histochemical Changes

ABSTRACT Acute changes in glucose-6-phosphate dehydrogenase and isocitrate dehydrogenase activity in the zona glomerulosa of the rat adrenal cortex were induced by peritoneal dialysis with 5 % glucose. Although less clear, the activity of 3β-ol-hydroxysteroid dehydrogenase also seemed to increase as well. No changes were seen in the activity of succinate dehydrogenase. Dialysis with 0.9 % NaCl had no effect on any of the enzymes investigated. The possible significance of these observations is discussed.

scholarly journals The probable site of action of thenolytrifluoracetone on the respiratory chain

1977 ◽  
Vol 164 (3) ◽  
pp. 617-620 ◽  
Author(s):  
W J Ingledew ◽  
T Ohnishi
Keyword(s):  
Succinate Dehydrogenase ◽  
Respiratory Chain ◽  
Close Association ◽  
Succinate Oxidation ◽  
Paramagnetic Resonance ◽  
Spin Interactions ◽  
Site Of Action ◽  
Transfer Inhibitor ◽  
Probable Site ◽  
Electron Paramagnetic

1. It is shown that the electron-transfer inhibitor thenoyltrifluoroacetone abolishes a respiratory-chain electron-paramagnetic-resonance absorbance due to spin-spin interactions of ubisemiquinones at concentrations similar to those required for inhibition of succinate oxidation. 2. A specific site of interaction of thenoyltrifluoroacetone with the respiratory chain is proposed to be on the ubisemiquinone with which succinate dehydrogenase reacts. 3. Our results further demonstrate the close association of the HiPIP (high-potential iron-sulphur) centre of succinate dehydrogenase with ubisemiquinone.

Short-term High-intensity Interval Training and The Physiological Stress Response

2011 ◽  
Vol 43 (Suppl 1) ◽  
pp. 905-906
Author(s):  
Michael J. Ormsbee ◽  
Amber W. Kinsey ◽  
Minwook Chong ◽  
Heather S. Friedman ◽  
Tonya Dodge ◽  
...  
Keyword(s):  
Stress Response ◽  
High Intensity ◽  
Physiological Stress ◽  
Interval Training ◽  
High Intensity Interval Training ◽  
Short Term ◽  
Physiological Stress Response ◽  
High Intensity Interval
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