Adenomatous polyposis coli protein regulates the cellular response to DNA replication stress

2011 ◽  
Vol 43 (9) ◽  
pp. 1354-1364 ◽  
Author(s):  
Mariana G. Brocardo ◽  
James A. Borowiec ◽  
Beric R. Henderson
Keyword(s):  
Dna Replication ◽  
Adenomatous Polyposis Coli ◽  
Cellular Response ◽  
Replication Stress ◽  
Adenomatous Polyposis ◽  
Polyposis Coli ◽  
Adenomatous Polyposis Coli Protein ◽  
Dna Replication Stress

scholarly journals The Adenomatous Polyposis Coli Protein Is Required for the Formation of Robust Spindles Formed in CSF Xenopus Extracts

2004 ◽  
Vol 15 (6) ◽  
pp. 2978-2991 ◽  
Author(s):  
Dina Dikovskaya ◽  
Ian P. Newton ◽  
Inke S. Näthke
Keyword(s):  
Adenomatous Polyposis Coli ◽  
Chromosomal Instability ◽  
Adenomatous Polyposis ◽  
Mitotic Spindles ◽  
Polyposis Coli ◽  
Adenomatous Polyposis Coli Protein ◽  
Microtubule Binding ◽  
Microtubule Polymerization ◽  
Large N ◽  
Apc Protein

Mutations in the adenomatous polyposis coli (APC) protein occur early in colon cancer and correlate with chromosomal instability. Here, we show that depletion of APC from cystostatic factor (CSF) Xenopus extracts leads to a decrease in microtubule density and changes in tubulin distribution in spindles and asters formed in such extracts. Addition of full-length APC protein or a large, N-terminally truncated APC fragment to APC-depleted extracts restored normal spindle morphology and the intact microtubule-binding site of APC was necessary for this rescue. These data indicate that the APC protein plays a role in the formation of spindles that is dependent on its effect on microtubules. Spindles formed in cycled extracts were not sensitive to APC depletion. In CSF extracts, spindles predominantly formed from aster-like intermediates, whereas in cycled extracts chromatin was the major site of initial microtubule polymerization. These data suggest that APC is important for centrosomally driven spindle formation, which was confirmed by our finding that APC depletion reduced the size of asters nucleated from isolated centrosomes. We propose that lack of microtubule binding in cancer-associated mutations of APC may contribute to defects in the assembly of mitotic spindles and lead to missegregation of chromosomes.

scholarly journals The Adenomatous Polyposis Coli Protein Contributes to Normal Compaction of Mitotic Chromatin

PLoS ONE ◽  
2012 ◽  
Vol 7 (6) ◽  
pp. e38102 ◽  
Author(s):  
Dina Dikovskaya ◽  
Guennadi Khoudoli ◽  
Ian P. Newton ◽  
Gaganmeet S. Chadha ◽  
Daniel Klotz ◽  
...  
Keyword(s):  
Adenomatous Polyposis Coli ◽  
Adenomatous Polyposis ◽  
Polyposis Coli ◽  
Adenomatous Polyposis Coli Protein ◽  
Mitotic Chromatin

Abstract LB-164: DNA ligase IV modulates the cellular response to DNA replication stress

2016 ◽  
Author(s):  
Grace Hooks ◽  
Yasmin Anchondo ◽  
Neelam Sharma ◽  
Jac Nickoloff ◽  
Amanda Ashley
Keyword(s):  
Dna Replication ◽  
Cellular Response ◽  
Replication Stress ◽  
Dna Ligase ◽  
Dna Ligase Iv ◽  
Ligase Iv ◽  
Dna Replication Stress

scholarly journals Regulated Binding of Adenomatous Polyposis Coli Protein to Actin

2007 ◽  
Vol 282 (17) ◽  
pp. 12661-12668 ◽  
Author(s):  
James B. Moseley ◽  
Francesca Bartolini ◽  
Kyoko Okada ◽  
Ying Wen ◽  
Gregg G. Gundersen ◽  
...  
Keyword(s):  
Adenomatous Polyposis Coli ◽  
Adenomatous Polyposis ◽  
Polyposis Coli ◽  
Adenomatous Polyposis Coli Protein
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