scholarly journals Keratinocyte serum-free medium maintains long-term liver gene expression and function in cultured rat hepatocytes by preventing the loss of liver-enriched transcription factors

2007 ◽  
Vol 39 (3) ◽  
pp. 541-554 ◽  
Author(s):  
Wan-Chun Li ◽  
Kate L. Ralphs ◽  
Jonathan M.W. Slack ◽  
David Tosh
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Rat Hepatocytes ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Cultured Rat Hepatocytes ◽  
Liver Gene ◽  
And Function

Long-term culture of rat hepatocytes on porous membranes in hormonally defined serum-free medium

1993 ◽  
Vol 7 (4) ◽  
pp. 453-459 ◽  
Author(s):  
C. Guery ◽  
J.P. Stepniewski ◽  
B. Vannier ◽  
R. Fournex ◽  
G. Lorenzon
Keyword(s):  
Rat Hepatocytes ◽  
Porous Membranes ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Long Term Culture

scholarly journals Induction of mRNA for phosphoenolpyruvate carboxykinase (GTP) by dexamethasone in cultured rat hepatocytes requires on-going protein synthesis

1987 ◽  
Vol 246 (1) ◽  
pp. 237-240 ◽  
Author(s):  
V L Nebes ◽  
S M Morris
Keyword(s):  
Phosphoenolpyruvate Carboxykinase ◽  
Rat Hepatocytes ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Maximum Induction ◽  
Cultured Rat Hepatocytes ◽  
Time Required ◽  
Necessary And Sufficient ◽  
Stimulation Of

Dexamethasone is necessary and sufficient to induce mRNA for phosphoenolpyruvate carboxykinase (GTP) (PEPCK) by 19-fold in rat hepatocytes cultured in serum-free medium. However, the time required for maximum induction is 16 h. The slow induction suggested that glucocorticoids regulate the expression of an intermediate gene product(s) which is required for glucocorticoid stimulation of PEPCK-gene expression. Consistent with this notion was the finding that cycloheximide completely blocked the response to dexamethasone. In contrast, cycloheximide did not block the response to a cyclic AMP analogue.

Receptors for epidermal growth factor (urogastrone) and insulin in primary cultures of rat hepatocytes maintained in serum-free medium

1986 ◽  
Vol 64 (8) ◽  
pp. 803-810 ◽  
Author(s):  
M. O'Connor-McCourt ◽  
M. Soley ◽  
L. J. Hayden ◽  
M. D. Hollenberg
Keyword(s):  
Growth Factor ◽  
Molecular Mass ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Serum Free Medium ◽  
Free Medium ◽  
Cross Link ◽  
Serum Free ◽  
Epidermal Growth

We have analyzed the receptors for epidermal growth factor (urogastrone) (EGF-URO) and insulin in primary cultures of adult rat hepatocytes maintained for up to 3 weeks on human placental cell matrix in serum-free defined medium. Cross-link labeling experiments revealed that the insulin receptor, partially damaged by the collagenase isolation procedure, was rapidly regenerated to yield an intact receptor. In contrast, cross-link labeling of the EGF-URO receptor revealed that, upon prolonged culture, there was a progressive disappearance of the high molecular mass (175 kilodaltons (kDa)) receptor form, and an appearance of low molecular mass receptor species (130 and 105 kDa). After 3 weeks of culture, the low molecular mass receptor forms accounted for all of the labeled EGF-URO receptor present in the cells. Measurements of EGF-URO binding indicated that the number of EGF-URO binding sites per cell (2.0 × 105 ± 0.3 × 105) did not change during the 3 weeks of culture. However, there was a decrease in EGF-URO binding affinity, reflected by an increase in the KD from 0.6 to 3.0 nM. At zero time and after 3 weeks in culture, Scatchard plots of the binding data were linear; at intermediate time points, the plots were curvilinear. Despite the changes in the EGF-URO receptor that occurred, cells were still responsive to EGF-URO in terms of the inhibition of acetate incorporation into lipid. The ED50 for EGF-URO (about 0.2 nM) was the same for short-term cultures (48 h) as for cells maintained in culture for 3 weeks. We conclude that the long-term culture of hepatocytes in serum-free medium yields an altered low molecular form of the EGF-URO receptor that is, nonetheless, functional. The study points to differential changes in receptors for peptide hormones that may occur in long-term hepatocyte cultures and illustrates the feasibility of using such cultures for metabolic studies of the actions of EGF-URO.

Gene expression changes during Giardia–host cell interactions in serum-free medium

2014 ◽  
Vol 197 (1-2) ◽  
pp. 21-23 ◽  
Author(s):  
Marcela Ferella ◽  
Barbara J. Davids ◽  
Michael J. Cipriano ◽  
Shanda R. Birkeland ◽  
Daniel Palm ◽  
...  
Keyword(s):  
Gene Expression ◽  
Host Cell ◽  
Cell Interactions ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Host Cell Interactions
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