scholarly journals Cellobionic acid inhibition of cellobiohydrolase I and cellobiose dehydrogenase

2016 ◽  
Vol 109 ◽  
pp. 236-242 ◽  
Author(s):  
Amanda Hildebrand ◽  
J. Bennett Addison ◽  
Takao Kasuga ◽  
Zhiliang Fan
Keyword(s):  
Acid Inhibition ◽  
Cellobiose Dehydrogenase ◽  
Cellobiohydrolase I ◽  
Cellobionic Acid

scholarly journals Characterization of a cellobiose dehydrogenase in the cellulolytic fungus Sporotrichum (Chrysosporium) thermophile

1982 ◽  
Vol 203 (1) ◽  
pp. 277-284 ◽  
Author(s):  
M R Coudray ◽  
G Canevascini ◽  
H Meier
Keyword(s):  
Methylene Blue ◽  
Extracellular Enzyme ◽  
Electron Acceptors ◽  
Cellobiose Dehydrogenase ◽  
Ph Optimum ◽  
Phenazine Methosulphate ◽  
Reduced Activity ◽  
Cellobionic Acid

An extracellular enzyme from culture filtrates of Sporotrichum (Chrysosporium) thermophile (A.T.C.C. 42 464) after growth on cellulose or cellobiose was shown to oxidize cellobiose to cellobionic acid in vitro. Lactose and cellodextrins were also efficiently oxidized, but the enzyme was not active against most mono- and di-saccharides. Several redox substances could act as electron acceptors, but molecular oxygen, tetrazolium salts and NAD(P) were not reduced. Activity was stimulated up to 2-fold in the presence of 0.05 M-Mg2+. The pH optimum of the enzymic reaction was acidic when the activity was tested with dichlorophenol-indophenol or Methylene Blue, but was neutral to alkaline for 3,5-di-t-butyl-1,2-benzoquinone or phenazine methosulphate as electron acceptors. As the enzyme was formed inductively in parallel with the endocellulase, its possible function in relation to cellulolysis is discussed.

Criteria of effective acid inhibition during antihelicobacter pharmacotherapy (according to the findings of express-gastro-pH-monitoring)

2019 ◽  
Vol 0 (3) ◽  
pp. 33-40
Author(s):  
I. G. Paliy ◽  
V. M. Сhernobrovyi ◽  
S. V. Zaika ◽  
I. V. Chernova ◽  
N. M. Kondratiuk
Keyword(s):  
Ph Monitoring ◽  
Acid Inhibition

scholarly journals Fatty Acid Inhibition of Sulfation Factor-stimulated 35SO4 Incorporation into Embryonic Chicken Cartilage

1973 ◽  
Vol 248 (6) ◽  
pp. 1901-1905
Author(s):  
Harry K. Delcher ◽  
George S. Eisenbarth ◽  
Harold E. Lebovitz
Keyword(s):  
Fatty Acid ◽  
Acid Inhibition ◽  
Embryonic Chicken ◽  
Fatty Acid Inhibition

scholarly journals METHIONINE REVERSAL OF 2-CHLORO-4-AMINOBENZOIC ACID INHIBITION IN ESCHERICHIA COLI

1951 ◽  
Vol 188 (2) ◽  
pp. 753-758 ◽  
Author(s):  
C.H. Wang ◽  
Tsoo E. King ◽  
Vernon H. Cheldelin ◽  
Bert E. Christensen
Keyword(s):  
Escherichia Coli ◽  
Acid Inhibition ◽  
Aminobenzoic Acid

scholarly journals Blocking drug efflux mechanisms facilitate genome engineering process in hypercellulolytic fungus, Penicillium funiculosum NCIM1228

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Anmoldeep Randhawa ◽  
Nandita Pasari ◽  
Tulika Sinha ◽  
Mayank Gupta ◽  
Anju M. Nair ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Genome Engineering ◽  
Efflux Pump ◽  
Genetic Manipulation ◽  
Function Analysis ◽  
Drug Tolerance ◽  
Growth Media ◽  
Penicillium Funiculosum ◽  
Cellobiohydrolase I ◽  
Drug Tolerability

Abstract Background Penicillium funiculosum NCIM1228 is a non-model filamentous fungus that produces high-quality secretome for lignocellulosic biomass saccharification. Despite having desirable traits to be an industrial workhorse, P. funiculosum has been underestimated due to a lack of reliable genetic engineering tools. Tolerance towards common fungal antibiotics had been one of the major hindrances towards development of reliable transformation tools against the non-model fungi. In this study, we sought to understand the mechanism of drug tolerance of P. funiculosum and the provision to counter it. We then attempted to identify a robust method of transformation for genome engineering of this fungus. Results Penicillium funiculosum showed a high degree of drug tolerance towards hygromycin, zeocin and nourseothricin, thereby hindering their use as selectable markers to obtain recombinant transformants. Transcriptome analysis suggested a high level expression of efflux pumps belonging to ABC and MFS family, especially when complex carbon was used in growth media. Antibiotic selection medium was optimized using a combination of efflux pump inhibitors and suitable carbon source to prevent drug tolerability. Protoplast-mediated and Agrobacterium-mediated transformation were attempted for identifying efficiencies of linear and circular DNA in performing genetic manipulation. After finding Ti-plasmid-based Agrobacterium-mediated transformation more suitable for P. funiculosum, we improvised the system to achieve random and homologous recombination-based gene integration and deletion, respectively. We found single-copy random integration of the T-DNA cassette and could achieve 60% efficiency in homologous recombination-based gene deletions. A faster, plasmid-free, and protoplast-based CRISPR/Cas9 gene-editing system was also developed for P. funiculosum. To show its utility in P. funiculosum, we deleted the gene coding for the most abundant cellulase Cellobiohydrolase I (CBH1) using a pair of sgRNA directed towards both ends of cbh1 open reading frame. Functional analysis of ∆cbh1 strain revealed its essentiality for the cellulolytic trait of P. funiculosum secretome. Conclusions In this study, we addressed drug tolerability of P. funiculosum and developed an optimized toolkit for its genome modification. Hence, we set the foundation for gene function analysis and further genetic improvements of P. funiculosum using both traditional and advanced methods.

Acid inhibition during anaerobic digestion of biodegradable kitchen waste

2015 ◽  
Vol 7 (2) ◽  
pp. 023118 ◽  
Author(s):  
Benlin Dai ◽  
Jiming Xu ◽  
Yulong He ◽  
Peng Xiong ◽  
Xinfeng Wang ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Acid Inhibition ◽  
Kitchen Waste
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