Pyrroloquinoline quinone modulates the kinetic parameters of the mammalian selenoprotein thioredoxin reductase 1 and is an inhibitor of glutathione reductase

2012 ◽  
Vol 83 (6) ◽  
pp. 815-820 ◽  
Author(s):  
Jianqiang Xu ◽  
Elias S.J. Arnér
Keyword(s):  
Glutathione Reductase ◽  
Kinetic Parameters ◽  
Thioredoxin Reductase ◽  
Pyrroloquinoline Quinone ◽  
Thioredoxin Reductase 1

Truncated mutants of human thioredoxin reductase 1 do not exhibit glutathione reductase activity

FEBS Letters ◽  
2006 ◽  
Vol 580 (15) ◽  
pp. 3595-3600 ◽  
Author(s):  
Sabine Urig ◽  
Johanna Lieske ◽  
Karin Fritz-Wolf ◽  
Angelika Irmler ◽  
Katja Becker
Keyword(s):  
Glutathione Reductase ◽  
Reductase Activity ◽  
Thioredoxin Reductase ◽  
Thioredoxin Reductase 1 ◽  
Glutathione Reductase Activity

scholarly journals Hepatocyte Hyperproliferation upon Liver-Specific Co-disruption of Thioredoxin-1, Thioredoxin Reductase-1, and Glutathione Reductase

Cell Reports ◽  
2017 ◽  
Vol 19 (13) ◽  
pp. 2771-2781 ◽  
Author(s):  
Justin R. Prigge ◽  
Lucia Coppo ◽  
Sebastin S. Martin ◽  
Fernando Ogata ◽  
Colin G. Miller ◽  
...  
Keyword(s):  
Glutathione Reductase ◽  
Thioredoxin Reductase ◽  
Thioredoxin Reductase 1 ◽  
Thioredoxin 1

scholarly journals System-wide identification and prioritization of enzyme substrates by thermal analysis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Amir Ata Saei ◽  
Christian M. Beusch ◽  
Pierre Sabatier ◽  
Juan Astorga Wells ◽  
Hassan Gharibi ◽  
...  
Keyword(s):  
Thermal Analysis ◽  
Thioredoxin Reductase ◽  
Structural Level ◽  
Post Translational Modification ◽  
Post Translational Modifications ◽  
Enzyme Substrate ◽  
Thioredoxin Reductase 1 ◽  
Enzyme Substrates ◽  
Substrate Proteins

AbstractDespite the immense importance of enzyme–substrate reactions, there is a lack of general and unbiased tools for identifying and prioritizing substrate proteins that are modified by the enzyme on the structural level. Here we describe a high-throughput unbiased proteomics method called System-wide Identification and prioritization of Enzyme Substrates by Thermal Analysis (SIESTA). The approach assumes that the enzymatic post-translational modification of substrate proteins is likely to change their thermal stability. In our proof-of-concept studies, SIESTA successfully identifies several known and novel substrate candidates for selenoprotein thioredoxin reductase 1, protein kinase B (AKT1) and poly-(ADP-ribose) polymerase-10 systems. Wider application of SIESTA can enhance our understanding of the role of enzymes in homeostasis and disease, opening opportunities to investigate the effect of post-translational modifications on signal transduction and facilitate drug discovery.

scholarly journals Oxidative stress response in regulatory and conventional T cells: a comparison between patients with chronic coronary syndrome and healthy subjects

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Anna K. Lundberg ◽  
Rosanna W. S. Chung ◽  
Louise Zeijlon ◽  
Gustav Fernström ◽  
Lena Jonasson
Keyword(s):  
Oxidative Stress ◽  
T Cells ◽  
Mononuclear Cells ◽  
Ex Vivo ◽  
Thioredoxin Reductase ◽  
Healthy Controls ◽  
Antioxidant Genes ◽  
Consistent Finding ◽  
Coronary Syndrome ◽  
Thioredoxin Reductase 1

Abstract Background Inflammation and oxidative stress form a vicious circle in atherosclerosis. Oxidative stress can have detrimental effects on T cells. A unique subset of CD4+ T cells, known as regulatory T (Treg) cells, has been associated with atheroprotective effects. Reduced numbers of Treg cells is a consistent finding in patients with chronic coronary syndrome (CCS). However, it is unclear to what extent these cells are sensitive to oxidative stress. In this pilot study, we tested the hypothesis that oxidative stress might be a potential contributor to the Treg cell deficit in CCS patients. Methods Thirty patients with CCS and 24 healthy controls were included. Treg (CD4+CD25+CD127−) and conventional T (CD4+CD25−, Tconv) cells were isolated and treated with increasing doses of H2O2. Intracellular ROS levels and cell death were measured after 2 and 18 h, respectively. The expression of antioxidant genes was measured in freshly isolated Treg and Tconv cells. Also, total antioxidant capacity (TAC) was measured in fresh peripheral blood mononuclear cells, and oxidized (ox) LDL/LDL ratios were determined in plasma. Results At all doses of H2O2, Treg cells accumulated more ROS and exhibited higher rates of death than their Tconv counterparts, p < 0.0001. Treg cells also expressed higher levels of antioxidant genes, including thioredoxin and thioredoxin reductase-1 (p < 0.0001), though without any differences between CCS patients and controls. Tconv cells from CCS patients were, on the other hand, more sensitive to oxidative stress ex vivo and expressed more thioredoxin reductase-1 than Tconv cells from controls, p < 0.05. Also, TAC levels were lower in patients, 0.97 vs 1.53 UAE/100 µg, p = 0.001, while oxLDL/LDL ratios were higher, 29 vs 22, p = 0.006. Conclusion Treg cells isolated from either CCS patients or healthy controls were all highly sensitive to oxidative stress ex vivo. There were signs of oxidant-antioxidant imbalance in CCS patients and we thus assume that oxidative stress may play a role in the reduction of Treg cells in vivo.

Inhibition of Both Thioredoxin Reductase and Glutathione Reductase may Contribute to the Anticancer Mechanism of TH-302

2009 ◽  
Vol 136 (3) ◽  
pp. 294-301 ◽  
Author(s):  
Shengrong Li ◽  
Jinsong Zhang ◽  
Jun Li ◽  
Dongming Chen ◽  
Mark Matteucci ◽  
...  
Keyword(s):  
Glutathione Reductase ◽  
Thioredoxin Reductase ◽  
Anticancer Mechanism

scholarly journals The Core Promoter of Human Thioredoxin Reductase 1

2001 ◽  
Vol 276 (32) ◽  
pp. 30542-30551 ◽  
Author(s):  
Anna-Klara Rundlöf ◽  
Mattias Carlsten ◽  
Elias S. J. Arnér
Keyword(s):  
Core Promoter ◽  
Thioredoxin Reductase ◽  
The Core ◽  
Thioredoxin Reductase 1

Thioredoxin Reductase 1 Modulates Pigmentation and Photobiology of Murine Melanocytes in vivo

2022 ◽  
Author(s):  
Evan L. Carpenter ◽  
Mark B. Wyant ◽  
Aaryan Indra ◽  
Shosuke Ito ◽  
Kazumasa Wakamatsu ◽  
...  
Keyword(s):  
Thioredoxin Reductase ◽  
Thioredoxin Reductase 1
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