Pharmacological characterization of protease activated receptor-1 by a serum responsive element-dependent reporter gene assay: Major role of calmodulin

2006 ◽  
Vol 71 (10) ◽  
pp. 1449-1458 ◽  
Author(s):  
Luc De Vries ◽  
Christiane Palmier ◽  
Frederic Finana ◽  
Bruno Le Grand ◽  
Michel Perez ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Reporter Gene Assay ◽  
Pharmacological Characterization ◽  
Gene Assay ◽  
Protease Activated Receptor 1 ◽  
Responsive Element

Combining an in vitro reporter gene assay with metabolomics to identify tomato phytochemicals responsible for inducing electrophile-responsive element (EpRE)-mediated gene transcription

Metabolomics ◽  
2014 ◽  
Vol 11 (2) ◽  
pp. 302-311 ◽  
Author(s):  
Henriëtte D. L. M. van Eekelen ◽  
Linda Gijsbers ◽  
Chris A. Maliepaard ◽  
Robert A. M. Vreeburg ◽  
Richard Finkers ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Gene Transcription ◽  
Reporter Gene Assay ◽  
Gene Assay ◽  
Responsive Element

Unbiased compound screening with a reporter gene assay highlights the role of p13 in the cardiac cellular stress response

2018 ◽  
Vol 495 (2) ◽  
pp. 1992-1997
Author(s):  
Naoki Inoue ◽  
Taisei Hirouchi ◽  
Atsushi Kasai ◽  
Shintaro Higashi ◽  
Natsumi Hiraki ◽  
...  
Keyword(s):  
Stress Response ◽  
Reporter Gene ◽  
Cellular Stress ◽  
Reporter Gene Assay ◽  
Cellular Stress Response ◽  
Gene Assay ◽  
Compound Screening

scholarly journals LINC01213 promotes prostate cancer cell progression through miR-597/ BCL2L1 axis

2021 ◽  
Author(s):  
Xian Zhao ◽  
Xiaojing Xu ◽  
Qiong Wang ◽  
Xiaofei Wu
Keyword(s):  
Prostate Cancer ◽  
Reporter Gene ◽  
Reporter Gene Assay ◽  
Luciferase Reporter ◽  
Luciferase Reporter Gene ◽  
Western Blot Assay ◽  
Luciferase Reporter Gene Assay ◽  
Gene Assay

Abstract Background: Majority of cancer related deaths in males are attributed to prostate cancer (PRAD) throughout the world. Recently, the role of long non-coding RNAs (lncRNAs) in the pathogenesis of cancer has been widely explored. In this study, we investigated the role of lncRNA LINC01213 (LINC01213) in tumorigenesis of prostate cancer (PRAD).Methods: PRAD and adjacent tissue samples were collected from cancer patients. Survival rate among these patients was compared by Kaplan–Meier analysis. PRAD cells viability was estimated by CCK-8 method while AnnexinV/PI cytometry assay was used to determine the percent of apoptotic cells. qRT-PCR and western blot assay were used to determine the mRNA and protein expressions, respectively. Interaction between LINC01213 and corresponding miRNA as well as between miRNA and mRNA was confirmed by dual luciferase reporter gene assay. PRAD cells were also injected subcutaneously in nude mice to support in vitro findings.Results: It was observed that LINC01213 was highly expressed in PRAD samples and cell lines. Down-regulation of LINC01213 in PRAD cells decreased cell viability and inhibited proliferation. Luciferase reporter gene assay and RNA pull-down confirmed that LINC01213 targeted miR-597-3p. Increased expression of miR-597-3p resulted in decreased BCL2L2 expression in vitro. Inhibitory effects of miR-597-3p on PRAD cells’ survival and growth were diminished after LINC01213 overexpression which was also associated with alteration in the protein expression of BCL-xL, BCL-2 as well as caspase 3 and caspase 9.Conclusion: Taken together, our findings suggest that LINC01213 plays its role in PRAD tumorigenesis through miR-597-3p/ BCL2L2 dependent pathway with associated modulation of genes involved in cell survival and apoptosis.

Identification and characterization of human interferon alpha inhibitors through a WISH cell line-based reporter gene assay

2020 ◽  
Vol 94 ◽  
pp. 103372
Author(s):  
Milagros Bürgi ◽  
Paola Hernández ◽  
Mauricio Cabrera ◽  
Hugo Cerecetto ◽  
Mercedes González ◽  
...  
Keyword(s):  
Cell Line ◽  
Reporter Gene ◽  
Interferon Alpha ◽  
Reporter Gene Assay ◽  
Human Interferon ◽  
Gene Assay ◽  
Identification And Characterization

Microbial reporter gene assay as a diagnostic and early warning tool for the detection and characterization of toxic pollution in surface waters

2015 ◽  
Vol 34 (11) ◽  
pp. 2523-2532 ◽  
Author(s):  
Christine Hug ◽  
Xiaowei Zhang ◽  
Miao Guan ◽  
Martin Krauss ◽  
Robert Bloch ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Surface Waters ◽  
Early Warning ◽  
Reporter Gene Assay ◽  
Toxic Pollution ◽  
Gene Assay

Characterization of neutralizing antibodies to interferon αλπηα using a novel reporter-gene assay

Cytokine ◽  
2009 ◽  
Vol 48 (1-2) ◽  
pp. 93
Author(s):  
Brigitte Blanchard ◽  
Christophe Lallemand ◽  
Jean-François Meritet ◽  
Pierre Lebon ◽  
Michael G. Tovey
Keyword(s):  
Reporter Gene ◽  
Neutralizing Antibodies ◽  
Reporter Gene Assay ◽  
Gene Assay

scholarly journals In vitro bioanalytical assessment of toxicity of wetland samples from Spanish Mediterranean coastline

2021 ◽  
Vol 33 (1) ◽  
Author(s):  
Alberto Celma ◽  
Geeta Mandava ◽  
Agneta Oskarsson ◽  
Juan Vicente Sancho ◽  
Lubertus Bijlsma ◽  
...  
Keyword(s):  
Water Quality ◽  
Reporter Gene ◽  
Biological Activities ◽  
Reporter Gene Assay ◽  
Water Bodies ◽  
Good Tool ◽  
Adverse Health Effects ◽  
Aryl Hydrocarbon ◽  
Gene Assay

Abstract Background Fresh water bodies represent less than 1% of overall amount of water on earth and ensuring their quality and sustainability is pivotal. Although several campaigns have been performed to monitor the occurrence of micropollutants by means of chemical analysis, this might not cover the whole set of chemicals present in the sample nor the potential toxic effects of mixtures of natural and anthropogenic chemicals. In this sense, by selecting relevant toxicity endpoints when performing in vitro bioanalysis, effect-based methodologies can be of help to perform a comprehensive assessment of water quality and reveal biological activities relevant to adverse health effects. However, no prior bioanalytical study was performed in wetland water samples from the Spanish Mediterranean coastline. Methods Eleven samples from relevant water bodies from the Spanish Mediterranean coastline were collected to monitor water quality on 8 toxicity endpoints. Aryl hydrocarbon receptor (AhR), androgenicity (AR+ and AR−), estrogenicity (ER+ and ER−), oxidative stress response (Nrf2) and vitamin D receptor (VDR+ and VDR−) reporter gene assays were evaluated. Results AhR was the reporter gene assay showing a more frequent response over the set of samples (activated by 9 out of 11 samples), with TCDD-eq in the range 7.7–22.2 pM. For AR, ER and VDR assays sporadic activations were observed. Moreover, no activity was observed on the Nrf2 reporter gene assay. Wastewater and street runaway streams from Valencia could be responsible for enhanced activities in one of the water inputs in the Natural Park ‘L’Albufera’. Conclusions Water quality of relevant wetlands from the Spanish Mediterranean coastline has been evaluated. The utilization of a panel of 5 different bioassays to cover for different toxicity endpoints has demonstrated to be a good tool to assess water quality.

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