Eupatilin, a pharmacologically active flavone derived from Artemisia plants, induces cell cycle arrest in ras-transformed human mammary epithelial cells

2004 ◽  
Vol 68 (6) ◽  
pp. 1081-1087 ◽  
Author(s):  
Do-Hee Kim ◽  
Hye-Kyung Na ◽  
Tae Young Oh ◽  
Won-Bae Kim ◽  
Young-Joon Surh
Keyword(s):  
Cell Cycle ◽  
Epithelial Cells ◽  
Cell Cycle Arrest ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Human Mammary Epithelial Cells ◽  
Cycle Arrest ◽  
Human Mammary Epithelial ◽  
Pharmacologically Active

scholarly journals The MicroRNA 424/503 Cluster Reduces CDC25A Expression during Cell Cycle Arrest Imposed by Transforming Growth Factor   in Mammary Epithelial Cells

2014 ◽  
Vol 34 (23) ◽  
pp. 4216-4231 ◽  
Author(s):  
D. Llobet-Navas ◽  
R. Rodriguez-Barrueco ◽  
J. de la Iglesia-Vicente ◽  
M. Olivan ◽  
V. Castro ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Growth Factor ◽  
Epithelial Cells ◽  
Cell Cycle Arrest ◽  
Transforming Growth Factor ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Cycle Arrest

Signaling and apoptosis differences between severe hypoxia and desferoxamine treatment of human epithelial cells

2008 ◽  
Vol 86 (5) ◽  
pp. 425-436 ◽  
Author(s):  
Adrian Harold Box ◽  
Carol Yuen ◽  
Dragana Ponjevic ◽  
Gordon H. Fick ◽  
Douglas James Demetrick
Keyword(s):  
Cell Cycle ◽  
Cell Death ◽  
Epithelial Cells ◽  
Cell Cycle Arrest ◽  
Cell Lines ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Severe Hypoxia ◽  
Cycle Arrest ◽  
Induced Apoptosis

The mechanisms by which cells undergo proliferation arrest or cell death in response to hypoxia are still not completely understood. Originally, we showed that HeLa and Hep3B carcinoma cells undergo different proliferation responses in hypoxia. We now show that these 2 cell lines also have different cell death responses to severe hypoxia, with HeLa showing both cell cycle arrest and apoptosis (as early as 12 h after hypoxia treatment), and Hep3B showing resistance to both. Hypoxia-induced apoptosis in Hela was associated with decreases of both phospho-S473- and -T308-AKT and loss of AKT function, whereas Hep3B cells were resistant to hypoxia-induced apoptosis and did not lose phospho-AKT or AKT function. We then decided to test if our observations were confirmed using a hypoxia mimic, desferoxamine. Desferoxamine treatment yielded cell cycle arrest in HeLa and moderate arrest in Hep3B but, surprisingly, did not induce notable apoptosis of either cell line with up to 24 h of treatment. Hypoxia-treated normal human mammary epithelial cells also showed hypoxia-induced apoptosis. Interestingly, in these cell lines, there was a complete correlation between loss of phospho-AKT and (or) total AKT, and susceptibility to hypoxia-induced apoptosis. Our data suggests a model in which regulated loss of active AKT at a precise time point in hypoxia may be associated with apoptosis in susceptible cells.

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