scholarly journals Angiopoietin-like-2 and -3 act through their coiled-coil domains to enhance survival and replating capacity of human cord blood hematopoietic progenitors

2012 ◽  
Vol 48 (1) ◽  
pp. 25-29 ◽  
Author(s):  
Hal E. Broxmeyer ◽  
Edward F. Srour ◽  
Scott Cooper ◽  
Carrie T. Wallace ◽  
Giao Hangoc ◽  
...  
Keyword(s):  
Cord Blood ◽  
Coiled Coil ◽  
Hematopoietic Progenitors ◽  
Human Cord Blood

scholarly journals Identification of a Hierarchy of Multipotent Hematopoietic Progenitors in Human Cord Blood

2007 ◽  
Vol 1 (6) ◽  
pp. 635-645 ◽  
Author(s):  
Ravindra Majeti ◽  
Christopher Y. Park ◽  
Irving L. Weissman
Keyword(s):  
Cord Blood ◽  
Hematopoietic Progenitors ◽  
Human Cord Blood

The AC133+CD38−, but not the rhodamine-low, phenotype tracks LTC-IC and SRC function in human cord blood ex vivo expansion cultures

Blood ◽  
2010 ◽  
Vol 115 (2) ◽  
pp. 257-260 ◽  
Author(s):  
Caryn Y. Ito ◽  
Daniel C. Kirouac ◽  
Gerard J. Madlambayan ◽  
Mei Yu ◽  
Ian Rogers ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
Ex Vivo ◽  
Hematopoietic Progenitors ◽  
Human Cord Blood ◽  
Hematopoietic Stem ◽  
Expansion System ◽  
And Function ◽  
Limiting Dilution

Abstract Phenotypic markers associated with human hematopoietic stem cells (HSCs) were developed and validated using uncultured cells. Because phenotype and function can be dissociated during culture, better markers to prospectively track and isolate HSCs in ex vivo cultures could be instrumental in advancing HSC-based therapies. Using an expansion system previously shown to increase hematopoietic progenitors and SCID-repopulating cells (SRCs), we demonstrated that the rhodamine-low phenotype was lost, whereas AC133 expression was retained throughout culture. Furthermore, the AC133+CD38− subpopulation was significantly enriched in long-term culture-initiating cells (LTC-IC) and SRCs after culture. Preculture and postculture analysis of total nucleated cell and LTC-IC number, and limiting dilution analysis in NOD/SCID mice, showed a 43-fold expansion of the AC133+CD38− subpopulation that corresponded to a 7.3-fold and 4.4-fold expansion of LTC-ICs and SRCs in this subpopulation, respectively. Thus, AC133+CD38− is an improved marker that tracks and enriches for LTC-IC and SRC in ex vivo cultures.

scholarly journals Thrombopoietin augments ex vivo expansion of human cord blood-derived hematopoietic progenitors in combination with stem cell factor and flt3 ligand

Leukemia ◽  
1997 ◽  
Vol 11 (4) ◽  
pp. 524-530 ◽  
Author(s):  
Y Ohmizono ◽  
H Sakabe ◽  
T Kimura ◽  
S Tanimukai ◽  
T Matsumura ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cord Blood ◽  
Stem Cell Factor ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Flt3 Ligand ◽  
Hematopoietic Progenitors ◽  
Human Cord Blood
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