Multiplex primer extension reaction screening and oxidative challenge of glucose-6-phosphate dehydrogenase mutants in hemizygous and heterozygous subjects

2006 ◽  
Vol 37 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Chun Hay Ko ◽  
Edmund Yung ◽  
Karen Li ◽  
Chung Leung Li ◽  
Pak Cheung Ng ◽  
...  
Keyword(s):  
Primer Extension ◽  
Primer Extension Reaction ◽  
Oxidative Challenge ◽  
Glucose 6 Phosphate Dehydrogenase

Multi-allele DNA biosensor for the rapid genotyping of ‘nondeletion’ alpha thalassaemia mutations in HBA1 and HBA2 genes by means of multiplex primer extension reaction

2015 ◽  
Vol 446 ◽  
pp. 241-247 ◽  
Author(s):  
Margarita Petropoulou ◽  
Amalia Poula ◽  
Jan Traeger-Synodinos ◽  
Emmanuel Kanavakis ◽  
Theodore K. Christopoulos ◽  
...  
Keyword(s):  
Dna Biosensor ◽  
Primer Extension ◽  
Primer Extension Reaction ◽  
Alpha Thalassaemia

scholarly journals Mutant T7 RNA polymerase is capable of catalyzing DNA primer extension reaction

FEBS Letters ◽  
1998 ◽  
Vol 423 (2) ◽  
pp. 189-192 ◽  
Author(s):  
E.E Rusakova ◽  
V.L Tunitskaya ◽  
L.V Memelova ◽  
S.V Kochetkova ◽  
D.A Kostyuk ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Primer Extension ◽  
T7 Rna Polymerase ◽  
Primer Extension Reaction

scholarly journals Oxidative Stress Evokes a Metabolic Adaptation That Favors Increased NADPH Synthesis and Decreased NADH Production in Pseudomonas fluorescens

2007 ◽  
Vol 189 (18) ◽  
pp. 6665-6675 ◽  
Author(s):  
Ranji Singh ◽  
Ryan J. Mailloux ◽  
Simone Puiseux-Dao ◽  
Vasu D. Appanna
Keyword(s):  
Oxidative Stress ◽  
Metabolic Pathways ◽  
Metabolic Networks ◽  
Tricarboxylic Acid Cycle ◽  
Tricarboxylic Acid ◽  
Metabolic Adaptation ◽  
Atp Production ◽  
Oxidative Challenge ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Oxidative Insult

ABSTRACT The fate of all aerobic organisms is dependent on the varying intracellular concentrations of NADH and NADPH. The former is the primary ingredient that fuels ATP production via oxidative phosphorylation, while the latter helps maintain the reductive environment necessary for this process and other cellular activities. In this study we demonstrate a metabolic network promoting NADPH production and limiting NADH synthesis as a consequence of an oxidative insult. The activity and expression of glucose-6-phosphate dehydrogenase, malic enzyme, and NADP+-isocitrate dehydrogenase, the main generators of NADPH, were markedly increased during oxidative challenge. On the other hand, numerous tricarboxylic acid cycle enzymes that supply the bulk of intracellular NADH were significantly downregulated. These metabolic pathways were further modulated by NAD+ kinase (NADK) and NADP+ phosphatase (NADPase), enzymes known to regulate the levels of NAD+ and NADP+. While in menadione-challenged cells, the former enzyme was upregulated, the phosphatase activity was markedly increased in control cells. Thus, NADK and NADPase play a pivotal role in controlling the cross talk between metabolic networks that produce NADH and NADPH and are integral components of the mechanism involved in fending off oxidative stress.

Primer Extension Reaction Assays for Incorporation of Deoxynucleotide Analogue into DNA

2015 ◽  
Vol 33 (2) ◽  
pp. 192-198
Author(s):  
Xilong Wang ◽  
Xun Chen ◽  
Yonghui Liu ◽  
Jin Zhu
Keyword(s):  
Primer Extension ◽  
Primer Extension Reaction
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