scholarly journals Functional study of transcription factor KLF11 by targeted gene inactivation

2005 ◽  
Vol 34 (1) ◽  
pp. 53-59 ◽  
Author(s):  
C SONG ◽  
G GAVRIILIDIS ◽  
H ASANO ◽  
G STAMATOYANNOPOULOS
Keyword(s):  
Transcription Factor ◽  
Gene Inactivation ◽  
Functional Study ◽  
Targeted Gene Inactivation

Dissecting Adrenal and Behavioral Responses to Stress by Targeted Gene Inactivation in Mice

Stress ◽  
2003 ◽  
Vol 6 (2) ◽  
pp. 121-125 ◽  
Author(s):  
Judson A. Brewer ◽  
Kathleen E. Bethin ◽  
Michele L. Schaefer ◽  
Lisa M. Muglia ◽  
Sherri K. Vogt ◽  
...  
Keyword(s):  
Behavioral Responses ◽  
Gene Inactivation ◽  
Targeted Gene Inactivation

A Role for the Wilms’ Tumor Protein WT1 in Organ Development

Physiology ◽  
2005 ◽  
Vol 20 (1) ◽  
pp. 54-59 ◽  
Author(s):  
Holger Scholz ◽  
Karin M. Kirschner
Keyword(s):  
Tumor Suppressor ◽  
Tumor Suppressor Gene ◽  
Wilms Tumor ◽  
Suppressor Gene ◽  
Gene Inactivation ◽  
Organ Development ◽  
Genitourinary System ◽  
Wilms Tumor 1 ◽  
Targeted Gene Inactivation

Wilms’ tumor (nephroblastoma) represents a unique example of an aberrant kidney formation that can result from mutations in a tumor suppressor gene, Wilms’ tumor 1 ( WT1). Targeted gene inactivation in mice testifies that WT1 is a master switch for the development of the genitourinary system and other organs.

scholarly journals Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases

2008 ◽  
Vol 26 (6) ◽  
pp. 695-701 ◽  
Author(s):  
Xiangdong Meng ◽  
Marcus B Noyes ◽  
Lihua J Zhu ◽  
Nathan D Lawson ◽  
Scot A Wolfe
Keyword(s):  
Zinc Finger ◽  
Gene Inactivation ◽  
Zinc Finger Nucleases ◽  
Targeted Gene Inactivation

scholarly journals Liver-specific Gene Inactivation of the Transcription Factor ATF4 Alleviates Alcoholic Liver Steatosis in Mice

2016 ◽  
Vol 291 (35) ◽  
pp. 18536-18546 ◽  
Author(s):  
Kai Li ◽  
Yuzhong Xiao ◽  
Junjie Yu ◽  
Tingting Xia ◽  
Bin Liu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Liver Steatosis ◽  
Gene Inactivation ◽  
Specific Gene

Targeted gene inactivation for the elucidation of deoxysugar biosynthesis in the erythromycin producer Saccharopolyspora erythraea

1998 ◽  
Vol 257 (5) ◽  
pp. 542-553 ◽  
Author(s):  
K. Salah-Bey ◽  
M. Doumith ◽  
J.-M. Michel ◽  
S. Haydock ◽  
J. Cortés ◽  
...  
Keyword(s):  
Gene Inactivation ◽  
Saccharopolyspora Erythraea ◽  
Targeted Gene Inactivation

scholarly journals Characterization of Tomato Transcription Factor WUSCHEL and Functional Study in Arabidopsis

2012 ◽  
Vol 11 (8) ◽  
pp. 1257-1265 ◽  
Author(s):  
Xiang WANG ◽  
Xin-guo WANG ◽  
Jiang-ping REN ◽  
Ying MA ◽  
Jun YIN
Keyword(s):  
Transcription Factor ◽  
Functional Study

scholarly journals Targeted gene inactivation reveals a functional role of calpain-1 in platelet spreading

2012 ◽  
Vol 10 (6) ◽  
pp. 1120-1132 ◽  
Author(s):  
S. M. KUCHAY ◽  
A. J. WIESCHHAUS ◽  
M. MARINKOVIC ◽  
I. M. HERMAN ◽  
A. H. CHISHTI
Keyword(s):  
Functional Role ◽  
Gene Inactivation ◽  
Targeted Gene Inactivation ◽  
Platelet Spreading

scholarly journals Genome-Wide DNA Methylation Analysis of Soybean Curled-Cotyledons Mutant and Functional Evaluation of a Homeodomain-Leucine Zipper (HD-Zip) I Gene GmHDZ20

2021 ◽  
Vol 11 ◽  
Author(s):  
Hui Yang ◽  
Zhongyi Yang ◽  
Zhuozhuo Mao ◽  
Yali Li ◽  
Dezhou Hu ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Transcription Factor ◽  
Leucine Zipper ◽  
Functional Study ◽  
Functional Evaluation ◽  
Whole Genome ◽  
Ontology Term ◽  
Genome Wide ◽  
A Genome ◽  
Differentially Methylated Genes

DNA methylation is a major, conserved epigenetic modification that influences many biological processes. Cotyledons are specialized tissues that provide nutrition for seedlings at the early developmental stage. To investigate the patterns of genomic DNA methylation of germinated cotyledons in soybean (Glycine max) and its effect on cotyledon development, we performed a genome-wide comparative analysis of DNA methylation between the soybean curled-cotyledons (cco) mutant, which has abnormal cotyledons, and its corresponding wild type (WT) by whole-genome bisulfite sequencing. The cco mutant was methylated at more sites but at a slightly lower level overall than the WT on the whole-genome level. A total of 46 CG-, 92 CHG-, and 9723 CHH- (H = A, C, or T) differentially methylated genes (DMGs) were identified in cotyledons. Notably, hypomethylated CHH-DMGs were enriched in the gene ontology term “sequence-specific DNA binding transcription factor activity.” We selected a DMG encoding a homeodomain-leucine zipper (HD-Zip) I subgroup transcription factor (GmHDZ20) for further functional characterization. GmHDZ20 localized to the nucleus and was highly expressed in leaf and cotyledon tissues. Constitutive expression of GmHDZ20 in Arabidopsis thaliana led to serrated rosette leaves, shorter siliques, and reduced seed number per silique. A yeast two-hybrid assay revealed that GmHDZ20 physically interacted with three proteins associated with multiple aspects of plant growth. Collectively, our results provide a comprehensive study of soybean DNA methylation in normal and aberrant cotyledons, which will be useful for the identification of specific DMGs that participate in cotyledon development, and also provide a foundation for future in-depth functional study of GmHDZ20 in soybean.

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