In vitro determination of prebiotic potential of aqueous extract of horse chestnut by-product

2019 ◽  
Vol 19 ◽  
pp. 100190
Author(s):  
Joanna Przyborska ◽  
Michael C. Hall ◽  
Mary Concannon
Keyword(s):  
Aqueous Extract ◽  
Horse Chestnut

AN ACCURATE METHOD FOR THE ESTIMATION OF LOW CONCENTRATIONS OF DEXTRAN IN PLASMA

1957 ◽  
Vol 35 (6) ◽  
pp. 383-390 ◽  
Author(s):  
Robert E. Semple
Keyword(s):  
Aqueous Extract ◽  
Accurate Method ◽  
Protein Precipitation ◽  
In Vitro Tests ◽  
Plasma Samples ◽  
Carbohydrate Concentration ◽  
Low Concentrations ◽  
Standard Deviations

A method is presented for the determination in plasma of small (50–150 mg./100 ml.) amounts of dextran. The procedure, which requires between 3 and 4 hours, consists of protein precipitation, glucose removal by dialysis, and the determination of the carbohydrate concentration of the resulting aqueous extract by a modified anthrone technique. Results of in vitro tests show that average dextran recovery is essentially 100% and that standard deviations in recovery range from 1.7 to 2.5% depending upon the dextran concentration. Deviations are reduced to a range of 1.4–1.7% by the use of duplicate plasma samples.

scholarly journals In vitro Anti-Arthritic activity of Pseudarthria viscida against Protein Denaturation and Proteinase enzyme

2020 ◽  
Vol 11 (2) ◽  
pp. 284-288
Author(s):  
Pandian P
Keyword(s):  
Enzyme Activity ◽  
Aqueous Extract ◽  
Protein Denaturation ◽  
Bone Erosion ◽  
Diclofenac Sodium ◽  
Ethanol Extract ◽  
Maximum Inhibition

Arthritis is an autoimmune with chronic inflammatory, the patient has very painful due deformities and bone erosion which is caused by damage of the joints. The plant Pseudarthria viscida was collected from the Thirunelveli district and extracted with aqueous and ethanol solvent. The two method was used for determination of invitro anti-arthritic activity. The Inhibition of Protein Denaturation Method shows the anti-arthritic activity with the value from 40.46±0.72 to 78.36±0.64 for aqueous extract and 48.62±0.86 to 84.42±0.86 for ethanol extract and Inhibition of Proteinase Enzyme Activity shows 38.62±0.32 to 72.58±0.58 in aqueous extract and 46.28±0.58 to 80.52±0.56 in ethanol extract. Diclofenac sodium were used as standard, the concentration is 100, 200, 300, 400, and 500. In both the method the concentration of 500Microgram per milliliters shows maximum inhibition and compare to both extract the ethanol shows better activity than aqueous extract.

scholarly journals Chemical profile and potential antifungal of essential oil Schinus terebinthifolius and its by-products

2020 ◽  
Vol 9 (11) ◽  
pp. e91491110623
Author(s):  
Luiz Fernando Ganassali de Oliveira Júnior ◽  
Alexandre Passos Oliveira ◽  
Pryanka Thuyra Nascimento Fontes ◽  
Lucas Fonseca Menezes de Oliveira ◽  
Marília Cavalcante Santos ◽  
...  
Keyword(s):  
Essential Oil ◽  
Aqueous Extract ◽  
Steam Distillation ◽  
Chemical Constituents ◽  
Chemical Compounds ◽  
Schinus Terebinthifolius ◽  
Vitro Development ◽  
By Products

This study aimed to investigate the distillation time to obtain a higher yield of essential oil and by-product (extract and hydrolate) of Schinus terebinthifolius seeds, to determine the main chemical constituents of these products and the potential fungitoxic on the Colletotrichum gloeosporioides. To obtain the essential oil and by-products, we used the method of steam distillation for 2.5, 4.0, 5.5 and 7.0 hours. The determination of the chemical composition was made by GC/MS. To determine the potential fungitoxic on mycelial growth of C. gloeosporioides various concentrations of the oil and by-products were tested. Distillation periods did not influence the yield of aqueous extract and essential oil hydrolate. No chemical compounds have been identified in hydrolat or aqueous extract with the use of GC analysis. The essential oil at concentrations of 2%, 3% and 4% inhibited approximately 47% of the in vitro development of the fungus C. gloeosporioides.

scholarly journals Determination of the antimutagenicity of an aqueous extract of Rhizophora mangle L. (Rhizophoraceae), using in vivo and in vitro test systems

2009 ◽  
Vol 33 (1) ◽  
pp. 176-181 ◽  
Author(s):  
Maressa Malini ◽  
Maria Aparecida Marin-Morales ◽  
Mário Sérgio Mantovani ◽  
Claudia Masrouah Jamal ◽  
Natália Nati ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Rhizophora Mangle ◽  
In Vitro Test ◽  
Test Systems ◽  
Vitro Test

scholarly journals Evaluation of the Antibacterial and Antifungal Properties of Phragmanthera capitata (Sprengel) Balle (Loranthaceae), a Mistletoe Growing on Rubber Tree, Using the Dilution Techniques

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Franklin Uangbaoje Ohikhena ◽  
Olubunmi Abosede Wintola ◽  
Anthony Jide Afolayan
Keyword(s):  
Aqueous Extract ◽  
Rubber Tree ◽  
Antimicrobial Properties ◽  
Bactericidal Effect ◽  
Antimicrobial Activities ◽  
Gram Positive Bacteria ◽  
Agar Dilution ◽  
Antibacterial And Antifungal

The alarming increase in multidrug resistance of pathogenic microorganisms to conventional drugs in recent years has prompted the search for new leads in alternative remedies in natural products. Hence, this study was aimed at evaluating the antimicrobial properties of Phragmanthera capitata, a parasitic mistletoe growing on rubber trees. The in vitro antimicrobial activities of the acetone, methanol, ethanol, and aqueous extracts were investigated using five gram-negative and five gram-positive bacteria and four fungi. A 96-well resazurin broth and agar dilution techniques were used for the determination of the Minimum Inhibitory and Bactericidal Concentrations. The antibacterial activity of the organic extracts had comparative effects on all the bacteria with a MIC of 1.25 to 5 mg/mL and MBC of 2.5 to 10 mg/mL. However, the acetone extract showed higher bactericidal effect while the aqueous extract was not active. The organic solvent extracts also showed antifungal activities on two of the fungi with a MIC of 1.25 mg/mL to 10 mg/mL. However, the aqueous extract had the highest activity inhibiting all the fungi with a MIC of ≤0.3125 to 1.25 mg/mL. The study supports the ethnomedicinal claims of P. capitata as a remedy for the diseases/infections caused by these organisms.

scholarly journals Evaluation of the Antioxidant Activity of Stalk and Fruit of Solanum aethiopicum L. (Solanaceae)

2020 ◽  
pp. 6-12
Author(s):  
Kady Diatta ◽  
William Diatta ◽  
Alioune Dior Fall ◽  
Serigne Ibra Mbacké Dieng ◽  
Amadou Ibrahima Mbaye ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Reducing Power ◽  
Ethanol Extract ◽  
New Drugs ◽  
Solanum Aethiopicum ◽  
Dpph Method ◽  
The Many

Background: The use of plants for healing dates back to very remote times. Nowadays with the accession of new diseases plants are increasingly used for the formulation of new drugs able to overcome the many diseases (cancer, atherosclerosis) often caused by the disorder of the system prooxidant/antioxidant. Aim/Objective: On the strength of this observation, the research of an antioxidant plant is essential, hence the aim of this study, which is to determine the antioxidant activity of the stalk and the fruit of Solanum aethiopicum L. Methods: The fruits and stalk were washed, cut into fine slats, then dried in the incubator for three days and finally crushed into powder. An extraction by decoction with ethanol (stalks and fruits) and water (fruit) was subsequently carried out to obtain three extracts (ethanol and water). Antioxidant activity was evaluated through the FRAP method, and the trapping of radical DPPH. Results: For the FRAP method, at the highest concentration (1 mg/ml) the aqueous extract of the fruit (74.84±2.97%) has a higher reducing power compared to those of the ethanolic extracts of the fruit (70.15 ± 5.72%) and the stalk (49.85 ± 2.11%). These reducing powers, although significant, remain lower than those of tannic acid (89.95±0.007%). And finally, for the DPPH method, the aqueous extract of the fruit is more effective in reducing free radical DPPH with a IC50= 162±33 µg/ml, follow up by ethanol extract from the stalk (IC50= 360± 90 µg/ml) and finally ethanol extract from the fruit (IC50= 362.5± 23.5 µg/ml). These results confirm the in vitro antioxidant activity of the studied parts of Solanum aethiopicum. Conclusion: Prospective studies could focus on acute and subacute toxicities and the determination of the molecules responsible for the activity.

AN ACCURATE METHOD FOR THE ESTIMATION OF LOW CONCENTRATIONS OF DEXTRAN IN PLASMA

1957 ◽  
Vol 35 (1) ◽  
pp. 383-390
Author(s):  
Robert E. Semple
Keyword(s):  
Aqueous Extract ◽  
Accurate Method ◽  
Protein Precipitation ◽  
In Vitro Tests ◽  
Plasma Samples ◽  
Carbohydrate Concentration ◽  
Low Concentrations ◽  
Standard Deviations

A method is presented for the determination in plasma of small (50–150 mg./100 ml.) amounts of dextran. The procedure, which requires between 3 and 4 hours, consists of protein precipitation, glucose removal by dialysis, and the determination of the carbohydrate concentration of the resulting aqueous extract by a modified anthrone technique. Results of in vitro tests show that average dextran recovery is essentially 100% and that standard deviations in recovery range from 1.7 to 2.5% depending upon the dextran concentration. Deviations are reduced to a range of 1.4–1.7% by the use of duplicate plasma samples.

Determination of in vitro „anti-aging„-effects of Ganoderma pfeifferi

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
W Jülich ◽  
J Pörksen ◽  
H Welzel ◽  
U Lindequist
Keyword(s):  
Aging Effects
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