Development of plantlet regeneration pathway using in vitro leaf of Ficus carica L. cv. Panachee supported with histological analysis

2020 ◽  
Vol 27 ◽  
pp. 101697
Author(s):  
Eyu Chan Hong ◽  
Chew Bee Lynn ◽  
Sreeramanan Subramaniam
Keyword(s):  
Histological Analysis ◽  
Plantlet Regeneration ◽  
Ficus Carica ◽  
Regeneration Pathway

Patterns of pigmentation in experimentally produced mouse chimaerae

Development ◽  
1964 ◽  
Vol 12 (4) ◽  
pp. 575-585
Author(s):  
Andrzej K. Tarkowski
Keyword(s):  
Tissue Culture ◽  
Annual Meeting ◽  
Histological Analysis ◽  
Present Report ◽  
General Description ◽  
Tissue Culture Association ◽  
Culture In Vitro ◽  
Pigment Synthesis

A General description of the development of mouse chimaerae and an account of the techniques for their production were given in previous reports (Tarkowski, 1961, 1963). The chimaeric character of the embryos and young obtained was tentatively claimed in the first of these publications because (1) the actual union of two eggs into one blastocyst was seen in culture in vitro, (2) of the occurrence of intersexes, (3) pigment synthesis of the types of the dark component occurred in the majority of individuals developed from pairs of eggs differing genetically in factors for pigmentation. The last criterion was met only by macroscopic search for pigment in the eyes. The present report gives a more detailed description of the distribution of pigment forming cells in these animals, based on histological analysis. Some remarks on the validity and applicability of such a criterion for estimating the degree of chimaerism were made at the 13th Annual Meeting of the Tissue Culture Association (Tarkowski 1963).

scholarly journals In vitro callus induction and plantlet regeneration of Indian red wood, Soymida febrifuga A. Juss (Roxb.)

2014 ◽  
Vol 8 (24) ◽  
pp. 847-856
Author(s):  
K. Chiruvella Kishore ◽  
Mohammed Arifullah ◽  
Gopal Ghanta Rama ◽  
K. Chiruvella Kishore ◽  
Mohammed Arifullah ◽  
...  
Keyword(s):  
Callus Induction ◽  
Plantlet Regeneration

In vitro plantlet regeneration of Olea europaea ssp. maderensis

2003 ◽  
Vol 97 (1) ◽  
pp. 83-87 ◽  
Author(s):  
Conceição V. Santos ◽  
Gina Brito ◽  
Gloria Pinto ◽  
Henrique M.A.C. Fonseca
Keyword(s):  
Olea Europaea ◽  
Plantlet Regeneration ◽  
Olea Europaéa

scholarly journals In vitro regeneration performance of Corchorus olitorius

1970 ◽  
Vol 8 (1) ◽  
pp. 1-6 ◽  
Author(s):  
M Hoque ◽  
KM Nasiruddin ◽  
GKMN Haque ◽  
GC Biswas
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
Root Formation ◽  
Plantlet Regeneration ◽  
Ms Medium ◽  
Corchorus Olitorius ◽  
The Media ◽  
Regenerated Plantlets

The experiment was conducted during May to December 2008 in the Biotechnology Laboratory of Bangladesh Agricultural University, Mymensingh to observe the callus induction, regeneration potentiality and to establish a suitable in vitro plantlet regeneration protocol of Corchorus olitorius. MS medium supplemented with different phytohormone concentrations and combinations were used to observe the callus induction, shoot regeneration and root formation ability of the cotyledon with attached petiole derived explant of three genotypes viz. O-9897, O-72 and OM-1. The highest callus induction (92.85%) was observed in O-9897 followed by O-72 (82.14%) in the MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA. Genotype O-9897 in MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA produced the highest percentage of shoot regenerants (83.33%) followed by O-72 (75.00%) in the media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA. The root formation from regenerants was the best on halfstrength of MS media supplemented with 0.6 mg/L IBA in genotype O-9897 (45.00%). The in vitro regenerated plantlets from the genotypes O-9897 could be established in the field. Therefore, the genotypes O-9897 of C. olitorius in MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA could be used for callus induction and shoot regeneration. Keywords: Regeneration; Phytohormone; Corchorus olitorius DOI: 10.3329/jbau.v8i1.6390J. Bangladesh Agril. Univ. 8(1): 1-6, 2010

scholarly journals Micropropagation of Mini Orchid Hybrid Phalaenopsis “Sogo Vivien”

2016 ◽  
Vol 1 (1) ◽  
pp. 45 ◽  
Author(s):  
Exsyupransia Mursyanti ◽  
Aziz Purwantoro ◽  
Sukarti Moeljopawiro ◽  
Endang Semiarti
Keyword(s):  
Cell Shape ◽  
Histological Analysis ◽  
The Other ◽  
Mass Propagation ◽  
Flower Stalk ◽  
Shoot Segment ◽  
Plant Body ◽  
Pot Plant ◽  
Variegated Plant

Phalaenopsis “Sogo Vivien” is an orchid hybrid with mini size plant body, and exhibits numerous beautiful pink flowers, that is ideal as ornamental pot plant. Some plants of this orchid exhibit variegated leaves that improve the beauty of the plant, not only because of the flower but also as attracted leaves. This orchid has high economical value, but mass propagation of this orchid has not established yet. An effective method to propagate both the normal and variegated plants is worth to be generated. The objective of this research was to produce a large number of P. “Sogo Vivien” plants, including the variegated plants. The method used seeds from self pollinating variegated plant, and flower stalk nodes. The seeds were sown on three various medium: VW, NP and MS, and flower stalk nodes were planted on VW + BA 10 mg l-1 + active carbon. The results showed that the best medium for in vitro culture of P. “Sogo Vivien” was NP medium, in which all seeds could grew into plantlets. Most plantlets emerged from the seeds were non variegated, only one plantlet out of 1344 seeds was variegated (0.007%). Although all emerged plantlets from flower stalk exhibited variegated leaves. Particularly, the plantlets arised from the second and third basal nodes of flower stalk showed the highest growth rate than that from the other nodes. Histological analysis showed that at 11-13 days after shoot segment plantation on NP medium, the shape of apical cells in the nodes was changed, then followed by the change of cell shape in the basal part of the nodes, produced bipolar pattern, then gradually developed into shoot. These results suggest that mass propagation could be achieved using seed culture, but to get the variegated phenotypes, the second and third nodes of flower stalk from variegated plant were the best explants to be used.

Development of an efficient in vitro regeneration protocol in fig (Ficus carica L.)

2015 ◽  
Vol 17 (02) ◽  
pp. 160-164 ◽  
Author(s):  
S.S. Dhage ◽  
V.P. Chimote ◽  
B.D. Pawar ◽  
A.A. Kale ◽  
S.V. Pawar ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Ficus Carica

scholarly journals Safety of Aqueous Extract of Calea ternifolia Used in Mexican Traditional Medicine

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Ma G. E. González-Yáñez ◽  
Catalina Rivas-Morales ◽  
María A. Oranday-Cárdenas ◽  
María J. Verde-Star ◽  
María A. Núñez-González ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Aqueous Extract ◽  
Histological Analysis ◽  
Liver Enzymes ◽  
Renal Tissue ◽  
Proximal Tubules ◽  
Hepatic Toxicity ◽  
Treatment Of Diabetes

There is a trend to use medicinal plants for primary medical care or as dietary supplements; however, the safety of many of these plants has not been studied. The objective of this work was to determine the toxic effect of the aqueous extract of Calea ternifolia (C. zacatechichi), known popularly as “dream herb” in vivo and in vitro in order to validate its safety. In vivo, the extract had moderate toxicity on A. salina. In vitro, the extract induced eryptosis of 73% at a concentration of 100 μg·mL−1 and it inhibited CYP3A by 99% at a concentration of 375 μg/mL. After administering 8.5 mg/kg of C. ternifolia to rats, we found a reduction in platelets and leukocytes and an increase in urea and the liver enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP). Histological analysis showed spongiform changes in the proximal tubules of renal tissue and a lymphoid infiltrate in liver tissue. This plant is used in the treatment of diabetes, and it is commercialized as a dietary supplement in several countries. Our results show renal and hepatic toxicity; therefore, more profound research on the toxicity of this plant is needed.

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