Aqueous two-phase purification of α-Amylase from white pitaya ( Hylocereus undatus ) peel in polyethylene glycol /citrate system: Optimization by response surface methodology

2018 ◽  
Vol 14 ◽  
pp. 305-313 ◽  
Author(s):  
Zahra Shad ◽  
Hamed Mirhosseini ◽  
Anis Shobirin Meor Hussin ◽  
Bita Forghani ◽  
Mahsa Motshakeri ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Polyethylene Glycol ◽  
Response Surface ◽  
System Optimization ◽  
Two Phase ◽  
Hylocereus Undatus

scholarly journals Response Surface Methodology for the Evaluation of Magnetite Partitioning Behavior in Polyethylene Glycol-Based Aqueous Biphasic System

2019 ◽  
Vol 43 (6) ◽  
pp. 2807-2813 ◽  
Author(s):  
Behnia Shahrokhi ◽  
Mohsen Pirdashti ◽  
Mozhgan Managhebi
Keyword(s):  
Molecular Weight ◽  
Response Surface Methodology ◽  
Polyethylene Glycol ◽  
Response Surface ◽  
Polynomial Equation ◽  
Weight Percent ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Biphasic ◽  
Partitioning Behavior

Abstract Response surface methodology in the framework of central composite design has successfully applied to polyethylene glycol (PEG)-based aqueous two-phase system in order to evaluate its efficiency in extracting Fe from iron ores with respect to PEG molecular weight, PEG concentration and salt concentration factors. Furthermore, ANOVA and multiple regression analysis have provided a second-order polynomial equation that is highly capable of predicting the experimental results. The PEG molecular weight and weight percent of the salt in the feed had a major and minor effects on the iron ion partition coefficient, respectively.

Optimization of Aqueous Two Phase Extraction of Proteins from Litopenaeus Vannamei Waste by Response Surface Methodology Coupled Multi-Objective Genetic Algorithm

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
P. Saravana Pandian ◽  
S. Sindhanai Selvan ◽  
A. Subathira ◽  
S. Saravanan
Keyword(s):  
Genetic Algorithm ◽  
Molecular Weight ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Litopenaeus Vannamei ◽  
Value Added ◽  
Phase System ◽  
Two Phase ◽  
Multi Objective ◽  
Multi Objective Genetic Algorithm

Abstract Waste generated from industrial processing of seafood is an enormous source of commercially valuable proteins. One among the underutilized seafood waste is shrimp waste, which primarily consists of head and carapace. Litopenaeus vannamei (L. vannamei) is the widely cultivated shrimp in Asia and contributes to 90 % of aggregate shrimp production in the world. This work was focused on extraction as well as purification of value-added proteins from L. vannamei waste in a single step aqueous two phase system (ATPS). Polyethylene glycol (PEG) and trisodium citrate system were chosen for the ATPS owing to their adequate partitioning and less toxic nature. Response surface methodology (RSM) was implemented for the optimization of independent process variables such as PEG molecular weight (2000 to 6000), pH (6 to 8) and temperature (25 to 45 °C). The results obtained from RSM were further validated using a Multi-objective genetic algorithm (MGA). At the optimized condition of PEG molecular weight 2000, pH 8 and temperature 35 °C, maximum partition coefficient and protein yield were found to be 2.79 and 92.37 %, respectively. Thus, L. vannamei waste was proved to be rich in proteins, which could be processed industrially through cost-effective non-polluting ATPS extraction, and RSM coupled MGA could be a potential tool for such process optimization.

scholarly journals Application of electrochemical peroxidation (ECP) process for waste-activated sludge stabilization and system optimization using response surface methodology (RSM)

2018 ◽  
Vol 77 (6) ◽  
pp. 1765-1776 ◽  
Author(s):  
Gagik Badalians Gholikandi ◽  
Khashayar Kazemirad
Keyword(s):  
Response Surface Methodology ◽  
Activated Sludge ◽  
Response Surface ◽  
Removal Efficiency ◽  
Ph Value ◽  
System Optimization ◽  
Waste Activated Sludge ◽  
Operational Conditions ◽  
Sludge Stabilization ◽  
Rsm Optimization

Abstract In this study, the performance of the electrochemical peroxidation (ECP) process for removing the volatile suspended solids (VSS) content of waste-activated sludge was evaluated. The Fe2+ ions required by the process were obtained directly from iron electrodes in the system. The performance of the ECP process was investigated in various operational conditions employing a laboratory-scale pilot setup and optimized by response surface methodology (RSM). According to the results, the ECP process showed its best performance when the pH value, current density, H2O2 concentration and the retention time were 3, 3.2 mA/cm2, 1,535 mg/L and 240 min, respectively. In these conditions, the introduced Fe2+ concentration was approximately 500 (mg/L) and the VSS removal efficiency about 74%. Moreover, the results of the microbial characteristics of the raw and the stabilized sludge demonstrated that the ECP process is able to remove close to 99.9% of the coliforms in the raw sludge during the stabilization process. The energy consumption evaluation showed that the required energy of the ECP reactor (about 1.8–2.5 kWh (kg VSS removed)−1) is considerably lower than for aerobic digestion, the conventional waste-activated sludge stabilization method (about 2–3 kWh (kg VSS removed)−1). The RSM optimization process showed that the best operational conditions of the ECP process comply with the experimental results, and the actual and the predicted results are in good conformity with each other. This feature makes it possible to predict the introduced Fe2+ concentrations into the system and the VSS removal efficiency of the process precisely.

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