Structures of hydroxy fatty acids as the constituents of triacylglycerols in Philippine wild edible mushroom, Ganoderma lucidum

2017 ◽  
Vol 12 ◽  
pp. 148-151 ◽  
Author(s):  
Jiann-Tsyh Lin ◽  
Ching T. Hou ◽  
Rich Milton R. Dulay ◽  
Karen Ray ◽  
Grace Q. Chen
Keyword(s):  
Fatty Acids ◽  
Ganoderma Lucidum ◽  
Edible Mushroom ◽  
Hydroxy Fatty Acids ◽  
Wild Edible Mushroom

scholarly journals Fatty Acid Compositions of Six Wild Edible Mushroom Species

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Pelin Günç Ergönül ◽  
Ilgaz Akata ◽  
Fatih Kalyoncu ◽  
Bülent Ergönül
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Edible Mushroom ◽  
Mushroom Species ◽  
Dominant Fatty Acid ◽  
Wild Edible Mushroom ◽  
Fatty Acid Compositions

The fatty acids of six wild edible mushroom species (Boletus reticulatus,Flammulina velutipesvar.velutipes,Lactarius salmonicolor,Pleurotus ostreatus,Polyporus squamosus,andRussula anthracina) collected from different regions from Anatolia were determined. The fatty acids were identified and quantified by gas chromatography and studied using fruit bodies. Fatty acid composition varied among species. The dominant fatty acid in fruit bodies of all mushrooms wascis-linoleic acid (18 : 2). Percentage ofcis-linoleic acid in species varied from 22.39% to 65.29%. The other major fatty acids were, respectively,cis-oleic, palmitic, and stearic acids. Fatty acids analysis of the mushrooms showed that the unsaturated fatty acids were at higher concentrations than saturated fatty acids.

scholarly journals Occurrence of 2-hydroxy fatty acids in animal tissues

1963 ◽  
Vol 4 (2) ◽  
pp. 139-143 ◽  
Author(s):  
Yasuo Kishimoto ◽  
Norman S. Radin
Keyword(s):  
Fatty Acids ◽  
Hydroxy Fatty Acids ◽  
Animal Tissues

scholarly journals Optimization and Engineering of a Self-Sufficient CYP102 Enzyme from Bacillus amyloliquefaciens towards Synthesis of In-Chain Hydroxy Fatty Acids

Catalysts ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 665
Author(s):  
Li Zong ◽  
Yan Zhang ◽  
Zhengkang Shao ◽  
Yingwu Wang ◽  
Zheng Guo ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Palmitic Acid ◽  
Bacillus Amyloliquefaciens ◽  
Catalytic Domain ◽  
Hydroxy Fatty Acids ◽  
Saturation Mutagenesis ◽  
Long Chain Fatty Acids ◽  
Medical Field ◽  
Biotransformation Process ◽  
Total Product

Cytochrome P450 (CYP) mediated enzymatic hydroxylation of fatty acids present a green alternative to chemical synthesis of hydroxy fatty acids (HFAs), which are high-value oleochemicals with various uses in materials industry and medical field. Although many CYPs require the presence of additional reductase proteins for catalytic activity, self-sufficient CYPs have their reductase partner naturally fused into their catalytic domain, leading to a greatly simplified biotransformation process. A recently discovered self-sufficient CYP, BAMF2522 from Bacillus amyloliquefaciens DSM 7, exhibits novel regioselectivity by hydroxylating in-chain positions of palmitic acid generating ω-1 to ω-7 HFAs, a rare regiodiversity profile among CYPs. Besides, F89I mutant of BAMF2522 expanded hydroxylation up to ω-9 position of palmitic acid. Here, we further characterize this enzyme by determining optimum temperature and pH as well as thermal stability. Moreover, using extensive site-directed and site-saturation mutagenesis, we obtained BAMF2522 variants that demonstrate greatly increased regioselectivity for in-chain positions (ω-4 to ω-9) of various medium to long chain fatty acids. Remarkably, when a six-residue mutant was reacted with palmitic acid, 84% of total product content was the sum of ω-7, ω-8 and ω-9 HFA products, the highest in-chain selectivity observed to date with a self-sufficient CYP. In short, our study demonstrates the potential of a recently identified CYP and its mutants for green and sustainable production of a variety of in-chain hydroxy enriched HFAs.

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