Thermal stability of Candida antarctica lipase B immobilized on macroporous acrylic resin particles in organic media

2013 ◽  
Vol 2 (1) ◽  
pp. 7-11 ◽  
Author(s):  
Yadagiri Poojari ◽  
Stephen J. Clarson
Keyword(s):  
Thermal Stability ◽  
Acrylic Resin ◽  
Candida Antarctica ◽  
Candida Antarctica Lipase B ◽  
Organic Media ◽  
Candida Antarctica Lipase ◽  
Lipase B ◽  
Thermal Stability Of

Immobilization of Candida antarctica lipase B onto Purolite® MN102 and its application in solvent-free and organic media esterification

2016 ◽  
Vol 40 (1) ◽  
pp. 23-34 ◽  
Author(s):  
Marija Ćorović ◽  
Mladen Mihailović ◽  
Katarina Banjanac ◽  
Milica Carević ◽  
Ana Milivojević ◽  
...  
Keyword(s):  
Candida Antarctica ◽  
Solvent Free ◽  
Candida Antarctica Lipase B ◽  
Organic Media ◽  
Candida Antarctica Lipase ◽  
Lipase B

Over-Stabilization of Chemically Modified and Cross-Linked Candida antarctica Lipase B Using Various Epoxides and Diepoxides

2009 ◽  
Vol 62 (8) ◽  
pp. 799 ◽  
Author(s):  
Nemanja Miletić ◽  
Katja Loos
Keyword(s):  
Thermal Stability ◽  
Kinetic Resolution ◽  
Structural Rearrangement ◽  
Candida Antarctica ◽  
Candida Antarctica Lipase B ◽  
Cross Linking ◽  
Candida Antarctica Lipase ◽  
Lipase B ◽  
Chemically Modified ◽  
Spacer Arm

Candida antarctica lipase B, Cal-B, was (i) chemically modified with various epoxides, or (ii) cross-linked with various diepoxides in order to improve enzyme activity and thermal stability. Changed enzyme structure was confirmed by kinetic resolution of p-nitrophenol acetate with methanol and determination of the kinetic parameters. Thermal stability measurements were carried out for each Cal-B derivative, proving that Cal-B modified with 1,2-epoxypropane and 1,2-epoxypentane, and cross-linked with 1,2,7,8-diepoxyoctane and 1,2,9,10-diepoxydecane showed higher stability than soluble enzyme. The influence of the length of the epoxide arm in the modification process, as well as the length of the spacer arm in the cross-linking process, on the final enzyme preparation properties was investigated. The amount of epoxides and diepoxides used for structural rearrangement played an important role in the derivatives’ characteristics. The influence of the precipitant used and the presence of additives, such as surfactant or crown-ether, during modification and cross-linking processes was also revealed.

Pore-expanded SBA-15 for the immobilization of a recombinant Candida antarctica lipase B: Application in esterification and hydrolysis as model reactions

2018 ◽  
Vol 129 ◽  
pp. 12-24 ◽  
Author(s):  
Nathalia Saraiva Rios ◽  
Maisa Pessoa Pinheiro ◽  
Magno Luís Bezerra Lima ◽  
Denise Maria Guimarães Freire ◽  
Ivanildo José da Silva ◽  
...  
Keyword(s):  
Candida Antarctica ◽  
Candida Antarctica Lipase B ◽  
Candida Antarctica Lipase ◽  
Lipase B ◽  
Model Reactions
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