A combination of small molecules directly reprograms mouse fibroblasts into neural stem cells

2016 ◽  
Vol 476 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Jie Zheng ◽  
Kyung-Ah Choi ◽  
Phil Jun Kang ◽  
Solji Hyeon ◽  
Suhyun Kwon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Small Molecules ◽  
Mouse Fibroblasts

scholarly journals Direct Reprogramming of Mouse Fibroblasts to Neural Stem Cells by Small Molecules

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Yan-Chuang Han ◽  
Yoon Lim ◽  
Michael D. Duffieldl ◽  
Hua Li ◽  
Jia Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Neural Stem Cells ◽  
Small Molecules ◽  
Clinical Medicine ◽  
Viral Vector ◽  
Expression Patterns ◽  
Tumor Formation ◽  
Patient Specific ◽  
Mouse Fibroblasts

Although it is possible to generate neural stem cells (NSC) from somatic cells by reprogramming technologies with transcription factors, clinical utilization of patient-specific NSC for the treatment of human diseases remains elusive. The risk hurdles are associated with viral transduction vectors induced mutagenesis, tumor formation from undifferentiated stem cells, and transcription factors-induced genomic instability. Here we describe a viral vector-free and more efficient method to induce mouse fibroblasts into NSC using small molecules. The small molecule-induced neural stem (SMINS) cells closely resemble NSC in morphology, gene expression patterns, self-renewal, excitability, and multipotency. Furthermore, the SMINS cells are able to differentiate into astrocytes, functional neurons, and oligodendrocytesin vitroandin vivo. Thus, we have established a novel way to efficiently induce neural stem cells (iNSC) from fibroblasts using only small molecules without altering the genome. Such chemical induction removes the risks associated with current techniques such as the use of viral vectors or the induction of oncogenic factors. This technique may, therefore, enable NSC to be utilized in various applications within clinical medicine.

scholarly journals Generation of Integration-free Induced Neural Stem Cells from Mouse Fibroblasts

2016 ◽  
Vol 291 (27) ◽  
pp. 14199-14212 ◽  
Author(s):  
Sung Min Kim ◽  
Jong-Wan Kim ◽  
Tae Hwan Kwak ◽  
Sang Woong Park ◽  
Kee-Pyo Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Mouse Fibroblasts ◽  
Induced Neural Stem Cells

Direct conversion of mouse fibroblasts into induced neural stem cells

2014 ◽  
Vol 9 (4) ◽  
pp. 871-881 ◽  
Author(s):  
Sung Min Kim ◽  
Hannah Flaßkamp ◽  
Andreas Hermann ◽  
Marcos Jesús Araúzo-Bravo ◽  
Seung Chan Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Direct Conversion ◽  
Mouse Fibroblasts ◽  
Induced Neural Stem Cells

scholarly journals Neural Stem Cells Derived by Small Molecules Preserve Vision

2013 ◽  
Vol 2 (1) ◽  
pp. 1 ◽  
Author(s):  
Bin Lu ◽  
Catherine W. Morgans ◽  
Sergey Girman ◽  
Jing Luo ◽  
Jiagang Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Small Molecules

scholarly journals Small molecules enable OCT4-mediated direct reprogramming into expandable human neural stem cells

Cell Research ◽  
2013 ◽  
Vol 24 (1) ◽  
pp. 126-129 ◽  
Author(s):  
Saiyong Zhu ◽  
Rajesh Ambasudhan ◽  
Woong Sun ◽  
Hyun Jung Kim ◽  
Maria Talantova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Small Molecules ◽  
Direct Reprogramming ◽  
Human Neural Stem Cells

Discovery of small molecules that enhance astrocyte differentiation in rat fetal neural stem cells

2011 ◽  
Vol 21 (23) ◽  
pp. 7050-7053 ◽  
Author(s):  
Dong Jo Chang ◽  
Mi Young Jeong ◽  
Jiho Song ◽  
Chang Yun Jin ◽  
Young-Ger Suh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Small Molecules ◽  
Astrocyte Differentiation
Sign in / Sign up

Export Citation Format

Share Document