Development of a monoclonal antibody specific to envelope domain III with broad-spectrum detection of all four dengue virus serotypes

2016 ◽  
Vol 473 (4) ◽  
pp. 894-898 ◽  
Author(s):  
Sae-Hae Kim ◽  
Yu Na Kim ◽  
Thang Thua Truong ◽  
Nguyen Thi Thu Thuy ◽  
Le Quynh Mai ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Dengue Virus ◽  
Broad Spectrum ◽  
Spectrum Detection ◽  
Dengue Virus Serotypes ◽  
Domain Iii

scholarly journals A broadly neutralizing germline-like human monoclonal antibody against dengue virus envelope domain III

2019 ◽  
Vol 15 (6) ◽  
pp. e1007836 ◽  
Author(s):  
Dan Hu ◽  
Zhongyu Zhu ◽  
Shun Li ◽  
Yongqiang Deng ◽  
Yanling Wu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Dengue Virus ◽  
Human Monoclonal Antibody ◽  
Virus Envelope ◽  
Domain Iii

Generation and characterization of a monoclonal antibody that cross-reacts with the envelope protein from the four dengue virus serotypes

Apmis ◽  
2013 ◽  
Vol 121 (9) ◽  
pp. 848-858 ◽  
Author(s):  
Moisés León-Juárez ◽  
Julio García-Cordero ◽  
Leopoldo Santos-Argumedo ◽  
Héctor Romero-Ramírez ◽  
Jazmín García-Machorro ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Dengue Virus ◽  
Envelope Protein ◽  
Dengue Virus Serotypes

scholarly journals Phage display demonstrates durable differences in serological profile by route of inoculation in primary infections of non-human primates with Dengue Virus 1

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jayant V. Rajan ◽  
Michael McCracken ◽  
Caleigh Mandel-Brehm ◽  
Greg Gromowski ◽  
Simon Pollett ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Phage Display ◽  
Denv Infection ◽  
Phage Library ◽  
Humoral Immune ◽  
Linear Peptide ◽  
Humoral Immune Responses ◽  
High Resolution Map ◽  
Domain Iii ◽  
Inoculation Route

AbstractNatural dengue virus (DENV) infections occur by mosquito bite but how the inoculation route affects the humoral immune response is unknown. We serologically profiled 20 non-human primates (NHP) from a prior study of DENV1 infection where animals were inoculated by mosquito (N = 10) or subcutaneous injection (N = 10). Using a comprehensive, densely tiled and highly redundant pan-flavivirus programmable phage library containing 91,562 overlapping 62 amino acid peptides, we produced a high-resolution map of linear peptide sequences enriched during DENV seroconversion. Profiles in mosquito-inoculated and subcutaneously-inoculated animals were similar up to 90 days after primary infection, but diverged at 1 year with differences in sero-reactivity in the Envelope (E; residues 215–406; p < 0.08), and Nonstructural-3 (NS3; residues 549–615; p < 0.05) proteins in mosquito-inoculated versus subcutaneously-inoculated animals. Within the E protein, residues 339–384 in domain III accounted for > 99% of the observed sero-reactivity difference. Antibody breadth did not vary by mode of inoculation. The differential reactivity to E domain III seen by phage display validated orthogonally by ELISA, but did not correlate with late neutralization titers. Serological profiling of humoral immune responses to DENV infection in NHP by programmable phage display demonstrated durable differences in sero-reactivity by route of inoculation.

scholarly journals ACE2-targeting monoclonal antibody as potent and broad-spectrum coronavirus blocker

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Yuning Chen ◽  
Ya-Nan Zhang ◽  
Renhong Yan ◽  
Guifeng Wang ◽  
Yuanyuan Zhang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Broad Spectrum ◽  
Management Strategy ◽  
Spectrum Management ◽  
Clinical Development ◽  
Severe Toxicity ◽  
Converting Enzyme ◽  
Angiotensin Converting Enzyme 2 ◽  
Binding Residues

AbstractThe evolution of coronaviruses, such as SARS-CoV-2, makes broad-spectrum coronavirus preventional or therapeutical strategies highly sought after. Here we report a human angiotensin-converting enzyme 2 (ACE2)-targeting monoclonal antibody, 3E8, blocked the S1-subunits and pseudo-typed virus constructs from multiple coronaviruses including SARS-CoV-2, SARS-CoV-2 mutant variants (SARS-CoV-2-D614G, B.1.1.7, B.1.351, B.1.617.1, and P.1), SARS-CoV and HCoV-NL63, without markedly affecting the physiological activities of ACE2 or causing severe toxicity in ACE2 “knock-in” mice. 3E8 also blocked live SARS-CoV-2 infection in vitro and in a prophylactic mouse model of COVID-19. Cryo-EM and “alanine walk” studies revealed the key binding residues on ACE2 interacting with the CDR3 domain of 3E8 heavy chain. Although full evaluation of safety in non-human primates is necessary before clinical development of 3E8, we provided a potentially potent and “broad-spectrum” management strategy against all coronaviruses that utilize ACE2 as entry receptors and disclosed an anti-coronavirus epitope on human ACE2.

Colloidal gold immunochromatographic test strips for broad-spectrum detection of Salmonella

Food Control ◽  
2021 ◽  
pp. 108052
Author(s):  
Youxue Wu ◽  
Meijiao Wu ◽  
Cheng Liu ◽  
Yachen Tian ◽  
Shuiqin Fang ◽  
...  
Keyword(s):  
Broad Spectrum ◽  
Colloidal Gold ◽  
Immunochromatographic Test ◽  
Test Strips ◽  
Spectrum Detection
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