scholarly journals Stimulation of the Streptococcus pneumoniae RecA protein-promoted three-strand exchange reaction by the competence-specific SsbB protein

2012 ◽  
Vol 424 (1) ◽  
pp. 40-44 ◽  
Author(s):  
Diane E. Grove ◽  
Geetha Anne ◽  
Mohammad A. Hedayati ◽  
Floyd R. Bryant
Keyword(s):  
Streptococcus Pneumoniae ◽  
Exchange Reaction ◽  
Reca Protein ◽  
Strand Exchange ◽  
Stimulation Of

scholarly journals Effect of theStreptococcus pneumoniaeMmsA Protein on the RecA Protein-promoted Three-strand Exchange Reaction

2002 ◽  
Vol 277 (28) ◽  
pp. 24863-24869 ◽  
Author(s):  
Mohammad A. Hedayati ◽  
Scott E. Steffen ◽  
Floyd R. Bryant
Keyword(s):  
Exchange Reaction ◽  
Reca Protein ◽  
Strand Exchange

Appropriate initiation of the strand exchange reaction promoted by RecA protein requires ATP hydrolysis

2001 ◽  
Vol 309 (1) ◽  
pp. 29-43 ◽  
Author(s):  
Zhaoqing Zhang ◽  
Dennis Yoon ◽  
Jacob R LaPorte ◽  
Junghuei Chen
Keyword(s):  
Exchange Reaction ◽  
Atp Hydrolysis ◽  
Reca Protein ◽  
Strand Exchange

scholarly journals A novel pairing process promoted by Escherichia coli RecA protein: inverse DNA and RNA strand exchange

2000 ◽  
Vol 14 (6) ◽  
pp. 740-749
Author(s):  
Eugene N. Zaitsev ◽  
Stephen C. Kowalczykowski
Keyword(s):  
Escherichia Coli ◽  
Exchange Reaction ◽  
Reca Protein ◽  
Strand Exchange ◽  
Loop Formation ◽  
Dna And Rna ◽  
Double Stranded Dna ◽  
Dna Strand Exchange ◽  
Dna Strand ◽  
Recombination Reactions

Traditionally, recombination reactions promoted by RecA-like proteins initiate by forming a nucleoprotein filament on a single-stranded DNA (ssDNA), which then pairs with homologous double-stranded DNA (dsDNA). In this paper, we describe a novel pairing process that occurs in an unconventional manner: RecA protein polymerizes along dsDNA to form an active nucleoprotein filament that can pair and exchange strands with homologous ssDNA. Our results demonstrate that this “inverse” reaction is a unique, highly efficient DNA strand exchange reaction that is not due to redistribution of RecA protein from dsDNA to the homologous ssDNA partner. Finally, we demonstrate that the RecA protein–dsDNA filament can also pair and promote strand exchange with ssRNA. This inverse RNA strand exchange reaction is likely responsible for R-loop formation that is required for recombination-dependent DNA replication.

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