The characteristics of genome-wide DNA methylation in naïve CD4+ T cells of patients with psoriasis or atopic dermatitis

2012 ◽  
Vol 422 (1) ◽  
pp. 157-163 ◽  
Author(s):  
Jihye Han ◽  
Sin-Gi Park ◽  
Jae-Bum Bae ◽  
JungKyoon Choi ◽  
Jae-Myun Lyu ◽  
...  
Keyword(s):  
Dna Methylation ◽  
T Cells ◽  
Atopic Dermatitis ◽  
Cd4 T Cells ◽  
Genome Wide

scholarly journals Genome wide DNA methylation landscape reveals glioblastoma’s influence on epigenetic changes in tumor infiltrating CD4+ T cells

Oncotarget ◽  
2021 ◽  
Author(s):  
Marpe Bam ◽  
Sreenivasulu Chintala ◽  
Kaleigh Fetcko ◽  
Brooke Carmen Williamsen ◽  
Seema Siraj ◽  
...  
Keyword(s):  
Dna Methylation ◽  
T Cells ◽  
Cd4 T Cells ◽  
Epigenetic Changes ◽  
Genome Wide

Methylation differences at the HLA-DRB1 locus in CD4+ T-Cells are associated with multiple sclerosis

2013 ◽  
Vol 20 (8) ◽  
pp. 1033-1041 ◽  
Author(s):  
MC Graves ◽  
M Benton ◽  
RA Lea ◽  
M Boyle ◽  
L Tajouri ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Dna Methylation ◽  
T Cells ◽  
Cd4 T Cells ◽  
Cpg Island ◽  
Major Effect ◽  
Differential Methylation ◽  
Genome Wide ◽  
A Genome ◽  
Drb1 Locus

Background: Multiple sclerosis (MS) is thought to be caused by T-cell mediated autoimmune dysfunction. Risk of developing MS is influenced by environmental and genetic factors. Modifiable differences in DNA methylation are recognized as epigenetic contributors to MS risk and may provide a valuable link between environmental exposure and inherited genetic systems. Objectives and methods: To identify methylation changes associated with MS, we performed a genome-wide DNA methylation analysis of CD4+ T cells from 30 patients with relapsing–remitting MS and 28 healthy controls using Illumina 450K methylation arrays. Results: A striking differential methylation signal was observed at chr. 6p21, with a peak signal at HLA-DRB1. After prioritisation, we identified a panel of 74 CpGs associated with MS in this cohort. Most notably we found evidence of a major effect CpG island in DRB1 in MS cases ( pFDR < 3 × 10−3). In addition, we found 55 non-HLA CpGs that exhibited differential methylation, many of which localise to genes previously linked to MS. Conclusions: Our findings provide the first evidence for association of DNA methylation at HLA-DRB1 in relation to MS risk. Further studies are now warranted to validate and understand how these findings are involved in MS pathology.

Genome-wide DNA methylation patterns in CD4+ T cells from Chinese Han patients with rheumatoid arthritis

2016 ◽  
Vol 27 (3) ◽  
pp. 441-447 ◽  
Author(s):  
Shicheng Guo ◽  
Qi Zhu ◽  
Ting Jiang ◽  
Rongsheng Wang ◽  
Yi Shen ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Dna Methylation ◽  
T Cells ◽  
Cd4 T Cells ◽  
Chinese Han ◽  
Genome Wide ◽  
Methylation Patterns

scholarly journals Genome-Wide DNA Methylation Patterns in Naive CD4+ T Cells From Patients With Primary Sjögren's Syndrome

2014 ◽  
Vol 66 (3) ◽  
pp. 731-739 ◽  
Author(s):  
Nezam Altorok ◽  
Patrick Coit ◽  
Travis Hughes ◽  
Kristi A. Koelsch ◽  
Donald U. Stone ◽  
...  
Keyword(s):  
Dna Methylation ◽  
T Cells ◽  
Cd4 T Cells ◽  
Sjogren’S Syndrome ◽  
Sjogren's Syndrome ◽  
Primary Sjögren’S Syndrome ◽  
Primary Sjogren’S Syndrome ◽  
Primary Sjögren's Syndrome ◽  
Genome Wide ◽  
Methylation Patterns

scholarly journals Genome-wide DNA methylation study suggests epigenetic accessibility and transcriptional poising of interferon-regulated genes in naïve CD4+ T cells from lupus patients

2013 ◽  
Vol 43 ◽  
pp. 78-84 ◽  
Author(s):  
Patrick Coit ◽  
Matlock Jeffries ◽  
Nezam Altorok ◽  
Mikhail G. Dozmorov ◽  
Kristi A. Koelsch ◽  
...  
Keyword(s):  
Dna Methylation ◽  
T Cells ◽  
Cd4 T Cells ◽  
Genome Wide

scholarly journals Genome-wide DNA methylation patterns in CD4+ T cells from patients with systemic lupus erythematosus

Epigenetics ◽  
2011 ◽  
Vol 6 (5) ◽  
pp. 593-601 ◽  
Author(s):  
Matlock Jeffries ◽  
Mikhail Dozmorov ◽  
Yuhong Tang ◽  
Joan T. Merrill ◽  
Jonathan D. Wren ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Dna Methylation ◽  
T Cells ◽  
Lupus Erythematosus ◽  
Cd4 T Cells ◽  
Systemic Lupus ◽  
Genome Wide ◽  
Methylation Patterns

scholarly journals 035 Expansion of bacterial phosphatidylglycerol reactive CD4+ T cells in atopic dermatitis

2021 ◽  
Vol 141 (5) ◽  
pp. S6
Author(s):  
G.C. Monnot ◽  
M. Wegrecki ◽  
B.N. Sallee ◽  
L.A. Bordone ◽  
C.H. Rohde ◽  
...  
Keyword(s):  
T Cells ◽  
Atopic Dermatitis ◽  
Cd4 T Cells

scholarly journals Identification of novel genes involved in apoptosis of HIV-infected macrophages using unbiased genome-wide screening

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Simon X. M. Dong ◽  
Frederick S. Vizeacoumar ◽  
Kalpana K. Bhanumathy ◽  
Nezeka Alli ◽  
Cristina Gonzalez-Lopez ◽  
...  
Keyword(s):  
T Cells ◽  
Hiv Infection ◽  
Respiratory Chain ◽  
Cd4 T Cells ◽  
Chain Complex ◽  
Respiratory Chain Complex ◽  
Complex Ii ◽  
Genome Wide ◽  
Latently Infected ◽  
Induced Apoptosis

Abstract Background Macrophages, besides resting latently infected CD4+ T cells, constitute the predominant stable, major non-T cell HIV reservoirs. Therefore, it is essential to eliminate both latently infected CD4+ T cells and tissue macrophages to completely eradicate HIV in patients. Until now, most of the research focus is directed towards eliminating latently infected CD4+ T cells. However, few approaches have been directed at killing of HIV-infected macrophages either in vitro or in vivo. HIV infection dysregulates the expression of many host genes essential for the survival of infected cells. We postulated that exploiting this alteration may yield novel targets for the selective killing of infected macrophages. Methods We applied a pooled shRNA-based genome-wide approach by employing a lentivirus-based library of shRNAs to screen novel gene targets whose inhibition should selectively induce apoptosis in HIV-infected macrophages. Primary human MDMs were infected with HIV-eGFP and HIV-HSA viruses. Infected MDMs were transfected with siRNAs specific for the promising genes followed by analysis of apoptosis by flow cytometry using labelled Annexin-V in HIV-infected, HIV-exposed but uninfected bystander MDMs and uninfected MDMs. The results were analyzed using student’s t-test from at least four independent experiments. Results We validated 28 top hits in two independent HIV infection models. This culminated in the identification of four target genes, Cox7a2, Znf484, Cstf2t, and Cdk2, whose loss-of-function induced apoptosis preferentially in HIV-infected macrophages. Silencing these single genes killed significantly higher number of HIV-HSA-infected MDMs compared to the HIV-HSA-exposed, uninfected bystander macrophages, indicating the specificity in the killing of HIV-infected macrophages. The mechanism governing Cox7a2-mediated apoptosis of HIV-infected macrophages revealed that targeting respiratory chain complex II and IV genes also selectively induced apoptosis of HIV-infected macrophages possibly through enhanced ROS production. Conclusions We have identified above-mentioned novel genes and specifically the respiratory chain complex II and IV genes whose silencing may cause selective elimination of HIV-infected macrophages and eventually the HIV-macrophage reservoirs. The results highlight the potential of the identified genes as targets for eliminating HIV-infected macrophages in physiological environment as part of an HIV cure strategy.

scholarly journals Trichloroethylene-induced alterations in DNA methylation were enriched in polycomb protein binding sites in effector/memory CD4+ T cells

2017 ◽  
Vol 3 (3) ◽  
Author(s):  
Kathleen M. Gilbert ◽  
Sarah J. Blossom ◽  
Brad Reisfeld ◽  
Stephen W. Erickson ◽  
Kanan Vyas ◽  
...  
Keyword(s):  
Dna Methylation ◽  
T Cells ◽  
Protein Binding ◽  
Binding Sites ◽  
Cd4 T Cells ◽  
Effector Memory ◽  
Protein Binding Sites ◽  
Polycomb Protein ◽  
Memory Cd4 T Cells
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