GABA induction of the Saccharomyces cerevisiae UGA4 gene depends on the quality of the carbon source: Role of the key transcription factors acting in this process

2012 ◽  
Vol 421 (3) ◽  
pp. 572-577 ◽  
Author(s):  
Carolina E. Levi ◽  
Sabrina B. Cardillo ◽  
Santiago Bertotti ◽  
Cristian Ríos ◽  
Susana Correa García ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcription Factors ◽  
Carbon Source

Trans-acting regulatory variation in Saccharomyces cerevisiae and the role of transcription factors

2003 ◽  
Vol 35 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Gaël Yvert ◽  
Rachel B Brem ◽  
Jacqueline Whittle ◽  
Joshua M Akey ◽  
Eric Foss ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcription Factors ◽  
Regulatory Variation

The rRNA enhancer regulates rRNA transcription in Saccharomyces cerevisiae

1993 ◽  
Vol 13 (2) ◽  
pp. 1283-1289
Author(s):  
B E Morrow ◽  
S P Johnson ◽  
J R Warner
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Carbon Source ◽  
Binding Sites ◽  
Major Element ◽  
Transcription Unit ◽  
Rrna Genes ◽  
Nutritional Regulation ◽  
Rrna Transcription ◽  
Enhancer Function

In Saccharomyces cerevisiae, the rRNA genes are organized as a tandem array of head-to-tail repeats. An enhancer of rRNA transcription is present just at the end of each transcription unit, 2 kb away from the next one. This enhancer is unusual for S. cerevisiae in that it acts both upstream and downstream of, and even across, genes. The role of the enhancer in the nutritional regulation of rRNA transcription was studied by introducing a centromere plasmid carrying two rRNA minigenes in tandem, flanking a single enhancer, into cells. Analysis of the transcripts from the two minigenes showed that the enhancer was absolutely required for the stimulation of transcription of rRNA that occurs when cells are shifted from a poor carbon source to a good carbon source. While full enhancer function is provided by a 45-bp region at the 3' end of the 190-bp enhancer, some activity was also conferred by other elements, including both a T-rich stretch and a region containing the binding sites for the proteins Reb1p and Abf1p. We conclude that the enhancer is composed of redundant elements and that it is a major element in the regulation of rRNA transcription.

scholarly journals The rRNA enhancer regulates rRNA transcription in Saccharomyces cerevisiae.

1993 ◽  
Vol 13 (2) ◽  
pp. 1283-1289 ◽  
Author(s):  
B E Morrow ◽  
S P Johnson ◽  
J R Warner
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Carbon Source ◽  
Binding Sites ◽  
Major Element ◽  
Transcription Unit ◽  
Rrna Genes ◽  
Nutritional Regulation ◽  
Rrna Transcription ◽  
Enhancer Function

In Saccharomyces cerevisiae, the rRNA genes are organized as a tandem array of head-to-tail repeats. An enhancer of rRNA transcription is present just at the end of each transcription unit, 2 kb away from the next one. This enhancer is unusual for S. cerevisiae in that it acts both upstream and downstream of, and even across, genes. The role of the enhancer in the nutritional regulation of rRNA transcription was studied by introducing a centromere plasmid carrying two rRNA minigenes in tandem, flanking a single enhancer, into cells. Analysis of the transcripts from the two minigenes showed that the enhancer was absolutely required for the stimulation of transcription of rRNA that occurs when cells are shifted from a poor carbon source to a good carbon source. While full enhancer function is provided by a 45-bp region at the 3' end of the 190-bp enhancer, some activity was also conferred by other elements, including both a T-rich stretch and a region containing the binding sites for the proteins Reb1p and Abf1p. We conclude that the enhancer is composed of redundant elements and that it is a major element in the regulation of rRNA transcription.

scholarly journals Roles of the Transcription Factors Sfl2 and Efg1 in White-Opaque Switching in a/α Strains ofCandida albicans

mSphere ◽  
2019 ◽  
Vol 4 (2) ◽  
Author(s):  
Yang-Nim Park ◽  
Kayla Conway ◽  
Thomas P. Conway ◽  
Karla J. Daniels ◽  
David R. Soll
Keyword(s):  
Candida Albicans ◽  
Transcription Factors ◽  
Carbon Source ◽  
Environmental Conditions ◽  
Fungal Pathogen ◽  
Type Locus ◽  
Content Type ◽  
Physiological Conditions ◽  
Ofcandida Albicans

ABSTRACTCandida albicansremains the most pervasive fungal pathogen colonizing humans. The majority of isolates from hosts are heterozygous at the mating type locus (MTLa/α), and a third of these have recently been shown to be capable of switching to the opaque phenotype. Here we have investigated the roles of two transcription factors (TFs) Sfl2 and Efg1, in repressing switching ina/α strains. Deleting either gene results in the capacity ofa/α cells to switch to opaque en masse under facilitating environmental conditions, which includeN-acetylglucosamine (GlcNAc) as the carbon source, physiological temperature (37°C), and high CO2(5%). These conditions are similar to those in the host. Our results further reveal that while glucose is a repressor ofsfl2Δ andefg1Δ switching, GlcNAc is an inducer. Finally, we show that when GlcNAc is the carbon source, and the temperature is low (25°C), theefg1Δ mutants, but not thesfl2Δ mutants, form a tiny, elongate cell, which differentiates into an opaque cell when transferred to conditions optimal fora/α switching. These results demonstrate that at least two TFs, Sfl2 and Efg1, repress switching ina/α cells and thata/α strains with either ansfl2Δ orefg1Δ mutation can switch en masse but only under physiological conditions. The role of opaquea/α cells in commensalism and pathogenesis must, therefore, be investigated.IMPORTANCEMore than 95% ofCandida albicansstrains isolated from humans areMTLa/α, and approximately a third of these can undergo the white-to-opaque transition. Therefore, besides being a requirement forMTL-homozygous strains to mate, the opaque phenotype very likely plays a role in the commensalism and pathogenesis of nonmating,a/α populations colonizing humans.

scholarly journals Ste12 and Ste12-Like Proteins, Fungal Transcription Factors Regulating Development and Pathogenicity

2010 ◽  
Vol 9 (4) ◽  
pp. 480-485 ◽  
Author(s):  
Joanne Wong Sak Hoi ◽  
Bernard Dumas
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcription Factors ◽  
Filamentous Fungi ◽  
Recent Literature ◽  
Fungal Development ◽  
Content Type ◽  
Pseudohyphal Growth ◽  
Life Styles ◽  
Fungal Kingdom

ABSTRACT Ste12 and Ste12-like proteins are transcription factors found exclusively in the fungal kingdom. In the yeast model Saccharomyces cerevisiae, where the first member was identified, Ste12p was shown to regulate mating and invasive/pseudohyphal growth. In recent literature, there have been several reports of Ste12-like factors in multiple fungal systems, yeasts or filamentous fungi, with saprophytic or parasitic life-styles. In all these models, Ste12 and Ste12-like factors are involved in the regulation of fungal development and pathogenicity. In this review, we discuss the features, the regulation, and the role of Ste12 and Ste12-like factors by highlighting the similarities and dissimilarities that occur within this group.

scholarly journals Key Role of Ser562/661 in Snf1-Dependent Regulation of Cat8p in Saccharomyces cerevisiae and Kluyveromyces lactis

2004 ◽  
Vol 24 (10) ◽  
pp. 4083-4091 ◽  
Author(s):  
Godefroid Charbon ◽  
Karin D. Breunig ◽  
Ruddy Wattiez ◽  
Jean Vandenhaute ◽  
Isabelle Noël-Georis
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Carbon Source ◽  
Target Genes ◽  
Kluyveromyces Lactis ◽  
Active Form ◽  
Phosphorylation Site ◽  
Carbon Sources ◽  
Mutant Form ◽  
Zinc Cluster

ABSTRACT Utilization of nonfermentable carbon sources by Kluyveromyces lactis and Saccharomyces cerevisiae requires the Snf1p kinase and the Cat8p transcriptional activator, which binds to carbon source-responsive elements of target genes. We demonstrate that KlSnf1p and KlCat8p from K. lactis interact in a two-hybrid system and that the interaction is stronger with a kinase-dead mutant form of KlSnf1p. Of two putative phosphorylation sites in the KlCat8p sequence, serine 661 was identified as a key residue governing KlCat8p regulation. Serine 661 is located in the middle homology region, a regulatory domain conserved among zinc cluster transcription factors, and is part of an Snf1p consensus phosphorylation site. Single mutations at this site are sufficient to completely change the carbon source regulation of the KlCat8p transactivation activity observed. A serine-to-glutamate mutant form mimicking constitutive phosphorylation results in a nearly constitutively active form of KlCat8p, while a serine-to-alanine mutation has the reverse effect. Furthermore, it is shown that KlCat8p phosphorylation depends on KlSNF1. The Snf1-Cat8 connection is evolutionarily conserved: mutation of corresponding serine 562 of ScCat8p gave similar results in S. cerevisiae. The enhanced capacity of ScCat8S562E to suppress the phenotype caused by snf1 strengthens the hypothesis of direct phosphorylation of Cat8p by Snf1p. Unlike that of S. cerevisiae ScCAT8, KlCAT8 transcription is not carbon source regulated, illustrating the prominent role of posttranscriptional regulation of Cat8p in K. lactis.

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