Identification of a novel histone H3 specific protease activity in nuclei of chicken liver

2012 ◽  
Vol 421 (2) ◽  
pp. 261-267 ◽  
Author(s):  
Papita Mandal ◽  
Gajendra K. Azad ◽  
Raghuvir S. Tomar
Keyword(s):  
Protease Activity ◽  
Histone H3 ◽  
Chicken Liver ◽  
Specific Protease

Chicken liver glutamate dehydrogenase (GDH) demonstrates a histone H3 specific protease (H3ase) activity in vitro

Biochimie ◽  
2013 ◽  
Vol 95 (11) ◽  
pp. 1999-2009 ◽  
Author(s):  
Jogeswar S. Purohit ◽  
Raghuvir S. Tomar ◽  
Anil K. Panigrahi ◽  
Shashibhal M. Pandey ◽  
Divya Singh ◽  
...  
Keyword(s):  
Glutamate Dehydrogenase ◽  
Histone H3 ◽  
Chicken Liver ◽  
Specific Protease

scholarly journals Identification of bistable populations of Porphyromonas gingivalis that differ in epithelial cell invasion

Microbiology ◽  
2010 ◽  
Vol 156 (10) ◽  
pp. 3052-3064 ◽  
Author(s):  
S. Suwannakul ◽  
G. P. Stafford ◽  
S. A. Whawell ◽  
C. W. I. Douglas
Keyword(s):  
Epithelial Cells ◽  
Porphyromonas Gingivalis ◽  
Cell Invasion ◽  
Protease Activity ◽  
Periodontal Pathogen ◽  
Wild Type ◽  
Oral Epithelial Cells ◽  
Specific Protease ◽  
Oral Epithelial ◽  
First Time

Bistable populations of bacteria give rise to two or more subtypes that exhibit different phenotypes. We have explored whether the periodontal pathogen Porphyromonas gingivalis exhibits bistable invasive phenotypes. Using a modified cell invasion assay, we show for the first time that there are two distinct subtypes within a population of P. gingivalis strains NCTC 11834 and W50 that display differences in their ability to invade oral epithelial cells. The highly invasive subtype invades cells at 10–30-fold higher levels than the poorly invasive subtype and remains highly invasive for approximately 12–16 generations. Analysis of the gingipain activity of these subtypes revealed that the highly invasive type had reduced cell-associated arginine-specific protease activity. The role of Arg-gingipain activity in invasion was verified by enhancement of invasion by rgpAB mutations and by inclusion of an Arg-gingipain inhibitor in invasion assays using wild-type bacteria. In addition, a population of ΔrgpAB bacteria did not contain a hyperinvasive subtype. Screening of the protease activity of wild-type populations of both strains identified high and low protease subtypes which also showed a corresponding reduction or enhancement, respectively, of invasive capabilities. Microarray analysis of these bistable populations revealed a putative signature set of genes that includes oxidative stress resistance and iron transport genes, and which might be critical to invasion of or survival within epithelial cells.

scholarly journals Optimasi Waktu Inkubasi Lactobacillus rhamnosus SKG 34 Dalam Produksi Enzim Penggumpal Susu

2020 ◽  
Vol 9 (2) ◽  
pp. 108
Author(s):  
Wulandari Setiadarma ◽  
Dewa Gede Mayun Permana ◽  
Komang Ayu Nocianitri
Keyword(s):  
Incubation Time ◽  
Protease Activity ◽  
Block Design ◽  
Lactobacillus Rhamnosus ◽  
Duncan Multiple Range Test ◽  
Milk Clotting ◽  
Randomized Block Design ◽  
Milk Clotting Enzyme ◽  
Specific Protease ◽  
Range Test

This study aims to determine the effect of incubation time on milk clotting enzyme (MCE) activity produced by L. rhamnosus SKG 34 and determine the optimum incubation time to produce the highest its activity. This study used a randomized block design (RBD) with incubation time as a treatment consisting of 8 levels, that were 6 hours, 12 hours, 18 hours, 24 hours, 30 hours, 36 hours, 42 hours, and 48 hours. Data were analyzed with Variance Analysis (ANOVA) than followed by Duncan Multiple Range Test (DMRT). The analyzed were repeated 3 times resulting in 24 experimental units. The results showed that the incubation time significantly affected protease activity, MCE activity, specific protease activity and ratio of MCE to protease but did not affect the total LAB. The optimum incubation time of L. rhamnosus SKG 34 is 12 hours with total LAB 1.83 x 109 CFU/ml and protease activity 180.67 U/ml, MCE activity 595.06 SU, protease spesific activity 73.149 U/mg and ratio of MCE to protease 3.29 SU/U. Keywords : incubation time, Enzyme protease, Lactobacillus rhamnosus SKG 34, Milk clotting enzyme.

scholarly journals A Collaborative Screening Program for the Discovery of Inhibitors of HCV NS2/3 cis-Cleaving Protease Activity

2002 ◽  
Vol 7 (2) ◽  
pp. 149-154 ◽  
Author(s):  
Mike Whitney ◽  
Jeffrey H. Stack ◽  
Paul L. Darke ◽  
Wei Zheng ◽  
Joe Terzo ◽  
...  
Keyword(s):  
Small Molecule ◽  
Protease Activity ◽  
High Throughput Screening ◽  
Small Molecule Inhibitors ◽  
Screening Program ◽  
Cis Acting ◽  
Specific Protease ◽  
A Cell ◽  
Infectious Cycle ◽  
Genetic Mutants

This report describes the development of a cell-based assay for high-throughput screening and detection of small-molecule inhibitors for hepatitis C virus (HCV) NS2/3 protease. The HCV NS2/3 protease is essential for the normal infectious cycle of HCV. Generation of a cell-based assay for this cis-acting viral protease involved reporter constructs in which the NS2/3 protease sequence was inserted between the β-lactamase (BLA) reporter and a ubiquitin-based destabilization domain. In stable cell lines, NS2/3 cis cleavage of the NS2/3-BLA fusion protein resulted in differential stability of the cleaved versus uncleaved BLA reporter, providing a robust readout for protease activity. BLA reporter activity was shown to be a function of NS2/3-specific protease activity, by using genetic mutants of the NS2/3 sequence. In addition, the cell-based assay was validated and screened in a 384-well format on a fully automated robotic platform where small-molecule inhibitors of NS2/3 protease activity were identified.

scholarly journals In vitro Histone H3 Cleavage Assay for Yeast and Chicken Liver H3 Protease

BIO-PROTOCOL ◽  
2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Sakshi Chauhan ◽  
Gajendra Azad ◽  
Raghuvir Tomar
Keyword(s):  
Histone H3 ◽  
Chicken Liver ◽  
Cleavage Assay

scholarly journals A vitamin-B2-sensing mechanism that regulates gut protease activity to impact animal’s food behavior and growth

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Bin Qi ◽  
Marina Kniazeva ◽  
Min Han
Keyword(s):  
Protease Activity ◽  
Regulatory Mechanisms ◽  
Protease Gene ◽  
Food Behavior ◽  
Nutrient Deficiencies ◽  
Vitamin B2 ◽  
Response Behavior ◽  
Food Uptake ◽  
Food Response ◽  
Specific Protease

To survive challenging environments, animals acquired the ability to evaluate food quality in the intestine and respond to nutrient deficiencies with changes in food-response behavior, metabolism and development. However, the regulatory mechanisms underlying intestinal sensing of specific nutrients, especially micronutrients such as vitamins, and the connections to downstream physiological responses in animals remain underexplored. We have established a system to analyze the intestinal response to vitamin B2 (VB2) deficiency in Caenorhabditis elegans, and demonstrated that VB2 level critically impacts food uptake and foraging behavior by regulating specific protease gene expression and intestinal protease activity. We show that this impact is mediated by TORC1 signaling through reading the FAD-dependent ATP level. Thus, our study in live animals uncovers a VB2-sensing/response pathway that regulates food-uptake, a mechanism by which a common signaling pathway translates a specific nutrient signal into physiological activities, and the importance of gut microbiota in supplying micronutrients to animals.

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