Characterization of the plant homolog of Nijmegen breakage syndrome 1: Involvement in DNA repair and recombination

2007 ◽  
Vol 353 (2) ◽  
pp. 394-398 ◽  
Author(s):  
Noriyuki Akutsu ◽  
Kenta Iijima ◽  
Takeo Hinata ◽  
Hiroshi Tauchi
Keyword(s):  
Dna Repair ◽  
Nijmegen Breakage Syndrome ◽  
Plant Homolog

Partial characterization of the DNA repair protein complex, containing the ERCC1, ERCC4, ERCC11 and XPF correcting activities

1995 ◽  
Vol 337 (1) ◽  
pp. 25-39 ◽  
Author(s):  
A.J. van Vuuren ◽  
E. Appeldoorn ◽  
H. Odijk ◽  
S. Humbert ◽  
V. Moncollin ◽  
...  
Keyword(s):  
Dna Repair ◽  
Protein Complex ◽  
Partial Characterization ◽  
Repair Protein ◽  
Dna Repair Protein

Identification and Characterization of the rhp23+ DNA Repair Gene in Schizosaccharomyces pombe

2000 ◽  
Vol 268 (1) ◽  
pp. 210-215 ◽  
Author(s):  
Marcel Lombaerts ◽  
Jerrelyne I. Goeloe ◽  
Hans den Dulk ◽  
Jourica A. Brandsma ◽  
Jaap Brouwer
Keyword(s):  
Dna Repair ◽  
Schizosaccharomyces Pombe ◽  
Repair Gene ◽  
Dna Repair Gene ◽  
Identification And Characterization

Characterization of the DNA repair spore photoproduct lyase enzyme from Clostridium acetobutylicum: A radical-SAM enzyme

2007 ◽  
Vol 10 (8) ◽  
pp. 756-765 ◽  
Author(s):  
Alexia Chandor ◽  
Thierry Douki ◽  
Didier Gasparutto ◽  
Serge Gambarelli ◽  
Yannis Sanakis ◽  
...  
Keyword(s):  
Dna Repair ◽  
Clostridium Acetobutylicum ◽  
Radical Sam ◽  
Spore Photoproduct Lyase ◽  
Radical Sam Enzyme ◽  
Spore Photoproduct

scholarly journals Characterization of the interplay between DNA repair and CRISPR/Cas9-induced DNA lesions at an endogenous locus

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Anne Bothmer ◽  
Tanushree Phadke ◽  
Luis A. Barrera ◽  
Carrie M Margulies ◽  
Christina S. Lee ◽  
...  
Keyword(s):  
Dna Repair ◽  
Dna Lesions ◽  
Endogenous Locus

Characterization of a Mutant RecA Protein that Facilitates Homologous Genetic Recombination but not Recombinational DNA Repair: RecA423

1996 ◽  
Vol 264 (4) ◽  
pp. 696-712 ◽  
Author(s):  
Koichiro Ishimori ◽  
Suzanne Sommer ◽  
Adriana Bailone ◽  
Masayuki Takahashi ◽  
Michael M. Cox ◽  
...  
Keyword(s):  
Dna Repair ◽  
Genetic Recombination ◽  
Reca Protein ◽  
Recombinational Dna Repair

Characterization of human polymorphic DNA repair methyltransferase

2000 ◽  
Vol 10 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Ryo Inoue ◽  
Masako Abe ◽  
Yusaku Nakabeppu ◽  
Mutsuo Sekiguchi ◽  
Teruaki Mori ◽  
...  
Keyword(s):  
Dna Repair

scholarly journals The Drosophila melanogaster Ortholog of RFWD3 Functions Independently of RAD51 During DNA Repair

2020 ◽  
Vol 10 (3) ◽  
pp. 999-1004
Author(s):  
Juan Carvajal-Garcia ◽  
Evan R. Gales ◽  
Dale A. Ramsden ◽  
Jeff Sekelsky
Keyword(s):  
Dna Repair ◽  
Drosophila Melanogaster ◽  
Ring Finger ◽  
Double Strand Breaks ◽  
Specific Gene ◽  
Genetic Screens ◽  
Strand Breaks ◽  
Ancestral Function ◽  
Human Ortholog

Repair of damaged DNA is required for the viability of all organisms. Studies in Drosophila melanogaster, driven by the power of genetic screens, pioneered the discovery and characterization of many genes and pathways involved in DNA repair in animals. However, fewer than half of the alleles identified in these screens have been mapped to a specific gene, leaving a potential for new discoveries in this field. Here we show that the previously uncharacterized mutagen sensitive gene mus302 codes for the Drosophila melanogaster ortholog of the E3 ubiquitin ligase RING finger and WD domain protein 3 (RFWD3). In human cells, RFWD3 promotes ubiquitylation of RPA and RAD51 to facilitate repair of collapsed replication forks and double-strand breaks through homologous recombination. Despite the high similarity in sequence to the human ortholog, our evidence fails to support a role for Mus302 in the repair of these types of damage. Last, we observe that the N-terminal third of RFWD3 is only found in mammals, but not in other vertebrates or invertebrates. We propose that the new N-terminal sequence accounts for the acquisition of a new biological function in mammals that explains the functional differences between the human and the fly orthologs, and that Drosophila Mus302 may retain the ancestral function of the protein.

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