Human microRNA clusters: Genomic organization and expression profile in leukemia cell lines

2006 ◽  
Vol 349 (1) ◽  
pp. 59-68 ◽  
Author(s):  
Jia Yu ◽  
Fang Wang ◽  
Gui-Hua Yang ◽  
Fan-Long Wang ◽  
Yan-Ni Ma ◽  
...  
Keyword(s):  
Cell Lines ◽  
Expression Profile ◽  
Genomic Organization ◽  
Leukemia Cell ◽  
Leukemia Cell Lines

scholarly journals Ampelopsin Inhibits Cell Proliferation and Induces Apoptosis in HL60 and K562 Leukemia Cells by Downregulating AKT and NF-κB Signaling Pathways

2021 ◽  
Vol 22 (8) ◽  
pp. 4265
Author(s):  
Jang Mi Han ◽  
Hong Lae Kim ◽  
Hye Jin Jung
Keyword(s):  
Signaling Pathways ◽  
Cell Lines ◽  
White Blood Cells ◽  
Leukemia Cell ◽  
Leukemia Cells ◽  
Ros Generation ◽  
Anticancer Activities ◽  
Nuclear Condensation ◽  
Leukemia Cell Lines ◽  
Antileukemic Effect

Leukemia is a type of blood cancer caused by the rapid proliferation of abnormal white blood cells. Currently, several treatment options, including chemotherapy, radiation therapy, and bone marrow transplantation, are used to treat leukemia, but the morbidity and mortality rates of patients with leukemia are still high. Therefore, there is still a need to develop more selective and less toxic drugs for the effective treatment of leukemia. Ampelopsin, also known as dihydromyricetin, is a plant-derived flavonoid that possesses multiple pharmacological functions, including antibacterial, anti-inflammatory, antioxidative, antiangiogenic, and anticancer activities. However, the anticancer effect and mechanism of action of ampelopsin in leukemia remain unclear. In this study, we evaluated the antileukemic effect of ampelopsin against acute promyelocytic HL60 and chronic myelogenous K562 leukemia cells. Ampelopsin significantly inhibited the proliferation of both leukemia cell lines at concentrations that did not affect normal cell viability. Ampelopsin induced cell cycle arrest at the sub-G1 phase in HL60 cells but the S phase in K562 cells. In addition, ampelopsin regulated the expression of cyclins, cyclin-dependent kinases (CDKs), and CDK inhibitors differently in each leukemia cell. Ampelopsin also induced apoptosis in both leukemia cell lines through nuclear condensation, loss of mitochondrial membrane potential, increase in reactive oxygen species (ROS) generation, activation of caspase-9, caspase-3, and poly ADP-ribose polymerase (PARP), and regulation of Bcl-2 family members. Furthermore, the antileukemic effect of ampelopsin was associated with the downregulation of AKT and NF-κB signaling pathways. Moreover, ampelopsin suppressed the expression levels of leukemia stemness markers, such as Oct4, Sox2, CD44, and CD133. Taken together, our findings suggest that ampelopsin may be an attractive chemotherapeutic agent against leukemia.

scholarly journals Quantitative proteomic analysis of histone modifications in decitabine sensitive and resistant leukemia cell lines

2016 ◽  
Vol 13 (1) ◽  
Author(s):  
Chunchao Zhang ◽  
Jinfeng Suo ◽  
Hiroyuki Katayama ◽  
Yue Wei ◽  
Guillermo Garcia-Manero ◽  
...  
Keyword(s):  
Cell Lines ◽  
Histone Modifications ◽  
Proteomic Analysis ◽  
Leukemia Cell ◽  
Quantitative Proteomic Analysis ◽  
Leukemia Cell Lines
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