One-step immobilization of poly(dT)-modified DNA onto non-modified plastic substrates by UV irradiation for microarrays

2006 ◽  
Vol 347 (2) ◽  
pp. 477-484 ◽  
Author(s):  
Naoki Kimura
Keyword(s):  
Uv Irradiation ◽  
Plastic Substrates ◽  
Modified Dna ◽  
One Step

Reduction of lignin color via one-step UV irradiation

2016 ◽  
Vol 18 (3) ◽  
pp. 695-699 ◽  
Author(s):  
Jingyu Wang ◽  
Yonghong Deng ◽  
Yong Qian ◽  
Xueqing Qiu ◽  
Yuan Ren ◽  
...  
Keyword(s):  
Uv Irradiation ◽  
One Step

One-step irradiation in a mild solvent caused lignin to be partially depolymerized and π–π disaggregated and thus lignin was largely whitened.

scholarly journals One-step selective-wettability modification of PMMA microfluidic devices by using controllable gradient UV irradiation (CGUI)

2018 ◽  
Vol 273 ◽  
pp. 1508-1518 ◽  
Author(s):  
Chao Liang ◽  
Yuanchang Liu ◽  
Chong Liu ◽  
Xia Li ◽  
Li Chen ◽  
...  
Keyword(s):  
Uv Irradiation ◽  
Microfluidic Devices ◽  
One Step

Effect of Reaction Time on the Morphology and Photocatalytic Property of ZnO Nanoparticles

2017 ◽  
Vol 726 ◽  
pp. 365-369
Author(s):  
Xia Kong ◽  
Ya Wei Hu ◽  
Wei Pan
Keyword(s):  
Aqueous Solution ◽  
Methylene Blue ◽  
Reaction Time ◽  
Uv Irradiation ◽  
Zno Nanoparticles ◽  
Uv Light ◽  
Photocatalytic Property ◽  
Crystallographic Structure ◽  
Elliptical Shape ◽  
One Step

Abstract. ZnO nanoparticles with different morphology were synthesized through a one-step and low temperature hydrothermal method with different reaction time. The prepared ZnO nanoparticles have been used as photocatalysts for the degradation of methylene blue (MB) aqueous solution under UV irradiation to study the relationship between the morphology and photocatalytic performance. The phase, crystallographic structure and morphology of synthesised ZnO nanoparticles were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD). The photocatalytic activity of ZnO nanoparticles were carried out by UV-visible spectroscopy (UV-vis). SEM results showed that different particle sizes and morphologies of flower-like, elliptical-shape and rod-shapes were obtained at 60 °C for 1 h, 4 h, 8 h and 12 h, which promoted photodegradation of methylene blue (MB) aqueous solution under UV light irradiation. Especially, elliptical-shape ZnOnanoparticles with reaction time of 4 h were most efficient, and the degradation rate was up to 98.2% after 20 min UV irradiation.

One-step generation of degraded DNA by UV irradiation

2007 ◽  
Vol 360 (1) ◽  
pp. 163-165 ◽  
Author(s):  
B.C.M. Pang ◽  
B.K.K. Cheung
Keyword(s):  
Uv Irradiation ◽  
Degraded Dna ◽  
One Step ◽  
Step Generation

scholarly journals Enhancement of Solvent Resistance of Polyimide Electrospun Mat via the UV-Assisted Electrospinning and Photosensitive Varnish

Polymers ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 2055 ◽  
Author(s):  
Lin Qi ◽  
Chen-Yu Guo ◽  
Meng-Ge Huang Fu ◽  
Yan Zhang ◽  
Lu-meng Yin ◽  
...  
Keyword(s):  
High Pressure ◽  
High Temperature ◽  
Uv Irradiation ◽  
Starting Material ◽  
Current Work ◽  
Mercury Lamp ◽  
Solvent Resistance ◽  
One Step ◽  
The One

A new methodology for enhancing the solvent resistance of electrospun polyimide (PI) ultrafine fibrous mat (UFM) was investigated in the current work. For this purpose, a negative intrinsically photosensitive polyimide (PSPI) resin was prepared by the one-step high- temperature polycondensation procedure from 3,3’,4,4’-benzophenonetetracarboxylic dianhydride (BTDA) and α,α-bis(4-amino-3,5-dimethylphenyl)phenylmethane (PTMDA). The PI varnish, by dissolving the derived PI (BTDA-PTMDA) resin in N,N-dimethylacetamide (DMAc) at a solid of 20 wt %, was used as the starting material for the standard electrospinning (ES) and ultraviolet-assisted ES (UVAES) fabrications, respectively. The 365 nm wavelength of the high-pressure mercury lamp ultraviolet (UV) irradiation induced the photocrosslinking reaction in the PSPI mat. Solubility tests indicated that the PI UFM fabricated by standard ES procedure showed poor DMAc resistance, while the one by UVAES (PI-UV) exhibited excellent resistance to DMAc.

One-step method for preparation of pH-responsive gold nanoparticles with block copolymer shell structures by UV irradiation

2011 ◽  
Vol 67 (6) ◽  
pp. 1059-1072 ◽  
Author(s):  
Xiaoning Fu ◽  
Hui Yang ◽  
Xiaokai Zhang ◽  
Xue Li ◽  
Limei Xu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Block Copolymer ◽  
Uv Irradiation ◽  
Shell Structures ◽  
Ph Responsive ◽  
Step Method ◽  
One Step

scholarly journals Photoswitching of Site-Selective RNA Scission by Sequential Incorporation of Azobenzene and Acridine Residues in a DNA Oligomer

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Akinori Kuzuya ◽  
Keita Tanaka ◽  
Makoto Komiyama
Keyword(s):  
Uv Irradiation ◽  
Intrinsic Activity ◽  
Modified Dna ◽  
Mixture Prior ◽  
Site Selective ◽  
Cis Isomer

Photoresponsive systems for site-selective RNA scission have been prepared by combining Lu(III) ions with acridine/azobenzene dual-modified DNA. The modified DNA forms a heteroduplex with substrate RNA, and the target phosphodiester linkages in front of the acridine residue is selectively activated so that Lu(III) ion rapidly cleaves the linkage. Azobenzene residue introduced adjacent to the acridine residue acts as a photoresponsive switch, which triggers the site-selective scission upon UV irradiation. Atransisomer of azobenzene efficiently suppresses the scission, whereas the cis isomer formed by UV irradiation hardly affects the scission. As a result, 1.7–2.4-fold acceleration of the cleavage was achieved simply by irradiating UV for 3 min to the mixture prior to the reaction. Considering the yield of photoisomerization, the intrinsic activity of acisisomer is up to 14.5-fold higher than that of thetransisomer.

scholarly journals Automated SARS-COV-2 RNA extraction from patient nasopharyngeal samples using a modified DNA extraction kit for high throughput testing

2020 ◽  
Vol 40 (5) ◽  
pp. 373-381
Author(s):  
Haya Al-Saud ◽  
Khaldoun Al-Romaih ◽  
Razan Bakheet ◽  
Lina Mahmoud ◽  
Najla Al-Harbi ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Rna Virus ◽  
Rna Extraction ◽  
Viral Rna ◽  
Modified Dna ◽  
One Step ◽  
Small Patient ◽  
Polymerase Chain ◽  
Probe Assay ◽  
Commercial Kit

ABSTRACT BACKGROUND: The pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) has prompted a need for mass testing to identify patients with viral infection. The high demand has created a global bottleneck in testing capacity, which prompted us to modify available resources to extract viral RNA and perform reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) to detect SARS-COV-2. OBJECTIVES: Report on the use of a DNA extraction kit, after modifications, to extract viral RNA that could then be detected using an FDA-approved SARS-COV-2 RT-qPCR assay. MATERIALS AND METHODS: Initially, automated RNA extraction was performed using a modified DNA kit on samples from control subjects, a bacteriophage, and an RNA virus. We then verified the automated extraction using the modified kit to detect in-lab propagated SARSCOV-2 titrations using an FDA approved commercial kit (S, N, and ORF1b genes) and an in-house primer-probe based assay (E, RdRp2 and RdRp4 genes). RESULTS: Automated RNA extraction on serial dilutions SARS-COV-2 achieved successful one-step RT-qPCR detection down to 60 copies using the commercial kit assay and less than 30 copies using the in-house primer-probe assay. Moreover, RT-qPCR detection was successful after automated RNA extraction using this modified protocol on 12 patient samples of SARS-COV-2 collected by nasopharyngeal swabs and stored in viral transport media. CONCLUSIONS: We demonstrated the capacity of a modified DNA extraction kit for automated viral RNA extraction and detection using a platform that is suitable for mass testing. LIMITATIONS: Small patient sample size. CONFLICT OF INTEREST: None.

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