Small interfering RNAs generated by recombinant dicer induce inflammatory gene expression independent from the TAK1-NFκB-MAPK signaling pathways

2006 ◽  
Vol 347 (3) ◽  
pp. 566-573 ◽  
Author(s):  
Daniela Kettner-Buhrow ◽  
Oliver Dittrich-Breiholz ◽  
Heike Schneider ◽  
Sabine Wolter ◽  
Klaus Resch ◽  
...  
Keyword(s):  
Gene Expression ◽  
Signaling Pathways ◽  
Mapk Signaling ◽  
Small Interfering Rnas ◽  
Inflammatory Gene Expression ◽  
Inflammatory Gene ◽  
Mapk Signaling Pathways

scholarly journals Identification of multiple MAPK-mediated transcription factors regulated by tobacco smoke in airway epithelial cells

2007 ◽  
Vol 293 (2) ◽  
pp. L480-L490 ◽  
Author(s):  
Jinming Zhao ◽  
Richart Harper ◽  
Aaron Barchowsky ◽  
Y. P. Peter Di
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Dna Binding ◽  
Signaling Pathways ◽  
Tobacco Smoke ◽  
Mapk Signaling ◽  
Regulation Of Transcription ◽  
Inflammatory Gene Expression ◽  
Inflammatory Gene ◽  
Mapk Signaling Pathways

Activation and regulation of transcription factors (TFs) are the major mechanisms regulating changes in gene expression upon environmental exposure. Tobacco smoke (TS) is a complex mixture of chemicals, each of which could act through different signal cascades, leading to the regulation of distinct TFs and alterations in subsequent gene expression. We proposed that TS exposure affects inflammatory gene expression at the transcriptional level by modulating the DNA binding activities of TFs. To investigate transcriptional regulation upon TS exposure, a protein/DNA array was applied to screen TFs that are affected by TS exposure. This array-based screening allowed us to simultaneously detect 244 different TFs. Our results indicated that multiple TFs were rapidly activated upon TS exposure. DNA-binding activity of differentially expressed TFs was confirmed by EMSA. Our results showed that at least 20 TFs displayed more than twofold expressional changes after smoke treatment. Ten smoke-induced TFs, including NF-κB, VDR, ISRE, and RSRFC4, were involved in MAPK signaling pathways. The NF-κB family, which is involved in inflammation-induced gene activation, was selected for further study to characterize TS exposure-induced transcriptional activation. Western blot analysis and immunofluorescence microscopy indicated that TS exposure induced phosphorylation of IκB and translocation of NF-κB p65/p50 heterodimers into the nucleus. This activity was abrogated by the MAPK inhibitors PD98059 and U0126. Our results confirmed that activation of MAPK signaling pathways by TS exposure increased transcriptional activity of NF-κB. These data provide a potential mechanism for TS-induced inflammatory gene expression.

scholarly journals Defined Sensing Mechanisms and Signaling Pathways Contribute to the Global Inflammatory Gene Expression Output Elicited by Ionizing Radiation

Immunity ◽  
2017 ◽  
Vol 47 (3) ◽  
pp. 421-434.e3 ◽  
Author(s):  
Prabhat K. Purbey ◽  
Philip O. Scumpia ◽  
Peter J. Kim ◽  
Ann-Jay Tong ◽  
Keisuke S. Iwamoto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ionizing Radiation ◽  
Signaling Pathways ◽  
Inflammatory Gene Expression ◽  
Inflammatory Gene

scholarly journals Effects of Deoxynivalenol and Mycotoxin Adsorbent Agents on Mitogen-Activated Protein Kinase Signaling Pathways and Inflammation-Associated Gene Expression in Porcine Intestinal Epithelial Cells

Toxins ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 301
Author(s):  
Yu-Hsiang Yu ◽  
Yi-Han Lai ◽  
Felix Shih-Hsiang Hsiao ◽  
Yeong-Hsiang Cheng
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Signaling Pathways ◽  
Inflammatory Responses ◽  
Intestinal Epithelial Cells ◽  
Mapk Signaling ◽  
Mitogen Activated Protein Kinase ◽  
Intestinal Epithelial ◽  
Mycotoxin Adsorbent ◽  
Mapk Signaling Pathways

Deoxynivalenol (DON) is the most prevalent mycotoxin in swine feedstuffs. The intestinal epithelial cells represent the first target for the DON. Here, we studied the effects of DON and mycotoxin adsorbent agents on mitogen-activated protein kinase (MAPK) signaling pathways and inflammation-associated gene expression in porcine intestinal epithelial cells (IPEC-J2). Results showed that phosphorylation of MAPK signaling pathways (p38, ERK, and JNK) was increased after treatment of DON or lipopolysaccharide (LPS) in IPEC-J2 cells. The phosphorylation of p38, ERK, and JNK was not further enhanced after co-treatment with DON and LPS. The inos and cox-2 mRNA expression were significantly induced at 6 h after treatment of DON. DON treatment significantly increased the claudin 3 and occludin mRNA expression at 12 h. DON in combination with LPS treatment did not further increase the inflammation and tight junction-associated gene expression. The DON-induced phosphorylation of MAPK signaling pathways was impaired by mycotoxin adsorbent agent (nanoscale silicate platelets and the mixture of montmorillonites and yeast cell walls) treatment, thereby decreasing inflammation and tight junction-associated gene expression. Taken together, these findings demonstrate that DON triggers the inflammation in IPEC-J2 cells by phosphorylation of MAPK signaling pathways and LPS does not further augment the DON-induced inflammatory responses. Mycotoxin adsorbent agents can attenuate DON-induced inflammatory responses in IPEC-J2 cells through modulation of the phosphorylation of p38, ERK, and JNK.

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