Mutation D104G in ANT1 gene: Complementation study in Saccharomyces cerevisiae as a model system

2006 ◽  
Vol 341 (3) ◽  
pp. 810-815 ◽  
Author(s):  
Tiziana Lodi ◽  
Claudio Bove ◽  
Flavia Fontanesi ◽  
Anna Maria Viola ◽  
Iliana Ferrero
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Model System ◽  
Gene Complementation ◽  
Complementation Study

An examination of quinone toxicity using the yeast Saccharomyces cerevisiae model system

Toxicology ◽  
2004 ◽  
Vol 201 (1-3) ◽  
pp. 185-196 ◽  
Author(s):  
Chester E Rodriguez ◽  
Masaru Shinyashiki ◽  
John Froines ◽  
Rong Chun Yu ◽  
Jon M Fukuto ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Model System ◽  
Yeast Saccharomyces Cerevisiae ◽  
Quinone Toxicity

scholarly journals Saccharomyces cerevisiae as a Tool to Investigate Plant Potassium and Sodium Transporters

2019 ◽  
Vol 20 (9) ◽  
pp. 2133 ◽  
Author(s):  
Antonella Locascio ◽  
Nuria Andrés-Colás ◽  
José Miguel Mulet ◽  
Lynne Yenush
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Interactions ◽  
Model System ◽  
Protein Protein Interactions ◽  
Alkali Cations ◽  
Yeast Saccharomyces Cerevisiae ◽  
Future Directions ◽  
Sodium Transporters ◽  
Adverse Environmental Conditions ◽  
Sodium And Potassium

Sodium and potassium are two alkali cations abundant in the biosphere. Potassium is essential for plants and its concentration must be maintained at approximately 150 mM in the plant cell cytoplasm including under circumstances where its concentration is much lower in soil. On the other hand, sodium must be extruded from the plant or accumulated either in the vacuole or in specific plant structures. Maintaining a high intracellular K+/Na+ ratio under adverse environmental conditions or in the presence of salt is essential to maintain cellular homeostasis and to avoid toxicity. The baker’s yeast, Saccharomyces cerevisiae, has been used to identify and characterize participants in potassium and sodium homeostasis in plants for many years. Its utility resides in the fact that the electric gradient across the membrane and the vacuoles is similar to plants. Most plant proteins can be expressed in yeast and are functional in this unicellular model system, which allows for productive structure-function studies for ion transporting proteins. Moreover, yeast can also be used as a high-throughput platform for the identification of genes that confer stress tolerance and for the study of protein–protein interactions. In this review, we summarize advances regarding potassium and sodium transport that have been discovered using the yeast model system, the state-of-the-art of the available techniques and the future directions and opportunities in this field.

Grafting of gold complex on nano-porous MCM-41 and evaluation of its toxicity in Saccharomyces cerevisiae as a model system

2011 ◽  
Vol 44 (13) ◽  
pp. S363
Author(s):  
Yousef Fazaeli ◽  
Mostafa M. Amini ◽  
Hamed Ashoorion ◽  
Amirreza Jalilian ◽  
Abbas Majdabadi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Model System ◽  
Gold Complex ◽  
Mcm 41

Saccharomyces cerevisiae as a model system to study the response to anticancer agents

2012 ◽  
Vol 70 (4) ◽  
pp. 491-502 ◽  
Author(s):  
Renata Matuo ◽  
Fabrício G. Sousa ◽  
Daniele G. Soares ◽  
Diego Bonatto ◽  
Jenifer Saffi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Anticancer Agents ◽  
Model System

scholarly journals Saccharomyces cerevisiae pms2 mutations are alleles of MLH1, and pms2-2 corresponds to a hereditary nonpolyposis colorectal carcinoma-causing missense mutation.

1996 ◽  
Vol 16 (6) ◽  
pp. 3008-3011 ◽  
Author(s):  
A Jeyaprakash ◽  
R Das Gupta ◽  
R Kolodner
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
Colorectal Carcinoma ◽  
Missense Mutation ◽  
Model System ◽  
Missense Mutations ◽  
Mlh1 Gene ◽  
Dna Mismatch ◽  
Hereditary Nonpolyposis ◽  
Hereditary Nonpolyposis Colorectal Carcinoma

A number of mutant Saccharomyces cerevisiae strains having phenotypes consistent with defects in DNA mismatch repair have been described, but not all have been extensively characterized. In this study we demonstrate that the pms2-1 and pms2-2 alleles arise from missense mutations in the MLH1 gene which inactivate MLH1. One of these alleles, pms2-2, causes the same amino acid substitution in a highly conserved region of the known MutL homologs as that caused by a proposed missense mutation observed in a Swedish hereditary nonpolyposis colorectal carcinoma kindred. This observation supports the functional significance of missense mutations found in hereditary nonpolyposis colorectal carcinoma kindreds and indicates that in some cases S. cerevisiae can serve as a useful model system for the analysis of such mutations.

scholarly journals Integration of an insertion-type transferred DNA vector from Agrobacterium tumefaciens into the Saccharomyces cerevisiae genome by gap repair.

1996 ◽  
Vol 16 (10) ◽  
pp. 5924-5932 ◽  
Author(s):  
E Risseeuw ◽  
M E Franke-van Dijk ◽  
P J Hooykaas
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Agrobacterium Tumefaciens ◽  
Homologous Recombination ◽  
Model System ◽  
Transformation Frequency ◽  
Yeast Genome ◽  
Repair Mechanism ◽  
Replacement Vector ◽  
Dna Vector ◽  
Yeast Transformants

Recently, it was shown that Agrobacterium tumefaciens can transfer transferred DNA (T-DNA) to Saccharomyces cerevisiae and that this T-DNA, when used as a replacement vector, is integrated via homologous recombination into the yeast genome. To test whether T-DNA can be a suitable substrate for integration via the gap repair mechanism as well, a model system developed for detection of homologous recombination events in plants was transferred to S. cerevisiae. Analysis of the yeast transformants revealed that an insertion type T-DNA vector can indeed be integrated via gap repair. Interestingly, the transformation frequency and the type of recombination events turned out to depend strongly on the orientation of the insert between the borders in such an insertion type T-DNA vector.

scholarly journals Relations between Effects and Structure of Small Bicyclic Molecules on the Complex Model System Saccharomyces cerevisiae

2017 ◽  
Vol 8 ◽  
Author(s):  
Matteo Brilli ◽  
Andrea Trabocchi ◽  
Tobias Weil ◽  
Duccio Cavalieri ◽  
Irene Stefanini
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Model System ◽  
Complex Model ◽  
Bicyclic Molecules

scholarly journals Recombination of plasmids into the Saccharomyces cerevisiae chromosome is reduced by small amounts of sequence heterogeneity.

1983 ◽  
Vol 3 (7) ◽  
pp. 1204-1211 ◽  
Author(s):  
S Smolik-Utlaut ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Sequences ◽  
Recombinant Plasmid ◽  
Mitotic Recombination ◽  
Model System ◽  
Rrna Genes ◽  
Yeast Saccharomyces Cerevisiae ◽  
Sequence Heterogeneity ◽  
Recombinant Plasmids ◽  
Chromosomal Loci

As a model system for studying the properties of mitotic recombination in the yeast Saccharomyces cerevisiae, we have examined recombination between a recombinant plasmid (introduced into the S. cerevisiae cell by transformation) and homologous chromosomal loci. The recombinant plasmids used in these experiments contained S. cerevisiae rRNA genes. We found that the frequency of integrative recombination is sensitive to small amounts of sequence heterogeneity. In addition, the frequency and specificity of these recombination events are affected by the lengths of the interacting homologous DNA sequences.

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