scholarly journals Identification of 5′-upstream region of pufferfish ribosomal protein L29 gene as a strong constitutive promoter to drive GFP expression in zebrafish

2004 ◽  
Vol 314 (1) ◽  
pp. 249-258 ◽  
Author(s):  
Ming-Huang Chang ◽  
Chih-Ming Chou ◽  
Yueh-Chun Hsieh ◽  
I-Ching Lu ◽  
M.Kothai Nachiar Devi ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Upstream Region ◽  
Constitutive Promoter ◽  
Gfp Expression

The upstream region of the mouse Xist gene contains two ribosomal protein pseudogenes

2000 ◽  
Vol 11 (6) ◽  
pp. 461-463 ◽  
Author(s):  
Justyna T. Romer ◽  
Alan Ashworth
Keyword(s):  
Ribosomal Protein ◽  
Xist Gene ◽  
Upstream Region

scholarly journals Eubacterial Diterpene Cyclase Genes Essential for Production of the Isoprenoid Antibiotic Terpentecin

2001 ◽  
Vol 183 (20) ◽  
pp. 6085-6094 ◽  
Author(s):  
Tohru Dairi ◽  
Yoshimitsu Hamano ◽  
Tomohisa Kuzuyama ◽  
Nobuya Itoh ◽  
Kazuo Furihata ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Analysis ◽  
Mevalonate Pathway ◽  
Open Reading Frame ◽  
Upstream Region ◽  
Constitutive Promoter ◽  
Isopentenyl Diphosphate ◽  
Geranylgeranyl Diphosphate ◽  
Reading Frame ◽  
Open Reading Frame 2

ABSTRACT A gene cluster containing the mevalonate pathway genes (open reading frame 2 [ORF2] to ORF7) for the formation of isopentenyl diphosphate and a geranylgeranyl diphosphate (GGDP) synthase gene (ORF1) had previously been cloned from Streptomyces griseolosporeus strain MF730-N6, a diterpenoid antibiotic, terpentecin (TP) producer (Y. Hamano, T. Dairi, M. Yamamoto, T. Kawasaki, K Kaneda, T. Kuzuyama, N. Itoh, and H. Seto, Biosci. Biotech. Biochem. 65:1627–1635, 2001). Sequence analysis in the upstream region of the cluster revealed seven new ORFs, ORF8 to ORF14, which were suggested to encode TP biosynthetic genes. We constructed two mutants, in which ORF11 and ORF12, which encode a protein showing similarities to eukaryotic diterpene cyclases (DCs) and a eubacterial pentalenene synthase, respectively, were inactivated by gene disruptions. The mutants produced no TP, confirming that these cyclase genes are essential for the production of TP. The two cyclase genes were also expressed in Streptomyces lividans together with the GGDP synthase gene under the control of theermE* constitutive promoter. The transformant produced a novel cyclic diterpenoid, ent-clerod-3,13(16),14-triene (terpentetriene), which has the same basic skeleton as TP. The two enzymes, each of which was overproduced in Escherichia coli and purified to homogeneity, converted GGDP into terpentetriene. To the best of our knowledge, this is the first report of a eubacterial DC.

scholarly journals Functional characterization of transcriptional regulatory elements in the upstream region and intron 1 of the human S6 ribosomal protein gene

1998 ◽  
Vol 336 (2) ◽  
pp. 327-335 ◽  
Author(s):  
Marianne ANTOINE ◽  
Paul KIEFER
Keyword(s):  
Ribosomal Protein ◽  
Specific Binding ◽  
Core Promoter ◽  
Functional Characterization ◽  
Housekeeping Genes ◽  
Regulatory Elements ◽  
Upstream Region ◽  
Splice Donor Site ◽  
The Core ◽  
Intron 1

Expression of housekeeping genes involves regulation at comparable levels in a wide spectrum of cells. To define the cis-regulatory elements in the human S6 ribosomal protein (rpS6) gene, we made a series of deletions of the upstream non-transcribed region, including or excluding exon 1 or intron 1 sequences. The mutated rpS6 gene regulatory regions were fused to the chloramphenicol acetyltransferase reporter gene and transfected into HeLa and COS-1 cells. The results have identified three parts of the rpS6 gene that are required for efficient and specific transcription. The core promoter includes only a 40 bp region upstream of the transcription start site and initiation region. Both upstream and intronic elements enhance transcription from the core promoter. Furthermore, mutation of the splice donor site of intron 1 almost completely abolished the enhancing activity of the intronic transcriptional modulator. We used gel retardation assays to identify sequence-specific binding sites in the upstream region and in the proximal half of intron 1. Both common and different nuclear factors that bind the rpS6 gene promoter were identified in extracts from HeLa and COS-1 cells, suggesting that different transcription factors may bind specifically to the same binding region and might be interchangeable in their function to ensure high-level expression of housekeeping genes independently of the cell type.

scholarly journals The rcsA Promoter of Pantoea stewartii subsp. stewartii Features a Low-Level Constitutive Promoter and an EsaR Quorum-Sensing-Regulated Promoter

2006 ◽  
Vol 188 (12) ◽  
pp. 4581-4584 ◽  
Author(s):  
Aurelien L. Carlier ◽  
S. B. von Bodman
Keyword(s):  
Quorum Sensing ◽  
Transcription Elongation ◽  
Upstream Region ◽  
Constitutive Promoter ◽  
Induced Expression ◽  
Low Level ◽  
Pantoea Stewartii ◽  
A Site ◽  
Basal Level Expression

ABSTRACT The upstream region of the Pantoea stewartii rcsA gene features two promoters, one for constitutive basal-level expression and a second autoregulated promoter for induced expression. The EsaR quorum-sensing repressor binds to a site centered between the two promoters, blocking transcription elongation from the regulated promoter under noninducing conditions.

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