Human adipose-derived stem cells ameliorate repetitive behavior, social deficit and anxiety in a VPA-induced autism mouse model

2017 ◽  
Vol 317 ◽  
pp. 479-484 ◽  
Author(s):  
Sungji Ha ◽  
Hyunjun Park ◽  
Usman Mahmood ◽  
Jeong Chan Ra ◽  
Yoo-Hun Suh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mouse Model ◽  
Repetitive Behavior ◽  
Adipose Derived Stem Cells ◽  
Social Deficit

scholarly journals Therapeutic Potential of Magnetic Nanoparticle-Based Human Adipose-Derived Stem Cells in a Mouse Model of Parkinson’s Disease

2021 ◽  
Vol 22 (2) ◽  
pp. 654
Author(s):  
Ka Young Kim ◽  
Keun-A Chang
Keyword(s):  
Parkinson’S Disease ◽  
Stem Cells ◽  
Parkinson's Disease ◽  
Magnetic Nanoparticles ◽  
Substantia Nigra ◽  
Mouse Model ◽  
Magnetic Nanoparticle ◽  
Imaging System ◽  
Therapeutic Potential ◽  
Adipose Derived Stem Cells

Parkinson’s disease (PD) is a progressive neurodegenerative disease characterized by the loss of dopaminergic neurons in the substantia nigra. Several treatments for PD have focused on the management of physical symptoms using dopaminergic agents. However, these treatments induce various adverse effects, including hallucinations and cognitive impairment, owing to non-targeted brain delivery, while alleviating motor symptoms. Furthermore, these therapies are not considered ultimate cures owing to limited brain self-repair and regeneration abilities. In the present study, we aimed to investigate the therapeutic potential of human adipose-derived stem cells (hASCs) using magnetic nanoparticles in a 6-hydroxydopamine (6-OHDA)-induced PD mouse model. We used the Maestro imaging system and magnetic resonance imaging (MRI) for in vivo tracking after transplantation of magnetic nanoparticle-loaded hASCs to the PD mouse model. The Maestro imaging system revealed strong hASCs signals in the brains of PD model mice. In particular, MRI revealed hASCs distribution in the substantia nigra of hASCs-injected PD mice. Behavioral evaluations, including apomorphine-induced rotation and rotarod performance, were significantly recovered in hASCs-injected 6-OHDA induced PD mice when compared with saline-treated counterparts. Herein, we investigated whether hASCs transplantation using magnetic nanoparticles recovered motor functions through targeted brain distribution in a 6-OHDA induced PD mice. These results indicate that magnetic nanoparticle-based hASCs transplantation could be a potential therapeutic strategy in PD.

Extracellular vesicles derived from adipose-derived stem cells accelerate diabetic wound healing by suppressing the expression of matrix metalloproteinase-9

2021 ◽  
Vol 22 ◽  
Author(s):  
Jiang-wen Wang ◽  
Yuan-zheng Zhu ◽  
Xuan Hu ◽  
Jia-ying Nie ◽  
Zhao-hui Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mouse Model ◽  
Adipose Derived Stem Cells ◽  
Collagen Deposition ◽  
Diabetic Wound ◽  
Diabetic Wound Healing ◽  
Diabetic Wounds ◽  
Metalloproteinase 9

Background: The healing of diabetic wounds is poor due to a collagen deposition disorder. Matrix metalloproteinase-9 (MMP-9) is closely related to collagen deposition in the process of tissue repair. Many studies have demonstrated that extracellular vesicles derived from adipose-derived stem cells (ADSC-EVs) promote diabetic wound healing by enhancing collagen deposition. Objective: In this study, we explored if ADSC-EVs could downregulate the expression of MMP-9 in diabetic wounds and promote wound healing by improving collagen deposition. The potential effects of ADSC-EVs on MMP-9 and diabetic wound healing were tested both in vitro and in vivo. Methods: We first evaluated the effect of ADSC-EVs on the proliferation and MMP-9 secretion of HaCaT cells treated with advanced glycation end product-bovine serum albumin (AGE-BSA), using CCK-8 western blot and MMP-9 enzyme-linked immunosorbent assay(ELISA). Next, the effect of ADSC-EVs on the healing, re-epithelialisation, collagen deposition, and MMP-9 concentration in diabetic wound fluids was evaluated in an immunodeficient mouse model via MMP-9 ELISA and haematoxylin and eosin, Masson’s trichrome, and immunofluorescence staining for MMP-9. Results: In vitro, ADSC-EVs promoted the proliferation and MMP-9 secretion of HaCaT cells.In vivo, ADSC-EVs accelerated diabetic wound healing by improving re-epithelialisation and collagen deposition and by inhibiting the expression of MMP-9. Conclusion: ADSC-EVs possessed the healing of diabetic wounds in a mouse model by inhibiting downregulating MMP-9 and improving collagen deposition.Thus ,ADSC-EVs are a promising candidate for the treatment of diabetic wounds .

An electrochemically deposited collagen wound matrix combined with adipose-derived stem cells improves cutaneous wound healing in a mouse model of type 2 diabetes

2018 ◽  
Vol 33 (4) ◽  
pp. 553-565 ◽  
Author(s):  
Nicole Edwards ◽  
Denis Feliers ◽  
Qingwei Zhao ◽  
Randolph Stone ◽  
Robert Christy ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Stem Cells ◽  
Mouse Model ◽  
Chronic Wounds ◽  
High Porosity ◽  
Adipose Derived Stem Cells ◽  
Clinical Issue ◽  
Wound Matrix ◽  
Electrochemically Deposited

Chronic wounds complicated by diabetes are a significant clinical issue, and their occurrence is expected to continue to rise due to an increased prevalence of diabetes mellitus, especially type 2 diabetes. Diabetic wounds frequently lead to nonhealing ulcers, and often eventually result in limb amputation due to the high risk of infection of the chronic wound. Here, we present a tissue-engineered treatment that combines a novel electrochemically deposited collagen wound matrix and human adipose-derived stem cells. The matrix fabrication process is optimized for voltage and time, and the final collagen biomaterial is thoroughly characterized. This collagen material possesses high tensile strength, high porosity, and excellent biocompatibility and cellular proliferation capabilities. Human adipose-derived stem cells were seeded onto the collagen wound matrix and this construct is investigated in a full thickness excisional wound in a mouse model of type 2 diabetes. This novel treatment is shown to stimulate excellent healing and tissue regeneration, resulting in increased granulation tissue formation, epidermal thickness, and overall higher quality tissue reformation. Both the collagen wound matrix alone and collagen wound matrix in combination with adipose derived stem cells appeared to be excellent treatments for diabetic skin wounds, and in the future can also be optimized to treat other injuries such as burns, blast injuries, surgical incisions, and other traumatic injuries.

scholarly journals Anti-Aging Effect of Adipose-Derived Stem Cells in a Mouse Model of Skin Aging Induced by D-Galactose

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e97573 ◽  
Author(s):  
Shengchang Zhang ◽  
Ziqing Dong ◽  
Zhangsong Peng ◽  
Feng Lu
Keyword(s):  
Stem Cells ◽  
Mouse Model ◽  
Aging Effect ◽  
Skin Aging ◽  
Adipose Derived Stem Cells

scholarly journals Adipose Derived Stem Cells Ameliorate Atopic Dermatitis by Down-regulating IL-17 Secretion of Th17 Cells in an Ovalbumin-induced Mouse Model

2021 ◽  
Author(s):  
Jingyan Guan ◽  
Yibao Li ◽  
Feng Lu ◽  
Jingwei Feng
Keyword(s):  
Stem Cells ◽  
Atopic Dermatitis ◽  
Mouse Model ◽  
Therapeutic Effect ◽  
Th17 Cells ◽  
Therapeutic Potential ◽  
In Vitro Assays ◽  
Adipose Derived Stem Cells ◽  
Bioinformatics Analyses ◽  
Underlying Mechanisms

Abstract Background: Mesenchymal stem cells (MSCs) has therapeutic potential for Atopic dermatitis (AD) due to their immunoregulatory effects. However, the underlying mechanisms for therapeutic efficacy of ADSCs on AD is still unclear. Objectives: We sought to investigate the therapeutic effect and mechanisms of adipose-derived stem cells (ADSCs) on AD using an ovalbumin-induced AD mouse model. Methods: AD mice were treated with mice-derived ADSCs, cortisone, or PBS. The therapeutic effect was determined via gross examination and additional in vitro assays using skin samples and blood. To further explore the underlying mechanisms, RNA sequencing analyses and co-culture assays were conducted.Results: ADSCs treatment attenuated the symptoms associated with AD, decreased the serum IgE level and mast cells infiltration. Tissue levels of T-cell relevant pro-inflammatory cytokine production, including IL-4R and IL-17A, were suppressed in both ADSCs and cortisone treatment groups. Genomics and bioinformatics analyses demonstrated a significant enrichment of certain of inflammation related pathway in the down regulated genes after the application of ADSCs and cortisone, specifically the IL-17 signaling pathway. Co-culture experiments revealed that ADSCs significantly suppressed the expression of pro-inflammation cytokines IL-17A and RORγT, as well as the proliferation of Th17 cells. Moreover, the expression of PD-L1, TGF-β, PGE2 was significantly upregulated in the co-cultured ADSCs compare to monocultured ADSCs. Conclusion: Taken together, our data may demonstrate that ADSCs ameliorate OVA- induced AD in mice by down-regulating IL-17 secretion of Th17 cells.

Sign in / Sign up

Export Citation Format

Share Document