scholarly journals Brief isoflurane anaesthesia affects differential gene expression, gene ontology and gene networks in rat brain

2017 ◽  
Vol 317 ◽  
pp. 453-460 ◽  
Author(s):  
Damon A. Lowes ◽  
Helen F. Galley ◽  
Alessandro P.S. Moura ◽  
Nigel R. Webster
Keyword(s):  
Gene Expression ◽  
Gene Ontology ◽  
Rat Brain ◽  
Differential Gene Expression ◽  
Gene Networks ◽  
Isoflurane Anaesthesia ◽  
Differential Gene

PCR-Based Technologies to Study Differential Gene Expression in Rat Brain

1994 ◽  
pp. 261-271
Author(s):  
Mark G. Erlander ◽  
Ana Dopazo ◽  
Pamela E. Foye ◽  
J. Gregor Sutcliffe
Keyword(s):  
Gene Expression ◽  
Rat Brain ◽  
Differential Gene Expression ◽  
Differential Gene

scholarly journals The Western Diet Regulates Hippocampal Microvascular Gene Expression: An Integrated Genomic Analyses in Female Mice

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Saivageethi Nuthikattu ◽  
Dragan Milenkovic ◽  
John Rutledge ◽  
Amparo Villablanca
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Differential Expression ◽  
Differential Gene Expression ◽  
Gene Networks ◽  
Molecular Mechanisms ◽  
Western Diet ◽  
Protein Coding ◽  
Female Mice ◽  
Differential Gene

AbstractHyperlipidemia is a risk factor for dementia, and chronic consumption of a Western Diet (WD) is associated with cognitive impairment. However, the molecular mechanisms underlying the development of microvascular disease in the memory centers of the brain are poorly understood. This pilot study investigated the nutrigenomic pathways by which the WD regulates gene expression in hippocampal brain microvessels of female mice. Five-week-old female low-density lipoprotein receptor deficient (LDL-R−/−) and C57BL/6J wild type (WT) mice were fed a chow or WD for 8 weeks. Metabolics for lipids, glucose and insulin were determined. Differential gene expression, gene networks and pathways, transcription factors, and non-protein coding RNAs were evaluated by genome-wide microarray and bioinformatics analysis of laser captured hippocampal microvessels. The WD resulted in differential expression of 2,412 genes. The majority of differential gene expression was attributable to differential regulation of cell signaling proteins and their transcription factors, approximately 7% was attributable to differential expression of miRNAs, and a lesser proportion was due to other non-protein coding RNAs, primarily long non-coding RNAs (lncRNAs) and small nucleolar RNAs (snoRNAs) not previously described to be modified by the WD in females. Our findings revealed that chronic consumption of the WD resulted in integrated multilevel molecular regulation of the hippocampal microvasculature of female mice and may provide one of the mechanisms underlying vascular dementia.

Transcriptome Analysis Reveals Sexual Disparities in Differential Gene Expression Signature in Rat Brain Microvessels

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Partha K. Chandra ◽  
Sinisa Cikic ◽  
Melody C. Baddoo ◽  
Ibolya Rutkai ◽  
Erik K. Flemington ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Brain ◽  
Differential Gene Expression ◽  
Transcriptome Analysis ◽  
Gene Expression Signature ◽  
Expression Signature ◽  
Brain Microvessels ◽  
Differential Gene

scholarly journals Combined stress of ocean acidification and warming influence survival and drives differential gene expression patterns in the Antarctic pteropod, Limacina helicina antarctica

2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Kevin M Johnson ◽  
Gretchen E Hofmann
Keyword(s):  
Gene Expression ◽  
Ocean Acidification ◽  
Differential Gene Expression ◽  
Gene Networks ◽  
Expression Patterns ◽  
Significant Degree ◽  
Change Factors ◽  
Limacina Helicina ◽  
Differential Gene ◽  
The Antarctic

Abstract The ecologically important thecosome pteropods in the Limacina spp. complex have recently been the focus of studies examining the impacts global change factors – e.g., ocean acidification (OA) and ocean warming (OW) – on their performance and physiology. This focus is driven by conservation concerns where the health of pteropod populations is threatened by the high susceptibility of their shells to dissolution in low aragonite saturation states associated with OA and how coupling of these stressors may push pteropods past the limits of physiological plasticity. In this manipulation experiment, we describe changes in the transcriptome of the Antarctic pteropod, Limacina helicina antarctica, to these combined stressors. The conditions used in the laboratory treatments met or exceeded those projected for the Southern Ocean by the year 2100. We made two general observations regarding the outcome of the data: (1) Temperature was more influential than pH in terms of changing patterns of gene expression, and (2) these Antarctic pteropods appeared to have a significant degree of transcriptomic plasticity to respond to acute abiotic stress in the laboratory. In general, differential gene expression was observed amongst the treatments; here, for example, transcripts associated with maintaining protein structure and cell proliferation were up-regulated. To disentangle the effects of OA and OW, we used a weighted gene co-expression network analysis to explore patterns of change in the transcriptome. This approach identified gene networks associated with OW that were enriched for transcripts proposed to be involved in increasing membrane fluidity at warmer temperatures. Together these data provide evidence that L.h.antarctica has a limited capacity to acclimate to the combined conditions of OA and OW used in this study. This reduced scope of acclimation argues for continued study of how adaptation to polar aquatic environments may limit the plasticity of present-day populations in responding to future environmental change.

Differential Gene Expression of CCKA and CCKB Receptors in the Rat Brain

1993 ◽  
Vol 4 (2) ◽  
pp. 143-154 ◽  
Author(s):  
Toyohiko Honda ◽  
Etsuko Wada ◽  
James F. Battey ◽  
Stephen A. Wank
Keyword(s):  
Gene Expression ◽  
Rat Brain ◽  
Differential Gene Expression ◽  
Differential Gene ◽  
Cckb Receptors

scholarly journals Physical Activity Induces Nucleus Accumbens Genes Expression Changes Preventing Chronic Pain Susceptibility Promoted by High-Fat Diet and Sedentary Behavior in Mice

2019 ◽  
Author(s):  
Arthur de Freitas Brandão ◽  
Ivan José Magayewski Bonet ◽  
Marco Pagliusi ◽  
Gabriel Gerardini Zanetti ◽  
Nam Pho ◽  
...  
Keyword(s):  
Gene Expression ◽  
Physical Activity ◽  
Chronic Pain ◽  
Gene Ontology ◽  
Sedentary Behavior ◽  
Differential Gene Expression ◽  
High Fat Diet ◽  
Biological Processes ◽  
High Fat ◽  
Differential Gene

AbstractHigh-fat diet (HFD)-induced obesity was reported to increase pain behavior independent of obesity status in rats, whereas weight loss interventions such as voluntary physical activity (PA) for adults with overweight or obesity was reported to promote pain reduction in humans with chronic pain (CP). However, is unknown whether an HFD and sedentary (SED) behavior is underlying to CP susceptibility and whether voluntary PA can prevent it. Moreover, differential gene expression in the nucleus accumbens (NAc) is considered to play a crucial role in CP susceptibility. The present study used an adapted model of the inflammatory prostaglandin E2 (PGE)-induced persistent hyperalgesia (PH-ST) protocol for mice, an HFD, and a voluntary PA paradigm to test these hypotheses. In addition, we performed a transcriptome in the NAc and a gene ontology enrichment tools to investigate the differential gene expression and identify the biological processes associated with CP susceptibility tested here. Our results demonstrated that HFD and sedentary behavior promoted CP susceptibility, which in turn was prevented by voluntary PA, even when the animals were fed an HFD. Transcriptome in the NAc found 2,204 differential expression genes related CP susceptibility promoted by HFD and sedentary behavior and prevented by voluntary PA. The gene ontology enrichment revealed 41 biological processes implicated in CP susceptibility. Analyzing collectively those biological processes, our results suggested that genes related to metabolic and mitochondria stress were up-regulated in the CP susceptibility group, whereas genes related to neuroplasticity and axonogenesis were up-regulated in the CP prevented group. These findings provide pieces of evidence that an HFD and sedentary behavior promoted gene expression changes in the NAc related to neurodegeneration and those changes were also underlying to CP susceptibility. Additionally, our findings confirmed other findings supporting the crucial role of voluntary PA to prevent CP susceptibility and add novel insights of differential gene expression in the NAc related to neuroplasticity.

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