scholarly journals Paradoxical Regulation of Allogeneic Bone Marrow Engraftment and Immune Privilege by Mesenchymal Cells and Adenosine

2020 ◽  
Author(s):  
Miwako Kakiuchi ◽  
Yuichi Hirata ◽  
Simon C. Robson ◽  
Joji Fujisaki
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Cells ◽  
Allogeneic Bone Marrow ◽  
Immune Privilege ◽  
Allogeneic Bone ◽  
Bone Marrow Engraftment

A novel application of cyclosporine A in nonmyeloablative pretransplant host conditioning for allogeneic BMT

Blood ◽  
2000 ◽  
Vol 96 (3) ◽  
pp. 1166-1172 ◽  
Author(s):  
Boris Nikolic ◽  
Guiling Zhao ◽  
Kirsten Swenson ◽  
Megan Sykes
Keyword(s):  
Bone Marrow ◽  
Cyclosporine A ◽  
Bone Marrow Cells ◽  
Corticosteroid Treatment ◽  
Marrow Transplant ◽  
Allogeneic Bone Marrow ◽  
Whole Body ◽  
Allogeneic Bone ◽  
Central Tolerance ◽  
Bone Marrow Engraftment

The treatment of mice with anti-CD4 and anti-CD8 monoclonal antibodies (mAbs) on day −5, plus 3 Gy whole body irradiation (WBI) and 7 Gy thymic irradiation (TI) on day 0, allows fully major-histocompatibility-complex–mismatched allogeneic bone marrow engraftment and the induction of immunologic tolerance. TI is required in this model to overcome alloreactivity and possibly to make “space” in the recipient thymus so that lasting central tolerance can be achieved. In addition to suppressing mature T cells in the periphery, Cyclosporine A (CYA) and glucocorticoids have a powerful influence on the thymus. In this study, we evaluated whether the administration of CYA to recipient mice for 12 days prior to bone marrow transplant (BMT), of glucocorticosteroids on the day of BMT, or a combination of both, could create space and overcome alloresistance in the thymus by specifically depleting immature and mature thymocytes prior to BMT. High levels of multilineage donor hematopoietic repopulation and specific transplantation tolerance were achieved in mice treated from days −15 to −3 with CYA (20 mg/kg/d subcutaneously), anti-CD4/CD8 mAbs on day −5, followed by 3 Gy WBI and 15 × 106 allogeneic bone marrow cells on day 0. Vβ analysis suggested a central deletional tolerance mechanism. The same treatment without CYA pretreatment allowed only transient chimerism, without tolerance. Corticosteroid treatment abolished the engraftment-promoting and tolerance-inducing effects of CYA. These results demonstrate a novel pretransplantation-only application of CYA, which facilitates allogeneic marrow engraftment with minimal conditioning, by creating thymic space and/or overcoming intrathymic alloresistance.

scholarly journals Marrow Engraftment by Allogeneic Leukocytes in Lethally Irradiated Dogs

Blood ◽  
1967 ◽  
Vol 30 (6) ◽  
pp. 805-811 ◽  
Author(s):  
R. STORB ◽  
R. B. EPSTEIN ◽  
H. RAGDE ◽  
J. BRYANT ◽  
E. D. THOMAS
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Blood Count ◽  
White Blood Cells ◽  
Allogeneic Bone Marrow ◽  
Allogeneic Bone ◽  
Single Donor ◽  
Cytogenetic Studies ◽  
Bone Marrow Engraftment

Abstract Infusion of white blood cells separated from peripheral blood produced allogeneic bone marrow engraftment in lethally irradiated dogs. Approximately 100 x 109 leukocytes obtained from a single donor over an 8-day period were adequate to establish marrow repopulation. Marrow engraftment was indicated by rising blood count, marrow histology, and, in one instance, cytogenetic studies. Marrow grafts were associated with a severe secondary syndrome. Survival was prolonged with methotrexate.

scholarly journals Fine mapping of the Bmgr5 quantitative trait locus for allogeneic bone marrow engraftment in mice

2013 ◽  
Vol 65 (8) ◽  
pp. 585-596 ◽  
Author(s):  
Yuanyuan Wang ◽  
Xinjian Chen ◽  
Schickwann Tsai ◽  
Alun Thomas ◽  
Judith A. Shizuru ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Quantitative Trait Locus ◽  
Fine Mapping ◽  
Quantitative Trait ◽  
Allogeneic Bone Marrow ◽  
Allogeneic Bone ◽  
Trait Locus ◽  
Bone Marrow Engraftment

scholarly journals Thiotepa improves allogeneic bone marrow engraftment without enhancing stem cell depletion in irradiated mice

1998 ◽  
Vol 21 (4) ◽  
pp. 327-330 ◽  
Author(s):  
JD Down ◽  
GR Westerhof ◽  
A Boudewijn ◽  
R Setroikromo ◽  
RE Ploemacher
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Allogeneic Bone Marrow ◽  
Cell Depletion ◽  
Allogeneic Bone ◽  
Bone Marrow Engraftment

scholarly journals Amifostine prior to lethal irradiation prevents allogeneic bone marrow engraftment in mice

2008 ◽  
Vol 41 (11) ◽  
pp. 927-934 ◽  
Author(s):  
J S Thompson ◽  
R Asmis ◽  
Y Chu ◽  
J Glass ◽  
B Nelson ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Allogeneic Bone Marrow ◽  
Allogeneic Bone ◽  
Bone Marrow Engraftment ◽  
Lethal Irradiation

scholarly journals Mouse host unlicensed NK cells promote donor allogeneic bone marrow engraftment

Blood ◽  
2016 ◽  
Vol 127 (9) ◽  
pp. 1202-1205 ◽  
Author(s):  
Maite Alvarez ◽  
Kai Sun ◽  
William J. Murphy
Keyword(s):  
Bone Marrow ◽  
Nk Cells ◽  
Allogeneic Bone Marrow ◽  
Allogeneic Bone ◽  
Gm Csf ◽  
Key Points ◽  
Bone Marrow Engraftment

Key Points Unlicensed NK cells release GM-CSF upon allogeneic MHCI recognition, which promotes donor allogeneic BMC engraftment.

scholarly journals Keratinocyte Growth Factor Improves Allogeneic Bone Marrow Engraftment through a CD4+Foxp3+Regulatory T Cell-Dependent Mechanism

2009 ◽  
Vol 182 (12) ◽  
pp. 7364-7369 ◽  
Author(s):  
Marieke Bruinsma ◽  
Peter L. van Soest ◽  
Pieter J. M. Leenen ◽  
Bob Löwenberg ◽  
Jan J. Cornelissen ◽  
...  
Keyword(s):  
Bone Marrow ◽  
T Cell ◽  
Growth Factor ◽  
Regulatory T Cell ◽  
Keratinocyte Growth Factor ◽  
Allogeneic Bone Marrow ◽  
Allogeneic Bone ◽  
Bone Marrow Engraftment ◽  
Dependent Mechanism
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