scholarly journals The Therapeutic Effect of Immune-Modifying Microparticles in an Acute Graft-Versus-Host Disease Model

2019 ◽  
Vol 25 (3) ◽  
pp. S298-S300
Author(s):  
John P. Galvin ◽  
Sara A. Beddow ◽  
Stephen Miller
Keyword(s):  
Therapeutic Effect ◽  
Graft Versus Host Disease ◽  
Disease Model ◽  
Host Disease ◽  
Graft Versus Host ◽  
Acute Graft

Increased Intestinal Permeability and Disturbance of Tight-Junctions in an Acute Graft Versus Host Disease Model in Mice

2011 ◽  
Vol 140 (5) ◽  
pp. S-504
Author(s):  
Rainer Noth ◽  
Julia Lange-Grumfeld ◽  
Jochen Hampe ◽  
Stefan Schreiber ◽  
Alexander Arlt
Keyword(s):  
Tight Junctions ◽  
Intestinal Permeability ◽  
Graft Versus Host Disease ◽  
Disease Model ◽  
Host Disease ◽  
Graft Versus Host ◽  
Acute Graft

scholarly journals Systematic comparison of hUC-MSCs at various passages reveals the variations of signatures and therapeutic effect on acute graft-versus-host disease

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Qinjun Zhao ◽  
Leisheng Zhang ◽  
Yimeng Wei ◽  
Hao Yu ◽  
Linglin Zou ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Therapeutic Effect ◽  
Graft Versus Host Disease ◽  
Cytokine Secretion ◽  
Differentiation Potential ◽  
Host Disease ◽  
Graft Versus Host ◽  
Acute Graft

Abstract Background Mesenchymal stem cells are heterogenous populations with hematopoietic supporting and immunomodulating capacities. Enormous studies have focused on their preclinical or clinical therapeutic effects, yet the systematic study of continuous in vitro passages on signatures and functions of UC-MSCs at both the cellular and molecular levels is still lacking. Methods In this study, to systematically evaluate the biological properties of MSCs at various passages, we analyzed biomarker expression, cell proliferation and apoptosis, chromosome karyotype, and tri-lineage differentiation potential. Subsequently, we took advantage of whole-exome sequencing to compare the somatic hypermutation of hUC-MSCs at P3, P6, and P15 including SNV and INDEL mutations. In addition, to explore the safety of the abovementioned hUC-MSCs, we performed metabolic pathway enrichment analysis and in vivo transplantation analysis. Furthermore, we cocultured the abovementioned hUC-MSCs with UCB-CD34+ HSCs to evaluate their hematopoietic supporting capacity in vitro. Finally, we transplanted the cells into acute graft-versus-host disease (aGVHD) mice to further evaluate their therapeutic effect in vivo. Results The hUC-MSCs at P3, P6, and P15 showed similar morphology, biomarker expression, and cytokine secretion. hUC-MSCs at P15 had advantages on adipogenic differentiation and some cytokine secretion such as IL-6 and VEGF, with disadvantages on cell proliferation, apoptosis, and osteogenic and chondrogenic differentiation potential. Based on the SNP data of 334,378 exons and bioinformatic analyses, we found the somatic point mutations could be divided into 96 subsets and formed 30 kinds of signatures but did not show correlation with risk of tumorigenesis, which was confirmed by the in vivo transplantation experiments. However, hUC-MSCs at P15 showed impaired hematologic supporting effect in vitro and declined therapeutic effect on aGVHD in vivo. Conclusions In this study, we systematically evaluated the biological and genetic properties of hUC-MSCs at various passages. Our findings have provided new references for safety and effectiveness assessments, which will provide overwhelming evidence for the safety of hUC-MSCs after continuous in vitro passages both at the cellular and molecular levels for the first time. Taken together, our studies could help understand the controversial effects of disease treatment and benefit the clinical research of UC-MSCs.

Generation of allo-antigen-specific induced Treg stabilized by vitamin C treatment and its application for prevention of acute graft versus host disease model

2017 ◽  
Vol 29 (10) ◽  
pp. 457-469 ◽  
Author(s):  
Hidenori Kasahara ◽  
Taisuke Kondo ◽  
Hiroko Nakatsukasa ◽  
Shunsuke Chikuma ◽  
Minako Ito ◽  
...  
Keyword(s):  
Vitamin C ◽  
Graft Versus Host Disease ◽  
Disease Model ◽  
Host Disease ◽  
Graft Versus Host ◽  
Acute Graft

Therapeutic Effect of Novel Anti-Human Fas Antibody HFE7A on Graft-versus-Host Disease Model

2001 ◽  
Vol 99 (3) ◽  
pp. 340-346 ◽  
Author(s):  
Harumi Kuwahara ◽  
Yoshio Tani ◽  
Yukie Ogawa ◽  
Yasuhiro Takaichi ◽  
Akio Shiraishi ◽  
...  
Keyword(s):  
Therapeutic Effect ◽  
Graft Versus Host Disease ◽  
Disease Model ◽  
Host Disease ◽  
Graft Versus Host

Oral Glutamine Supplementation Improves Intestinal Permeability Dysfunction in a Murine Acute Graft Versus Host Disease Model

2011 ◽  
Vol 140 (5) ◽  
pp. S-167
Author(s):  
Rainer Noth ◽  
Robert Haesler ◽  
Julia Lange-Grumfeld ◽  
Jochen Hampe ◽  
Stefan Schreiber ◽  
...  
Keyword(s):  
Intestinal Permeability ◽  
Graft Versus Host Disease ◽  
Disease Model ◽  
Glutamine Supplementation ◽  
Host Disease ◽  
Graft Versus Host ◽  
Acute Graft
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