scholarly journals Co-Administration of Mgta-145 and Plerixafor Rapidly Mobilizes High Numbers of Hematopoietic Stem Cells and Graft-Versus-Host Disease Inhibiting Monocytic Cells in Non-Human Primates

2019 ◽  
Vol 25 (3) ◽  
pp. S291-S292 ◽  
Author(s):  
Kevin A. Goncalves ◽  
Patrick C. Falahee ◽  
Sharon L. Hyzy ◽  
Shuping Li ◽  
Anthony E. Boitano ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Graft Versus Host Disease ◽  
Monocytic Cells ◽  
Hematopoietic Stem ◽  
Host Disease ◽  
Graft Versus Host

scholarly journals Ex Vivo Gene Therapy: Graft-versus-host Disease (GVHD) in NSG™ (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) Mice Transplanted with CD34+ Human Hematopoietic Stem Cells

2019 ◽  
Vol 47 (5) ◽  
pp. 656-660 ◽  
Author(s):  
Sundeep Chandra ◽  
Patrizia Cristofori ◽  
Carlos Fonck ◽  
Charles A. O’Neill
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Bone Marrow ◽  
Hematopoietic Stem Cells ◽  
Graft Versus Host Disease ◽  
Ex Vivo ◽  
Hematopoietic Stem ◽  
Host Disease ◽  
Graft Versus Host ◽  
Human Cd34

A therapeutic option for monogenic disorders is gene therapy with ex vivo-transduced autologous hematopoietic stem cells (HSCs). Safety or efficacy studies of ex vivo-modified HSCs are conducted in humanized mouse models after ablation of the murine bone marrow and transfer of human CD34+ HSCs. Engrafted human CD34+ cells migrate to bone marrow and differentiate into various human hematopoietic lineages. A 12-week study was conducted in NSG™ mice to evaluate engraftment, differentiation, and safety of human CD34+ cells that were transduced ( ex vivo) with a proprietary lentiviral vector encoding a human gene (BMRN-1) or a mock (green fluorescent protein) vector. Several mice intravenously injected with naive CD34+ cells or transduced CD34+ cells had variable lymphohistiocytic inflammatory cell infiltrates and microgranulomas in the liver and lungs consistent with graft-versus-host disease (GVHD). Spleen, bone marrow, stomach, reproductive tract, but not the skin had similar inflammatory changes. Ex vivo viral transduction of CD34+ cells did not impact engraftment or predispose to xenogeneic GVHD.

State of gata2 gene in hematopoietic stem cells of recipients with graft versus host disease induced by cryopreserved allogeneic material

2015 ◽  
Vol 25 (1) ◽  
pp. 67-75
Author(s):  
Anatoliy N. Goltsev ◽  
◽  
Tatyana G. Dubrava ◽  
Yuliya A. Gaevskaya ◽  
Elena D. Lutsenko ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Graft Versus Host Disease ◽  
Hematopoietic Stem ◽  
Host Disease ◽  
Graft Versus Host
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