scholarly journals Long-Term Immune Reconstitution of Naive and Memory T Cell Pools after Haploidentical Hematopoietic Stem Cell Transplantation

2013 ◽  
Vol 19 (5) ◽  
pp. 703-712 ◽  
Author(s):  
Rita I. Azevedo ◽  
Maria V.D. Soares ◽  
Adriana S. Albuquerque ◽  
Rita Tendeiro ◽  
Rui S. Soares ◽  
...  
Keyword(s):  
Stem Cell ◽  
T Cell ◽  
Hematopoietic Stem Cell Transplantation ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Hematopoietic Stem Cell ◽  
Immune Reconstitution ◽  
Hematopoietic Stem ◽  
Memory T Cell

Long-term T-cell reconstitution after hematopoietic stem-cell transplantation in primary T-cell–immunodeficient patients is associated with myeloid chimerism and possibly the primary disease phenotype

Blood ◽  
2007 ◽  
Vol 109 (10) ◽  
pp. 4575-4581 ◽  
Author(s):  
Marina Cavazzana-Calvo ◽  
Frédérique Carlier ◽  
Françoise Le Deist ◽  
Estelle Morillon ◽  
Pierre Taupin ◽  
...  
Keyword(s):  
Stem Cell ◽  
T Cell ◽  
Hematopoietic Stem Cell Transplantation ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Hematopoietic Stem Cell ◽  
Cell Receptor ◽  
Hematopoietic Stem ◽  
T Cell Reconstitution

Abstract We studied T-cell reconstitution in 31 primary T-cell–immunodeficient patients who had undergone hematopoietic stem-cell transplantation (HSCT) over 10 years previously. In 19 patients, there was no evidence of myeloid chimerism because little or no myeloablation had been performed. Given this context, we sought factors associated with good long-term T-cell reconstitution. We found that all patients having undergone full myeloablation had donor myeloid cells and persistent thymopoiesis, as evidenced by the presence of naive T cells carrying T-cell receptor excision circles (TRECs). In 9 patients with host myeloid chimerism, sustained thymic output was also observed and appeared to be associated with γc deficiency. It is therefore possible that the complete absence of thymic progenitors characterizing this condition created a more favorable environment for thymic seeding by a population of early progenitor cells with the potential for self-renewal, thus enabling long-term (> 10 years) T-cell production.

scholarly journals Immune reconstitution following hematopoietic stem cell transplantation, monitoring parameters and new methods of its assessment

2002 ◽  
Vol 55 (7-8) ◽  
pp. 293-298 ◽  
Author(s):  
Svetlana Vojvodic
Keyword(s):  
Stem Cell ◽  
T Cell ◽  
Hematopoietic Stem Cell Transplantation ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Hematopoietic Stem Cell ◽  
Immune Reconstitution ◽  
Hematopoietic Stem ◽  
Thymic Function ◽  
New Methods

Introduction Despite engraftment and function of graft after hematopoietic stem cell transplantation severe immune suppression is a characteristic of early posttransplant period. Primary parameters contributing to posttransplant immunoincompetence include: lack of sustained transfer of donor antigen specific immunity, recapitulation of immunological ontogeny effect of graft-versus-host disease (GvHD) and its therapy and reduction of the recipient's thymic function. Normal immune reconstitution following allogeneic hematopoietic stem cell transplantation (HSCT) Immune reconstitution following allogeneic HSCT depends on the age of recipient initial pathology, degree of HLA and minor histocompatibility antigen mismatches, T-cell depletion and use of anti-T-cell antibodies for conditioning and prevention of GvHD, posttransplant complications such as acute and chronic GvHD, relapse of disease and infectious status of the donor and recipient prior to transplantation. Tests for studying immune reconstitution following allogeneic transplantation most often used are cutaneous tests of delayed hypersensitivity, phenotyping of blood mononuclear cells and investigation of their functions, production of antibodies (IgG, IgM and IgA) and cytokines such as IL-2, TGF-a, TNF-a or IL-10, study of TCR, mixed leukocyte reaction toward allogeneic cells CD4/CD8 ratio and others. New tools for assessment of immune reconstitution after HSCT New methods and techniques for monitoring immune reconstitution are as follows: ELISPOT assay quantifies secretion of cytokines by T lymphocytes, analysis of the T-cell receptor (chain diversity during immune reconstitution by IMMUNOSCOPE/SPECTRATYPING method, and TREC technology that detects recent thymic emigrants in peripheral blood. Conclusion Usage of above-mentioned contemporary techniques makes it possible to assess and monitor the dynamics of immune reconstitution especially reconstitution of T-cell diversity, thymic function and antigen-specific T-cell response following HSCT.

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