scholarly journals The role of peyer’s patches and mesenteric lymph nodes in acute GVHD: a study guided by bioluminescence imaging in vivo

2004 ◽  
Vol 10 ◽  
pp. 5
Author(s):  
A. Beilhack ◽  
S. Schulz ◽  
C.B. Wieland ◽  
G.F. Beilhack ◽  
J. Baker ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Acute Gvhd ◽  
Bioluminescence Imaging ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph

scholarly journals Eosinophilia in transgenic mice expressing interleukin 5.

1990 ◽  
Vol 172 (5) ◽  
pp. 1425-1431 ◽  
Author(s):  
L A Dent ◽  
M Strath ◽  
A L Mellor ◽  
C J Sanderson
Keyword(s):  
Bone Marrow ◽  
Transgenic Mice ◽  
Lymph Nodes ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Mesenteric Lymph Nodes ◽  
Gene Encoding ◽  
Interleukin 5

Experiments in vitro suggest that although interleukin 5 (IL-5) stimulates the late stages of eosinophil differentiation, other cytokines are required for the generation of eosinophil progenitor cells. In this study transgenic mice constitutively expressing the IL-5 gene were established using a genomic fragment of the IL-5 gene coupled to the dominant control region from the gene encoding human CD2. Four independent eosinophilic transgenic lines have thus far been established, two of which with 8 and 49 transgene copies, are described in detail. These mice appeared macroscopically normal apart from splenomegaly. Eosinophils were at least 65- and 265-fold higher in blood from transgenics, relative to normal littermates, and approximately two- or sevenfold more numerous relative to blood from mice infected with the helminth Mesocestoides corti. Much more modest increases in blood neutrophil, lymphocyte, and monocyte numbers were noted in transgenics, relative to normal littermates (less than threefold). Thus IL-5 in vivo is relatively specific for the eosinophil lineage. Large numbers of eosinophils were present in spleen, bone marrow, and peritoneal exudate, and were highest in the line with the greatest transgene copy number. Eosinophilia was also noted in histological sections of transgenic lungs, Peyer's patches, mesenteric lymph nodes, and gut lamina propria but not in other tissues examined. IL-5 was detected in the sera of transgenics at levels comparable to those seen in sera from parasite-infected animals. IL-3 and granulocyte/macrophage colony-stimulating factor (GM-CSF) were not found. IL-5 mRNA was detected in transgenic thymus, Peyer's patches, and superficial lymph nodes, but not in heart, liver, brain, or skeletal muscle or in any tissues from nontransgenics. Bone marrow from transgenic mice was rich in IL-5-dependent eosinophil precursors. These data indicate that induction of the IL-5 gene is sufficient for production of eosinophilia, and that IL-5 can induce the full pathway of eosinophil differentiation. IL-5 may therefore not be restricted in action to the later stages of eosinophil differentiation, as suggested by earlier in vitro studies.

scholarly journals Commensal microbiota drive proliferation of conventional and Foxp3+Regulatory CD4+T cells in mesenteric lymph nodes and Peyer's patches

2013 ◽  
Vol 3 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Sascha Cording ◽  
Diana Fleissner ◽  
Markus M. Heimesaat ◽  
Stefan Bereswill ◽  
Christoph Loddenkemper ◽  
...  
Keyword(s):  
T Cells ◽  
Lymph Nodes ◽  
Cd4 T Cells ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Mesenteric Lymph Nodes ◽  
Commensal Microbiota ◽  
Mesenteric Lymph

scholarly journals Oral Immunization with ATP-Dependent Protease-Deficient Mutants Protects Mice against Subsequent Oral Challenge with Virulent Salmonella enterica Serovar Typhimurium

2003 ◽  
Vol 71 (1) ◽  
pp. 30-39 ◽  
Author(s):  
Hidenori Matsui ◽  
Masato Suzuki ◽  
Yasunori Isshiki ◽  
Chie Kodama ◽  
Masahiro Eguchi ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Salmonella Enterica ◽  
Peyer's Patches ◽  
Oral Challenge ◽  
Secretory Iga ◽  
Peyer’S Patches ◽  
Lon Protease ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Serovar Typhimurium

ABSTRACT We evaluated the efficacy of mutants with a deletion of the stress response protease gene as candidates for live oral vaccine strains against Salmonella infection through infection studies with mice by using a Salmonella enterica serovar Typhimurium mutant with a disruption of the ClpXP or Lon protease. In vitro, the ClpXP protease regulates flagellum synthesis and the ClpXP-deficient mutant strain exhibits hyperflagellated bacterial cells (T. Tomoyasu et al., J. Bacteriol. 184:645-653, 2002). On the other hand, the Lon protease negatively regulates the efficacy of invading epithelial cells and the expression of invasion genes (A. Takaya et al., J. Bacteriol. 184:224-232, 2002). When 5-week-old BALB/c mice were orally administered 5 × 108 CFU of the ClpXP- or Lon-deficient strain, bacteria were detected with 103 to 104 CFU in the spleen, mesenteric lymph nodes, Peyer's patches, and cecum 1 week after inoculation and the bacteria then decreased gradually in each tissue. Significant increases of lipopolysaccharide-specific immunoglobulin G (IgG) and secretory IgA were detected at week 4 and maintained until at least week 12 after inoculation in serum and bile, respectively. Immunization with the ClpXP- or Lon-deficient strain protected mice against oral challenge with the serovar Typhimurium virulent strain. Both the challenged virulent and immunized avirulent salmonellae were completely cleared from the spleen, mesenteric lymph nodes, Peyer's patches, and even cecum 5 days after the challenge. These data indicate that Salmonella with a disruption of the ATP-dependent protease ClpXP or Lon can be useful in developing a live vaccine strain.

scholarly journals Diabetes-prone BioBreeding rats do not have a normal immune response when weaned to a diet containing fermentable fibre

2005 ◽  
Vol 93 (5) ◽  
pp. 645-653 ◽  
Author(s):  
RoseMarie Stillie ◽  
Rhonda C. Bell ◽  
Catherine J. Field
Keyword(s):  
Lymph Nodes ◽  
Stimulation Index ◽  
Control Diet ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Intestinal Length ◽  
Small Intestinal ◽  
Normal Immune Response

Diet is known to modulate the development of diabetes in diabetes-prone BioBreeding (BBdp) rats. The objective of the present study was to determine the effect of fermentable fibre (FF) on immune function in BBdp and diabetes-resistant BioBreeding (BBdr) rats after weaning. Weanling BBdp (thirty-six to thirty-eight per diet) and BBdr rats (thirty to thirty-two per diet) were fed a nutritionally complete, semi-purified, casein-based diet containing either cellulose (control diet, 8 % w/w) or FF (3·2 % cellulose+4·8 % w/w inulin). At 35 d, the small intestine was excised and lymphocytes isolated from spleen, mesenteric lymph nodes and Peyer's patches. Feeding FF to both BBdr and BBdp rats affected the production of anti-inflammatory cytokines (P=0·02). In BBdr rats, feeding FF compared with cellulose resulted in an increased small intestinal length (P=0·0031), higher proliferative (stimulation) index from both splenocytes (P=0·001) and mesenteric lymph nodes (P=0·04), and an increased proportion of CD8+ T-cells in the Peyer's patches (P=0·003). We did not observe an effect of diet on the number of IgA-bearing cells in the jejunum from BBdr rats. Feeding FF to BBdp rats did not affect the same parameters. BBdp rats had both a higher proportion of B-cells in the Peyer's patches (P=0·01) and a higher number of IgA+ cells in the jejunum (P=0·0036) when fed a diet containing FF, a response not observed in BBdr rats. We demonstrate that several aspects of the BBdp immune system respond differently than that of BBdr rats when challenged at weaning with FF.

W1784 Neither the Peyer's Patches, Mesenteric Lymph Nodes nor the Spleen are Required for Induction of Chronic Colitis

2010 ◽  
Vol 138 (5) ◽  
pp. S-739
Author(s):  
Koichi Takebayashi ◽  
Iurii Koboziev ◽  
Laura Gray ◽  
Fridrik Karlsson ◽  
Dmitry V. Ostanin ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Chronic Colitis ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph

Development of apoptosis and changes in lymphocyte subsetsin thymus, mesenteric lymph nodes and Peyer's patches of mice orally inoculated with T-2 toxin

2001 ◽  
Vol 53 (4) ◽  
pp. 309-315 ◽  
Author(s):  
Takayuki Nagata ◽  
Hodaka Suzuki ◽  
Noriaki Ishigami ◽  
Junko Shinozuka ◽  
Kouji Uetsuka ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph

Modification of lymphocytes of peyer's patches - mesenteric lymph nodes - spleen by egg-white lysozyme

1995 ◽  
Vol 31 ◽  
pp. 363
Author(s):  
S. Pacor ◽  
G. Sava ◽  
A. Bergamo ◽  
E. Giacomello ◽  
R. Gagliardi ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Egg White ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Mesenteric Lymph Nodes ◽  
Egg White Lysozyme ◽  
Mesenteric Lymph
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