Involvement of glia in central sensitization in trigeminal subnucleus caudalis (medullary dorsal horn)

2007 ◽  
Vol 21 (5) ◽  
pp. 634-641 ◽  
Author(s):  
Y.F. Xie ◽  
S. Zhang ◽  
C.Y. Chiang ◽  
J.W. Hu ◽  
J.O. Dostrovsky ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
Central Sensitization ◽  
Medullary Dorsal Horn ◽  
Trigeminal Subnucleus Caudalis

Endogenous ATP Involvement in Mustard-Oil-Induced Central Sensitization in Trigeminal Subnucleus Caudalis (Medullary Dorsal Horn)

2005 ◽  
Vol 94 (3) ◽  
pp. 1751-1760 ◽  
Author(s):  
C. Y. Chiang ◽  
S. Zhang ◽  
Y. F. Xie ◽  
J. W. Hu ◽  
J. O. Dostrovsky ◽  
...  
Keyword(s):  
Receptor Antagonist ◽  
Dorsal Horn ◽  
Central Sensitization ◽  
P2x Receptor ◽  
Field Size ◽  
Mustard Oil ◽  
Tooth Pulp ◽  
Nociceptive Neurons ◽  
Medullary Dorsal Horn ◽  
Trigeminal Subnucleus Caudalis

Central sensitization represents a sustained hypersensitive state of dorsal horn nociceptive neurons that can be evoked by peripheral inflammation or injury to nerves and tissues. It reflects neuroplastic changes such as increases in neuronal spontaneous activity, receptive field size, and responses to suprathreshold stimuli and a decrease in activation threshold. We recently demonstrated that purinergic receptor mechanisms in trigeminal subnucleus caudalis (Vc; medullary dorsal horn) are also involved in the initiation and maintenance of central sensitization in brain stem nociceptive neurons of trigeminal subnucleus oralis. The aim of the present study was to investigate whether endogenous ATP is involved in the development of central sensitization in Vc itself. The experiments were carried out on urethan/α-chloralose anesthetized and immobilized rats. Single neurons were recorded and identified as nociceptive-specific (NS) in the deep laminae of Vc. During continuous saline superfusion (0.6 ml/h it) over the caudal medulla, Vc neuronal central sensitization was readily induced by mustard oil application to the tooth pulp. However, this mustard-oil-induced central sensitization could be completely blocked by continuous intrathecal superfusion of the wide-spectrum P2X receptor antagonist pyridoxal-phosphate-6-azophenyl-2, 4-disulphonic acid tetra-sodium (33–100 μM) and by apyrase (an ectonucleotidase enzyme, 30 units/ml). Superfusion of the selective P2X1, P2X3 and P2X2/3 receptor antagonist 2′,3′- O-(2,4,6-trinitrophenyl) adenosine 5′-triphosphate (6–638 μM) partially blocked the Vc central sensitization. The two P2X receptor antagonists did not significantly affect the baseline nociceptive properties of the Vc neurons. These findings implicate endogenous ATP as an important mediator contributing to the development of central sensitization in nociceptive neurons of the deep laminae of the dorsal horn.

scholarly journals Astroglial Glutamate Glutamine Shuttle Is Involved in Central Sensitization of Nociceptive Neurons in Rat Medullary Dorsal Horn

2007 ◽  
Vol 27 (34) ◽  
pp. 9068-9076 ◽  
Author(s):  
C.-Y. Chiang ◽  
J. Wang ◽  
Y.-F. Xie ◽  
S. Zhang ◽  
J. W. Hu ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
Central Sensitization ◽  
Nociceptive Neurons ◽  
Medullary Dorsal Horn

scholarly journals Glutamine uptake contributes to central sensitization in the medullary dorsal horn

Neuroreport ◽  
2008 ◽  
Vol 19 (11) ◽  
pp. 1151-1154 ◽  
Author(s):  
Chen Yu Chiang ◽  
Zhaohui Li ◽  
Jonathan O. Dostrovsky ◽  
James W. Hu ◽  
Barry J. Sessle
Keyword(s):  
Dorsal Horn ◽  
Central Sensitization ◽  
Medullary Dorsal Horn ◽  
Glutamine Uptake

scholarly journals Central α-adrenoceptors contribute to mustard oil-induced central sensitization in the rat medullary dorsal horn

Neuroscience ◽  
2013 ◽  
Vol 236 ◽  
pp. 244-252 ◽  
Author(s):  
H. Wang ◽  
Y.F. Xie ◽  
C.Y. Chiang ◽  
J.O. Dostrovsky ◽  
B.J. Sessle
Keyword(s):  
Dorsal Horn ◽  
Central Sensitization ◽  
Mustard Oil ◽  
Medullary Dorsal Horn
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