Intact spore MALDI-TOF mass spectrometry and proteomic analysis of Puccinia pathogenic fungi

2016 ◽  
Vol 1864 (9) ◽  
pp. 1093-1103 ◽  
Author(s):  
Jana Beinhauer ◽  
Martin Raus ◽  
Alena Hanzalová ◽  
Pavel Horčička ◽  
Marek Šebela
Keyword(s):  
Mass Spectrometry ◽  
Proteomic Analysis ◽  
Pathogenic Fungi ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

scholarly journals Proteomic analysis of canine oral tumor tissues using MALDI-TOF mass spectrometry and in-gel digestion coupled with mass spectrometry (GeLC MS/MS) approaches

PLoS ONE ◽  
2018 ◽  
Vol 13 (7) ◽  
pp. e0200619 ◽  
Author(s):  
Sirinun Pisamai ◽  
Sittiruk Roytrakul ◽  
Narumon Phaonakrop ◽  
Janthima Jaresitthikunchai ◽  
Gunnaporn Suriyaphol
Keyword(s):  
Mass Spectrometry ◽  
Proteomic Analysis ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Oral Tumor ◽  
Tumor Tissues

scholarly journals MALDI-TOF mass spectrometry applied to identifying species of insect-pathogenic fungi from the Metarhizium anisopliae complex

Mycologia ◽  
2014 ◽  
Vol 106 (4) ◽  
pp. 865-878 ◽  
Author(s):  
Rogério Biaggioni Lopes ◽  
Marcos Faria ◽  
Daniela Aguiar Souza ◽  
Carlos Bloch ◽  
Luciano P. Silva ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Metarhizium Anisopliae ◽  
Pathogenic Fungi ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Insect Pathogenic Fungi

scholarly journals Entacapone Treatment Modulates Hippocampal Proteins Related to Synaptic Vehicle Trafficking

Cells ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 2712
Author(s):  
Dae Young Yoo ◽  
Hyo Young Jung ◽  
Woosuk Kim ◽  
Kyu Ri Hahn ◽  
Hyun Jung Kwon ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Proteomic Analysis ◽  
Neurological Diseases ◽  
Reversible Inhibitor ◽  
Synapsin I ◽  
Enzymatic Reactions ◽  
Methyl Transferase ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Dynamin I

Entacapone, a reversible inhibitor of catechol-O-methyl transferase, is used for patients in Parkinson’s disease because it increases the bioavailability and effectiveness of levodopa. In the present study, we observed that entacapone increases novel object recognition and neuroblasts in the hippocampus. In the present study, two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry were performed to compare the abundance profiles of proteins expressed in the hippocampus after entacapone treatment in mice. Results of 2-DE, MALDI-TOF mass spectrometry, and subsequent proteomic analysis revealed an altered protein expression profile in the hippocampus after entacapone treatment. Based on proteomic analysis, 556 spots were paired during the image analysis of 2-DE gels and 76 proteins were significantly changed more than two-fold among identified proteins. Proteomic analysis indicated that treatment with entacapone induced expressional changes in proteins involved in synaptic transmission, cellular processes, cellular signaling, the regulation of cytoskeletal structure, energy metabolism, and various subcellular enzymatic reactions. In particular, entacapone significantly increased proteins related to synaptic trafficking and plasticity, such as dynamin 1, synapsin I, and Munc18-1. Immunohistochemical staining showed the localization of the proteins, and western blot confirmed the significant increases in dynamin I (203.5% of control) in the hippocampus as well as synapsin I (254.0% of control) and Munc18-1 (167.1% of control) in the synaptic vesicle fraction of hippocampus after entacapone treatment. These results suggest that entacapone can enhance hippocampal synaptic trafficking and plasticity against various neurological diseases related to hippocampal dysfunction.

A proteomic analysis of Pseudevernia furfuracea after exposure to Cr+6 by MALDI-TOF mass spectrometry

3 Biotech ◽  
2021 ◽  
Vol 11 (10) ◽  
Author(s):  
Sinem Özenoğlu-Aydınoğlu ◽  
Hatice Yıldızhan ◽  
Demet Cansaran-Duman
Keyword(s):  
Mass Spectrometry ◽  
Proteomic Analysis ◽  
Maldi Tof Mass Spectrometry ◽  
Pseudevernia Furfuracea ◽  
Maldi Tof
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