Alpha-synuclein and familial variants affect the chain order and the thermotropic phase behavior of anionic lipid vesicles

2016 ◽  
Vol 1864 (9) ◽  
pp. 1206-1214 ◽  
Author(s):  
Manuela Pantusa ◽  
Brian Vad ◽  
Ove Lillelund ◽  
Lars Kjær ◽  
Daniel Otzen ◽  
...  
Keyword(s):  
Phase Behavior ◽  
Lipid Vesicles ◽  
Alpha Synuclein ◽  
Anionic Lipid ◽  
Chain Order

scholarly journals Conformational dynamics and phase behavior of lipid vesicles in a precisely controlled extensional flow

Soft Matter ◽  
2020 ◽  
Vol 16 (2) ◽  
pp. 337-347 ◽  
Author(s):  
Dinesh Kumar ◽  
Channing M. Richter ◽  
Charles M. Schroeder
Keyword(s):  
Phase Behavior ◽  
Phase Diagrams ◽  
Direct Observation ◽  
Conformational Dynamics ◽  
Extensional Flow ◽  
Lipid Vesicles ◽  
Precise Characterization ◽  
Flow Phase

Direct observation of vesicle conformational dynamics in extensional flow and precise characterization of flow phase diagrams using a Stokes trap.

Dependence of Bicellar System Phase Behavior and Dynamics on Anionic Lipid Concentration

Langmuir ◽  
2013 ◽  
Vol 29 (11) ◽  
pp. 3688-3699 ◽  
Author(s):  
Lauren MacEachern ◽  
Alexander Sylvester ◽  
Alanna Flynn ◽  
Ashkan Rahmani ◽  
Michael R. Morrow
Keyword(s):  
Phase Behavior ◽  
Lipid Concentration ◽  
Anionic Lipid ◽  
System Phase

Interaction of α-and β-Oligoarginine-Acids and Amides with Anionic Lipid Vesicles:  A Mechanistic and Thermodynamic Study†

Biochemistry ◽  
2006 ◽  
Vol 45 (18) ◽  
pp. 5817-5829 ◽  
Author(s):  
Thomas Hitz ◽  
Rico Iten ◽  
James Gardiner ◽  
Kenji Namoto ◽  
Peter Walde ◽  
...  
Keyword(s):  
Lipid Vesicles ◽  
Thermodynamic Study ◽  
Anionic Lipid

scholarly journals Effects of Temperature on the Phase Behavior and Permeability of Thylakoid Lipid Vesicles

1992 ◽  
Vol 99 (3) ◽  
pp. 912-918 ◽  
Author(s):  
Murray S. Webb ◽  
Daniel V. Lynch ◽  
Beverley R. Green
Keyword(s):  
Phase Behavior ◽  
Lipid Vesicles ◽  
Effects Of Temperature

Effect of the aspartic acid D2 on the affinity of Polybia-MP1 to anionic lipid vesicles

2014 ◽  
Author(s):  
Natália Bueno Leite ◽  
Dayane dos Santos Alvares ◽  
Bibiana Monson de Souza ◽  
Mário Sérgio Palma ◽  
João Ruggiero Neto
Keyword(s):  
Aspartic Acid ◽  
Lipid Vesicles ◽  
Anionic Lipid

Myotoxin a-Phospholipid Interactions, an Attempt by Intrinsic Fluorescence to Define a Possible Mode of Action for the Toxin on Membranes

1987 ◽  
Vol 41 (8) ◽  
pp. 1410-1417 ◽  
Author(s):  
Jean Dufourco ◽  
Francoise Dousseau ◽  
Jean-FrançOis Faucon ◽  
Anthony T. Tu
Keyword(s):  
Blue Shift ◽  
Lipid Vesicles ◽  
Intrinsic Fluorescence ◽  
Net Charge ◽  
Crotalus Viridis ◽  
Chain Order ◽  
Tryptophan Residues ◽  
Lipid Chain ◽  
Myotoxin A ◽  
First Time

Intrinsic fluorescence of myotoxin a from Crotalus viridis viridis has been proved to be due to tryptophan residues, and this knowledge allows one to detect, for the first time, an interaction of the toxin with phospholipids. The formation of lipid-protein species is followed by a blue shift of about 8 nm and a quenching of up to 50% of the emission of tryptophans. These changes could result from the positioning of Trp 32,34 residues at the lipid interface and/or a local conformational change of the toxin. In the μM range, complexes are formed only with charged lipids; charge complementarity of two partners is needed regardless of the physical state of the lipids. The binding is electrostatic, and the affinity of myotoxin a decreases in parallel with the net charge of the phospholipid interface and/or that of the toxin. Similarly, calcium-ion addition, pH changes, and an increase in ionic strength can dissociate the complexes. Their stability increases in the following sequence: phosphatidylinositol < phosphatidylserine < phosphatidylglycerol < phosphatidic acid < cardiolipin. It is proposed that the toxin lies at the interface, without penetrating the membrane. On binding, the toxin does not severely change the structure of lipid vesicles nor their permeability; however, significant changes in the lipid chain order are detected with synthetic lipids, both in their gel and fluid phases. The possible consequences of the existence of myotoxin a-charged phospholipid complexes are discussed and are compared to the behavior of other related basic toxins of snake venom acting on membrane.

scholarly journals Modulatory Effects of Acidic pH and Membrane Potential on the Adsorption of pH-Sensitive Peptides to Anionic Lipid Membrane

Membranes ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 307
Author(s):  
Dayane dos Santos Alvares ◽  
Ingrid Bernardes Santana Martins ◽  
Taisa Giordano Viegas ◽  
Mario Sergio Palma ◽  
Alexandre Suman de Araujo ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Adsorption Isotherms ◽  
Lipid Membrane ◽  
Lipid Vesicles ◽  
Md Simulations ◽  
Acidic Ph ◽  
Solution Ph ◽  
Dynamic Simulations ◽  
Anionic Lipid ◽  
Peptide Adsorption

Anionic lipid membrane electrostatic potential and solution pH can influence cationic peptide adsorption to these bilayers, especially those containing simultaneously acid and basic residues. Here, we investigate the effects of the pH solution on MP1 (IDWKKLLDAAKQIL-NH2) adsorption to anionic (7POPC:3POPG) lipid vesicles in comparison to its analog H-MP1, with histidines substituting lysines. We used the association of adsorption isotherms and constant pH molecular dynamic simulations (CpHMD) to explore the effects of membrane potential and pH on peptides’ adsorption on this lipid membrane. We analyzed the fluorescence and zeta potential adsorption isotherms using the Gouy–Chapman theory. In CpHMD simulations for the peptides in solution and adsorbed on the lipid bilayer, we used the conformations obtained by conventional MD simulations at a μs timescale. Non-equilibrium Monte Carlo simulations provided the protonation states of acidic and basic residues. CpHMD showed average pKa shifts of two to three units, resulting in a higher net charge for the analog than for MP1, strongly modulating the peptide adsorption. The fractions of the protonation of acidic and basic residues and the peptides’ net charges obtained from the analysis of the adsorption isotherms were in reasonable agreement with those from CpHMD. MP1 adsorption was almost insensitive to solution pH. H-MP1 was much more sensitive to partitioning, at acidic pH, with an affinity ten times higher than in neutral ones.

scholarly journals Influence of Lipid Composition on Pediocin PA-1 Binding to Phospholipid Vesicles

1998 ◽  
Vol 64 (9) ◽  
pp. 3530-3532 ◽  
Author(s):  
Yuhuan Chen ◽  
Richard D. Ludescher ◽  
Thomas J. Montville
Keyword(s):  
Fatty Acid ◽  
Electrostatic Interaction ◽  
Lipid Content ◽  
Interaction Model ◽  
Lipid Vesicles ◽  
Phospholipid Vesicles ◽  
Binding Parameters ◽  
Lipid Concentration ◽  
Anionic Lipid ◽  
Lipid Interactions

ABSTRACT Pediocin PA-1 bound to anionic lipid vesicles with saturated or unsaturated fatty acid chains in a lipid concentration-dependent fashion. Little change in binding parameters was observed for zwitterionic lipid vesicles. Decreasing the anionic lipid content of the vesicles gave a higher relative dissociation constant for the peptide-lipid interactions and further supports the electrostatic interaction model of binding.

An investigation into the critical tension of electroporation in anionic lipid vesicles

2020 ◽  
Author(s):  
Mohammad Abu Sayem Karal ◽  
Md. Kabir Ahamed ◽  
Urbi Shyamolima Orchi ◽  
Md. Towhiduzzaman ◽  
Marzuk Ahmed ◽  
...  
Keyword(s):  
Lipid Vesicles ◽  
Anionic Lipid ◽  
Critical Tension
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