Caudal-related homeodomain proteins CDX1/2 bind to DNA replication-related element binding factor

2011 ◽  
Vol 1814 (12) ◽  
pp. 1891-1899 ◽  
Author(s):  
So Young Park ◽  
Mi Suk Jeong ◽  
Mi-Ae Yoo ◽  
Se Bok Jang
Keyword(s):  
Dna Replication ◽  
Homeodomain Proteins ◽  
Binding Factor ◽  
Related Element

scholarly journals Redox regulation of DNA binding activity of DREF (DNA replication-related element binding factor) in Drosophila

2004 ◽  
Vol 378 (3) ◽  
pp. 833-838 ◽  
Author(s):  
Tae-Yeong CHOI ◽  
S. Young PARK ◽  
Ho-Sung KANG ◽  
Jae-Hun CHEONG ◽  
Han-Do KIM ◽  
...  
Keyword(s):  
Dna Replication ◽  
Dna Binding ◽  
Redox State ◽  
Redox Regulation ◽  
Binding Activity ◽  
Dna Binding Activity ◽  
Cell Extracts ◽  
Binding Factor ◽  
Related Element ◽  
Intracellular Redox

DREF [DRE (DNA replication-related element) binding factor] is an 80 kDa polypeptide homodimer which plays an important role in regulating cell proliferation-related genes. Both DNA binding and dimer formation activities are associated with residues 16–115 of the N-terminal region. However, the mechanisms by which DREF dimerization and DNA binding are regulated remain unknown. Here, we report that the DNA binding activity of DREF is regulated by a redox mechanism, and that the cysteine residues are involved in this regulation. Electrophoretic mobility shift analysis using Drosophila Kc cell extracts or recombinant DREF proteins indicated that the DNA binding domain is sufficient for redox regulation. Site-directed mutagenesis and transient transfection assays showed that Cys59 and/or Cys62 are critical both for DNA binding and for redox regulation, whereas Cys91 is dispensable. In addition, experiments using Kc cells indicated that the DNA binding activity and function of DREF are affected by the intracellular redox state. These findings give insight into the exact nature of DREF function in the regulation of target genes by the intracellular redox state.

scholarly journals Transcriptional regulation of the Drosophila-raf proto-oncogene by the DNA replication-related element (DRE)/DRE-binding factor (DREF) system

1997 ◽  
Vol 25 (4) ◽  
pp. 794-799 ◽  
Author(s):  
J.-R. Ryu ◽  
T.-Y. Choi ◽  
E.-J. Kwon ◽  
W.-H. Lee ◽  
Y. Nishida ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Dna Replication ◽  
Binding Factor ◽  
Related Element

scholarly journals The DNA Replication-related Element (DRE)/DRE-binding Factor System Is a Transcriptional Regulator of theDrosophila E2FGene

1998 ◽  
Vol 273 (40) ◽  
pp. 26042-26051 ◽  
Author(s):  
Tomoyuki Sawado ◽  
Fumiko Hirose ◽  
Yasuhiko Takahashi ◽  
Takayo Sasaki ◽  
Tomoyuki Shinomiya ◽  
...  
Keyword(s):  
Dna Replication ◽  
Transcriptional Regulator ◽  
Binding Factor ◽  
Factor System ◽  
Related Element

The DNA replication-related element (DRE)-DRE-binding factor (DREF) system may be involved in the expression of the Drosophila melanogaster TBP gene

FEBS Letters ◽  
2000 ◽  
Vol 483 (1) ◽  
pp. 71-77 ◽  
Author(s):  
Tae-Yeong Choi ◽  
Nam-Young Cho ◽  
Younsang Oh ◽  
Mi-Ae Yoo ◽  
Akio Matsukage ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Dna Replication ◽  
Binding Factor ◽  
Tbp Gene ◽  
Related Element

scholarly journals Human DNA Replication-related Element Binding Factor (hDREF) Self-association via hATC Domain Is Necessary for Its Nuclear Accumulation and DNA Binding

2007 ◽  
Vol 282 (10) ◽  
pp. 7563-7575 ◽  
Author(s):  
Daisuke Yamashita ◽  
Hirofumi Komori ◽  
Yoshiki Higuchi ◽  
Tomohiro Yamaguchi ◽  
Takashi Osumi ◽  
...  
Keyword(s):  
Dna Replication ◽  
Dna Binding ◽  
Nuclear Accumulation ◽  
Human Dna ◽  
Binding Factor ◽  
Self Association ◽  
Related Element

scholarly journals Dynamic changes in the genomic localization of DNA replication-related element binding factor during the cell cycle

Cell Cycle ◽  
2013 ◽  
Vol 12 (10) ◽  
pp. 1605-1615 ◽  
Author(s):  
B Gurudatta ◽  
Jingping Yang ◽  
Kevin Van Bortle ◽  
Paul Donlin-Asp ◽  
Victor Corces
Keyword(s):  
Cell Cycle ◽  
Dna Replication ◽  
Dynamic Changes ◽  
Binding Factor ◽  
Related Element
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