Crystallization, X-ray diffraction analysis and phasing of carboxylesterase PA3859 from Pseudomonas aeruginosa

2005 ◽  
Vol 1752 (2) ◽  
pp. 197-201 ◽  
Author(s):  
Alessandro Pesaresi ◽  
Doriano Lamba
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Diffraction Analysis ◽  
X Ray Diffraction ◽  
X Ray

scholarly journals Evidence that cysteine-166 is the active-site nucleophile of Pseudomonas aeruginosa amidase: crystallization and preliminary X-ray diffraction analysis of the enzyme

1999 ◽  
Vol 340 (3) ◽  
pp. 711-714 ◽  
Author(s):  
Sebastien FARNAUD ◽  
Renée TATA ◽  
Maninder K. SOHI ◽  
Tommy WAN ◽  
Paul R. BROWN ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Diffraction Analysis ◽  
Active Site ◽  
Quaternary Structure ◽  
Cross Reactivity ◽  
Polyclonal Antiserum ◽  
Wild Type ◽  
X Ray Diffraction ◽  
Wild Type Enzyme ◽  
X Ray

Wild-type and site-specific mutants C166S and C166A (Cys-166 → Ser and Cys-166 → Ala respectively) of the amidase (acylamide amidohydrolase, EC 3.5.1.4) from Pseudomonas aeruginosa were expressed in Escherichia coli by using the vector pKK223-3. Both mutant proteins were catalytically inactive but showed complete cross-reactivity with polyclonal antiserum raised against the wild-type enzyme, as well as CD spectra identical with that of the wild-type enzyme, which were indicative of correct folding. Cys-166 is therefore implicated as the active-site nucleophile. Titration of free thiol groups with 5,5ʹ-dithiobis-(2-nitrobenzoic acid) indicated that Cys-166 is not a rapidly reacting residue. Crystals of both wild-type and C166S amidase grew with identical, rhombohedral morphology; X-ray diffraction analysis established the unit cell dimensions (a = b = c = 84 Å; α = β = γ = 75 °) and space group (R3 or R32). These results imply a quaternary structure of six subunits, with most probably 32 symmetry; the existence of a hexameric structure was supported by molecular mass determinations based on gel filtration and electrophoretic mobility.

A New Germacranolide and a New Ceramide from Salvia nubicola (Lamiaceae)

2007 ◽  
Vol 62 (10) ◽  
pp. 1333-1338 ◽  
Author(s):  
Muhammad S. Ali ◽  
Syed A. Ibrahima ◽  
Shakeel Ahmed ◽  
Emil Lobkovsky
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Growth Inhibition ◽  
Single Crystal ◽  
Diffraction Analysis ◽  
Lemna Minor ◽  
Spectroscopic Techniques ◽  
X Ray Diffraction ◽  
Strong Growth ◽  
X Ray ◽  
Soluble Part

The methanol-soluble part of Salvia nubicola has yielded a new germacranolide and a new ceramide named nubtrienolide and nubenamide, respectively. Structures of both the isolated constituents were elucidated with the aid of spectroscopic techniques; the nubtrienolide structure was further confirmed via single crystal X-ray diffraction analysis. Among both the metabolites, nubtrienolide showed strong growth inhibition against Pseudomonas aeruginosa while during phytotoxicity testing, the same compound promoted the growth of Lemna minor L. instead to inhibit it.

scholarly journals Evidence that cysteine-166 is the active-site nucleophile of Pseudomonas aeruginosa amidase: crystallization and preliminary X-ray diffraction analysis of the enzyme

1999 ◽  
Vol 340 (3) ◽  
pp. 711 ◽  
Author(s):  
Sebastien FARNAUD ◽  
Renée TATA ◽  
Maninder K. SOHI ◽  
Tommy WAN ◽  
Paul R. BROWN ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Diffraction Analysis ◽  
Active Site ◽  
X Ray Diffraction ◽  
X Ray

Ten-Vertex Polyhedral Monocarbaborane Chemistry. The Novel High-Yield Formation of the Ten-Vertex closo Compound [6-(PMe2Ph)-closo-1-CB9H9] from the Thermolysis of [8,8-(PMe2Ph)2-nido-8,7-PtCB9H11]

1993 ◽  
Vol 58 (12) ◽  
pp. 2924-2935 ◽  
Author(s):  
Jane H. Jones ◽  
Bohumil Štíbr ◽  
John D. Kennedy ◽  
Mark Thornton-Pett
Keyword(s):  
Nmr Spectroscopy ◽  
Diffraction Analysis ◽  
High Yield ◽  
Monoclinic Space Group ◽  
The Novel ◽  
X Ray Diffraction ◽  
Metal Centre ◽  
X Ray ◽  
Yield Formation ◽  
Boiling Toluene

Thermolysis of [8,8-(PMe2Ph)2-nido-8,7-PtCB9H11] in boiling toluene solution results in an elimination of the platinum centre and cluster closure to give the ten-vertex closo species [6-(PMe2Ph)-closo-1-CB9H9] in 85% yield as a colourles air stable solid. The product is characterized by NMR spectroscopy and single-crystal X-ray diffraction analysis. Crystals (from hexane-dichloromethane) are monoclinic, space group P21/c, with a = 903.20(9), b = 1 481.86(11), c = 2 320.0(2) pm, β = 97.860(7)° and Z = 8, and the structure has been refined to R(Rw) = 0.045(0.051) for 3 281 observed reflections with Fo > 2.0σ(Fo). The clean high-yield elimination of a metal centre from a polyhedral metallaborane or metallaheteroborane species is very rare.

Bis(μ-carboxylato)dienerhodium(I) Complexes - Synthesis, Characterization and Catalytic Activity

2008 ◽  
Vol 73 (8-9) ◽  
pp. 1205-1221 ◽  
Author(s):  
Jiří Zedník ◽  
Jan Sedláček ◽  
Jan Svoboda ◽  
Jiří Vohlídal ◽  
Dmitrij Bondarev ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Diffraction Analysis ◽  
Spectroscopic Methods ◽  
X Ray Diffraction ◽  
X Ray

Dinuclear rhodium(I) η2:η2-cycloocta-1,5-diene (series a) and η2:η2-norborna-2,5-diene (series b) complexes with μ-RCOO- ligands, where R is linear C21H43 (complexes 1a, 1b), CH2CMe3 (2a, 2b), 1-adamantyl (3a, 3b) and benzyl (4a, 4b), have been prepared and characterized by spectroscopic methods. Structures of complexes 2b, 3a and 4a were determined by X-ray diffraction analysis. Complexes prepared show low to moderate catalytic activity in polymerization of phenylacetylene in THF giving high-cis-transoid polymers, but they show only oligomerization activity in dichloromethane.

scholarly journals Crystals of the trp repressor-operator complex suitable for X-ray diffraction analysis.

1987 ◽  
Vol 262 (10) ◽  
pp. 4917-4921 ◽  
Author(s):  
A. Joachimiak ◽  
R.Q. Marmorstein ◽  
R.W. Schevitz ◽  
W. Mandecki ◽  
J.L. Fox ◽  
...  
Keyword(s):  
Diffraction Analysis ◽  
X Ray Diffraction ◽  
Trp Repressor ◽  
X Ray
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