scholarly journals Probing the effect of membrane contents on transmembrane protein-protein interaction using solution NMR and computer simulations

2018 ◽  
Vol 1860 (12) ◽  
pp. 2486-2498 ◽  
Author(s):  
P.E. Bragin ◽  
A.S. Kuznetsov ◽  
O.V. Bocharova ◽  
P.E. Volynsky ◽  
A.S. Arseniev ◽  
...  
Keyword(s):  
Computer Simulations ◽  
Protein Interaction ◽  
Transmembrane Protein ◽  
Solution Nmr ◽  
Protein Protein Interaction

An efficient transient assays system using Agrobacterium-mediated transformation of onion (Allium cepa) epidermal cells

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Yu-Miao Zhang ◽  
Jun Wang ◽  
Tao Wu
Keyword(s):  
Subcellular Localization ◽  
Protein Interaction ◽  
Protein Interactions ◽  
Epidermal Cells ◽  
Cyclin Dependent Kinase ◽  
Protein Subcellular Localization ◽  
Protein Protein Interactions ◽  
Efficient System ◽  
Protein Protein Interaction ◽  
Onion Epidermal Cells

In this study, the Agrobacterium infection medium, infection duration, detergent, and cell density were optimized. The sorghum-based infection medium (SbIM), 10-20 min infection time, addition of 0.01% Silwet L-77, and Agrobacterium optical density at 600 nm (OD600), improved the competence of onion epidermal cells to support Agrobacterium infection at >90% efficiency. Cyclin-dependent kinase D-2 (CDKD-2) and cytochrome c-type biogenesis protein (CYCH), protein-protein interactions were localized. The optimized procedure is a quick and efficient system for examining protein subcellular localization and protein-protein interaction.

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