scholarly journals The biological response of cells to nanosecond pulsed electric fields is dependent on plasma membrane cholesterol

2016 ◽  
Vol 1858 (11) ◽  
pp. 2636-2646 ◽  
Author(s):  
Jody C. Cantu ◽  
Melissa Tarango ◽  
Hope T. Beier ◽  
Bennett L. Ibey
Keyword(s):  
Plasma Membrane ◽  
Electric Fields ◽  
Biological Response ◽  
Pulsed Electric Fields ◽  
Membrane Cholesterol ◽  
Nanosecond Pulsed Electric Fields ◽  
Plasma Membrane Cholesterol

Plasma Membrane Charging of Jurkat Cells by Nanosecond Pulsed Electric Fields

2007 ◽  
Author(s):  
Juergen F. Kolb ◽  
Jody A. White ◽  
Uwe Pliquett ◽  
Richard Nuccitelli ◽  
Karl H. Schoenbach ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Jurkat Cells ◽  
Nanosecond Pulsed Electric Fields

Plasma membrane charging of Jurkat cells by nanosecond pulsed electric fields

2011 ◽  
Vol 40 (8) ◽  
pp. 947-957 ◽  
Author(s):  
Jody A. White ◽  
Uwe Pliquett ◽  
Peter F. Blackmore ◽  
Ravindra P. Joshi ◽  
Karl H. Schoenbach ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Jurkat Cells ◽  
Nanosecond Pulsed Electric Fields

scholarly journals Nonlinear dispersive cell model for microdosimetry of nanosecond pulsed electric fields

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Fei Guo ◽  
Lin Zhang ◽  
Xin Liu
Keyword(s):  
Plasma Membrane ◽  
Electric Fields ◽  
Time Domain ◽  
Time Course ◽  
Cell Model ◽  
Pulsed Electric Fields ◽  
Polarization Vector ◽  
Temporal And Spatial Distribution ◽  
Nanosecond Pulsed Electric Fields ◽  
Debye Equation

Abstract For applications based on nanosecond pulsed electric fields (nsPEFs), the underlying transmembrane potential (TMP) distribution on the plasma membrane is influenced by electroporation (EP) of the plasma membrane and dielectric dispersion (DP) of all cell compartments which is important for predicting the bioelectric effects. In this study, the temporal and spatial distribution of TMP on the plasma membrane induced by nsPEFs of various pulse durations (3 ns, 5 ns unipolar, 5 ns bipolar, and 10 ns) is investigated with the inclusion of both DP and EP. Based on the double-shelled dielectric spherical cell model, the Debye equation describing DP is transformed into the time-domain form with the introduction of polarization vector, and then we obtain the time course of TMP by solving the combination of Laplace equation and time-domain Debye equation. Next, the asymptotic version of the Smoluchowski equation is included to characterize the EP of plasma membrane in order to observe more profound electroporation effects with larger pore density and electroporated areas in consideration of both DP and EP. Through the simulation, it is clearer to understand the relationship between the applied nsPEFs and the induced bioelectric effects.

scholarly journals Monoclonal antibody detection of plasma membrane cholesterol microdomains responsive to cholesterol trafficking

2001 ◽  
Vol 42 (9) ◽  
pp. 1492-1500 ◽  
Author(s):  
Howard S. Kruth ◽  
Ina Ifrim ◽  
Janet Chang ◽  
Lia Addadi ◽  
Daniele Perl-Treves ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Plasma Membrane ◽  
Antibody Detection ◽  
Membrane Cholesterol ◽  
Cholesterol Trafficking ◽  
Plasma Membrane Cholesterol

Cell culturing in a three-dimensional matrix affects the localization and properties of plasma membrane cholesterol

2009 ◽  
Vol 33 (10) ◽  
pp. 1079-1086 ◽  
Author(s):  
Nadezhda Stefanova ◽  
Galya Staneva ◽  
Diana Petkova ◽  
Teodora Lupanova ◽  
Roumen Pankov ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Three Dimensional ◽  
Membrane Cholesterol ◽  
Dimensional Matrix ◽  
Cell Culturing ◽  
Plasma Membrane Cholesterol

Differential effects of nanosecond pulsed electric fields on cells representing progressive ovarian cancer

2021 ◽  
Vol 142 ◽  
pp. 107942
Author(s):  
Hongmei Liu ◽  
Yajun Zhao ◽  
Chenguo Yao ◽  
Eva M. Schmelz ◽  
Rafael V. Davalos
Keyword(s):  
Ovarian Cancer ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Differential Effects ◽  
Nanosecond Pulsed Electric Fields

scholarly journals Time-Lapse, Live-Cell Imaging of Potassium Efflux in Cell Exposed to Nanosecond Pulsed Electric Fields

2021 ◽  
Vol 120 (3) ◽  
pp. 223a
Author(s):  
Flavia Mazzarda ◽  
Esin B. Sozer ◽  
Julia L. Pittaluga ◽  
Claudia Muratori ◽  
P. Thomas Vernier
Keyword(s):  
Electric Fields ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Pulsed Electric Fields ◽  
Time Lapse ◽  
Live Cell ◽  
Potassium Efflux ◽  
Nanosecond Pulsed Electric Fields
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