scholarly journals A patch clamp study on the electro-permeabilization of higher plant cells: Supra-physiological voltages induce a high-conductance, K+ selective state of the plasma membrane

2011 ◽  
Vol 1808 (6) ◽  
pp. 1728-1736 ◽  
Author(s):  
Lars H. Wegner ◽  
Bianca Flickinger ◽  
Christian Eing ◽  
Thomas Berghöfer ◽  
Petra Hohenberger ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Patch Clamp ◽  
Plant Cells ◽  
Higher Plant ◽  
Clamp Study ◽  
High Conductance

Plasma membrane ion channel regulation during abscisic acid-induced closing of stomata

1992 ◽  
Vol 338 (1283) ◽  
pp. 83-89 ◽  
Keyword(s):  
Plasma Membrane ◽  
Abscisic Acid ◽  
Ion Channels ◽  
Patch Clamp ◽  
Integrated Control ◽  
Anion Channel ◽  
Guard Cells ◽  
Anion Channels ◽  
Higher Plant ◽  
Ion Channel Regulation

The plant growth regulator abscisic acid triggers closing of stomata in the leaf epidermis in response to water stress. Recent tracer flux studies, patch-clamp studies, fluorometric Ca 2+ measurements and microelectrode experiments have provided insight into primary transduction mechanisms by which abscisic acid causes stomatal closing. Data show that abscisic acid activates non-selective Ca 2+ permeable ion channels in the plasma membrane of guard cells. The resulting elevation in the free Ca 2+ concentration in the cytosol of guard cells, and the resulting membrane depolarization as well as other unidentified Ca 2+ independent mechanisms are suggested to contribute to activation of voltage- and second messenger-dependent anion channels and outward rectifying K + channels. Recent data suggest the involvement of two types of anion channels in the regulation of stomatal movements, which provide highly distinct mechanisms for anion efflux and depolarization. A novely characterized ‘S-type’ anion channel is likely to provide a key mechanism for long-term depolarization and sustained anion efflux during closing of stomata. Patch-clamp studies have revealed the presence of a network of K + , anion and non-selective Ca 2+ -permeable channels in the plasma membrane of a higher plant cell. The integrated control of these guard cell ion channels by abscisic acid can provide control over K + and anion efflux required for stomatal closing.

scholarly journals Visualization of particle complexes in the plasma membrane of Micrasterias denticulata associated with the formation of cellulose fibrils in primary and secondary cell walls.

1980 ◽  
Vol 84 (2) ◽  
pp. 327-339 ◽  
Author(s):  
T H Giddings ◽  
D L Brower ◽  
L A Staehelin
Keyword(s):  
Plasma Membrane ◽  
Green Alga ◽  
Plasma Membranes ◽  
Plant Cells ◽  
Higher Plant ◽  
Cellulose Fibrils ◽  
Micrasterias Denticulata ◽  
Wall Growth ◽  
Fern Protonemata ◽  
Center Distance

Highly ordered arrays of intramembrane particles are observed in freeze-fractured plasma membranes of the green alga Micrasterias denticulata during the synthesis of the secondary cell wall. The observable architecture of the complex consists primarily of a precise hexagonal array of from 3 to 175 rosettes, consisting of 6 particles each, which fracture with the P-face. The complexes are observed at the ends of impressions of cellulose fibrils. The distance between rows of rosettes is equal to the center-to-center distance between parallel cellulose fibrils of the secondary wall. Correlation of the structure of the complex with the pattern of deposition indicates that the size of a given fibril is proportional to the number of rosettes engaged in its formation. Vesicles containing hexagonal arrays of rosettes are found in the cytoplasm and can be observed in the process of fusing with the plasma membrane, suggesting that the complexes are first assembled in the cytoplasm and then incorporated into the plasma membrane, where they become active in fibril formation. Single rosettes appear to be responsible for the synthesis of microfibrils during primary wall growth. Similar rosettes have now been detected in a green alga, in fern protonemata, and in higher plant cells. This structure, therefore, probably represents a significant component of the cellulose synthesizing mechanism in a large variety of plant cells.

Patch-clamp studies on higher plant cells: a perspective

1987 ◽  
Vol 12 ◽  
pp. 49-52 ◽  
Author(s):  
R. Hedrichh ◽  
J.I. Schroeder ◽  
J.M. Fernandez
Keyword(s):  
Patch Clamp ◽  
Plant Cells ◽  
Higher Plant

Enrichment of vitronectin- and fibronectin-like proteins in NaCI-adapted plant cells and evidence for their involvement in plasma membrane-cell wall adhesion

1993 ◽  
Vol 3 (5) ◽  
pp. 637-646 ◽  
Author(s):  
Jian-Kang Zhu ◽  
Jun Shi ◽  
Utpal Singh ◽  
Sarah E. Wyatt ◽  
Ray A. Bressan ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Plant Cells ◽  
Membrane Cell
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