Nonsense-mediated mRNA decay — Mechanisms of substrate mRNA recognition and degradation in mammalian cells

2013 ◽  
Vol 1829 (6-7) ◽  
pp. 612-623 ◽  
Author(s):  
Christoph Schweingruber ◽  
Simone C. Rufener ◽  
David Zünd ◽  
Akio Yamashita ◽  
Oliver Mühlemann
Keyword(s):  
Mammalian Cells ◽  
Mrna Decay ◽  
Nonsense Mediated Mrna Decay

scholarly journals SMG-1 Is a Phosphatidylinositol Kinase-Related Protein Kinase Required for Nonsense-Mediated mRNA Decay in Caenorhabditis elegans

2004 ◽  
Vol 24 (17) ◽  
pp. 7483-7490 ◽  
Author(s):  
Andrew Grimson ◽  
Sean O'Connor ◽  
Carrie Loushin Newman ◽  
Philip Anderson
Keyword(s):  
Protein Kinase ◽  
Mammalian Cells ◽  
Mrna Decay ◽  
Null Alleles ◽  
Dependent Manner ◽  
Messenger Rnas ◽  
Phosphatidylinositol Kinase ◽  
Nonsense Mediated Mrna Decay

ABSTRACT Eukaryotic messenger RNAs containing premature stop codons are selectively and rapidly degraded, a phenomenon termed nonsense-mediated mRNA decay (NMD). Previous studies with both Caenohabditis elegans and mammalian cells indicate that SMG-2/human UPF1, a central regulator of NMD, is phosphorylated in an SMG-1-dependent manner. We report here that smg-1, which is required for NMD in C. elegans, encodes a protein kinase of the phosphatidylinositol kinase superfamily of protein kinases. We identify null alleles of smg-1 and demonstrate that SMG-1 kinase activity is required in vivo for NMD and in vitro for SMG-2 phosphorylation. SMG-1 and SMG-2 coimmunoprecipitate from crude extracts, and this interaction is maintained in smg-3 and smg-4 mutants, both of which are required for SMG-2 phosphorylation in vivo and in vitro. SMG-2 is located diffusely through the cytoplasm, and its location is unaltered in mutants that disrupt the cycle of SMG-2 phosphorylation. We discuss the role of SMG-2 phosphorylation in NMD.

scholarly journals Gene expression networks: competing mRNA decay pathways in mammalian cells

2009 ◽  
Vol 37 (6) ◽  
pp. 1287-1292 ◽  
Author(s):  
Lynne E. Maquat ◽  
Chenguang Gong
Keyword(s):  
Gene Expression ◽  
Present Article ◽  
Mammalian Cells ◽  
Mrna Decay ◽  
C2c12 Myoblasts ◽  
Nonsense Mediated Mrna Decay ◽  
Multinucleated Myotubes

Nonsense-mediated mRNA decay and Staufen1-mediated mRNA decay are mechanistically related pathways that serve distinct purposes. In the present article, we give an overview of each pathway. We describe how a factor that is common to both pathways results in their competition. We also explain how competition between the two pathways contributes to the differentiation of C2C12 myoblasts to multinucleated myotubes.

Insulin Signaling Augments eIF4E-Dependent Nonsense-Mediated mRNA Decay in Mammalian Cells

2016 ◽  
Vol 1859 (7) ◽  
pp. 896-905 ◽  
Author(s):  
Jungyun Park ◽  
Seyoung Ahn ◽  
Aravinth K. Jayabalan ◽  
Takbum Ohn ◽  
Hyun Chul Koh ◽  
...  
Keyword(s):  
Insulin Signaling ◽  
Mammalian Cells ◽  
Mrna Decay ◽  
Nonsense Mediated Mrna Decay

Nonsense-mediated mRNA decay

2008 ◽  
Vol 36 (3) ◽  
pp. 514-516 ◽  
Author(s):  
Jikai Wen ◽  
Saverio Brogna
Keyword(s):  
Mammalian Cells ◽  
Mrna Decay ◽  
Translation Termination ◽  
Mrna Splicing ◽  
Current Understanding ◽  
Coupled Processes ◽  
Recent Developments ◽  
Nonsense Mediated Mrna Decay ◽  
Premature Translation Termination

Translation and mRNA decay are coupled processes; the link is most obvious in the case of NMD (nonsense-mediated mRNA decay). NMD is a mechanism that drastically reduces the level of mRNA harbouring PTCs (premature translation termination codons). The defining event in NMD is premature translation termination and the key question is: what distinguishes premature from normal translation termination? Surprisingly, in mammalian cells, PTC recognition is linked to pre-mRNA splicing. Here, we review the current understanding in view of recent developments.

scholarly journals A Haploid Genetic Screening Method for Proteins Influencing Mammalian Nonsense-Mediated mRNA Decay Activity

2018 ◽  
Author(s):  
Maximilian W. Popp ◽  
Lynne E. Maquat
Keyword(s):  
Genetic Screening ◽  
Mammalian Cells ◽  
Mrna Decay ◽  
Fluorescent Proteins ◽  
Screening Method ◽  
Transcript Abundance ◽  
Exon Junction Complex ◽  
Exon Junction ◽  
Nonsense Mediated Mrna Decay

AbstractDespite a long appreciation for the role of nonsense-mediated mRNA decay (NMD) in the destruction of faulty, disease-causing mRNAs, as well as its role in the maintenance of normal, endogenous transcript abundance, systematic unbiased methods for uncovering modifiers of NMD activity in mammalian cells remain scant. Here we present and validate a haploid genetic screening method for identifying proteins and processes that stimulate NMD activity involving a 3′-untranslated region exon-junction complex. This reporterbased screening method can be adapted for interrogating other pathways whose output can be measured by the intracellular production of fluorescent proteins.

CBP80 promotes interaction of Upf1 with Upf2 during nonsense-mediated mRNA decay in mammalian cells

2005 ◽  
Vol 12 (10) ◽  
pp. 893-901 ◽  
Author(s):  
Nao Hosoda ◽  
Yoon Ki Kim ◽  
Fabrice Lejeune ◽  
Lynne E Maquat
Keyword(s):  
Mammalian Cells ◽  
Mrna Decay ◽  
Nonsense Mediated Mrna Decay

scholarly journals The Dharma of Nonsense-Mediated mRNA Decay in Mammalian Cells

2014 ◽  
Vol 37 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Maximilian Wei-Lin Popp ◽  
Lynne E. Maquat
Keyword(s):  
Mammalian Cells ◽  
Mrna Decay ◽  
Nonsense Mediated Mrna Decay
Sign in / Sign up

Export Citation Format

Share Document